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The map label for this gene is dnaN [H]

Identifier: 121998016

GI number: 121998016

Start: 1332254

End: 1333354

Strand: Reverse

Name: dnaN [H]

Synonym: Hhal_1226

Alternate gene names: 121998016

Gene position: 1333354-1332254 (Counterclockwise)

Preceding gene: 121998017

Following gene: 121998015

Centisome position: 49.78

GC content: 64.76

Gene sequence:

>1101_bases
ATGCGCTTCGAGGTCCAACGCGAACCCCTGCTCGCCGCCCTCCAGTCGATCGTCGGCGTCGTGGAACGCCGCCAGACCCT
GCCGGTTCTTGGCAACATCCGCGTCGAAGCCCGATCGCAACAGCTGCAACTCACCGCCACCGACCTCGAGGTGGAACTCG
TCGCGTACCTTGAGGCCAACACGAAAGACGAAGGTGTGATCACGCTTCCAGCACGGAAATGGCTGGATATATGTCGAAAT
CTTCCGGAAGGTGCGTCGATCAGCATCGAAGCCAATCAGGATCGGGCGACCCTGCGCGCCGCCAGCAGCCGCTTCTCCCT
GGCCACCCTGCCCGCCGATGAATTCCCCGAGGTCGAGTCCATCGGCGACACCGAGGCCGTCGAGATCCCGCGCGGCGAAC
TCCGCCGACTCATCGAGCGGACGCACTTTTCCATGGCGCAGCACGACGTCCGTTACTACCTCAACGGCCTGTTGCTAGAA
CTCACCGGGAGCGGCGCTCGCGCCGTGGCCACCGATGGCCACCGCCTCGCCCTCGCCGAGAGTGACAGCCCGGTCCAAGT
GGCCCAACCCCGCCAAGTCATCGTCCCGCGCAAGGGCGTCCAGGAGTTGCTTCGCCTGCTCGACGATGCCGATGAGCCTG
CCACCCTCGAGTTCGGTACCAACCACGTCCGGGTCACGCTGCAGTCCGTCCGTCTGACGTCCAAGCTGATCGACGGCGTA
TTCCCCGATTACAATCGGGTTATCCCCCAGGATGGCGACAAACACGTCCTGATCGATCGCCAGTACCTGCGCCAGGCCCT
GACCCGGGTGGCCATCCTCTCCAACGAGAAATATCGGGGCATCCGCTTCGCCGTGGAGGGTGACACCCTGCGGATCGGCT
CCCACAACCCGGAACAGGAGGAGGCCGAGGAGGAGCTGGGCATCGAGTACGGTGGCGAGGCCGTGGAACTCGGCTTCAAC
GCCAACTACCTCCTCGATGCCCTCGGTGCCCTGGATACCGAGCGCGTCCAAGTGACCCTGACCGACGCCAGCAGCAGCGG
CCTGATCCGTGCCGAGGGGTCCGACAATGCGCGCTACGTCGTTATGCCGATGCGCCTCTGA

Upstream 100 bases:

>100_bases
ACAGTGTCTTATCAACACTTTCCACAACTTGACGCGGTGCCAATAACAACAACAAGCTTTCTCTTTCCTTAAGCAGTCAT
TACAGCCAACGAGGACGAAC

Downstream 100 bases:

>100_bases
ACCGAATGGAACGCCTGGAGGTCCACGGCTTCCGGAACCTGGCCGATACCCAACTGCGGCCCCATCCCCACCTCAACGTG
GTGACCGGGCCGAATGGCGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRFEVQREPLLAALQSIVGVVERRQTLPVLGNIRVEARSQQLQLTATDLEVELVAYLEANTKDEGVITLPARKWLDICRN
LPEGASISIEANQDRATLRAASSRFSLATLPADEFPEVESIGDTEAVEIPRGELRRLIERTHFSMAQHDVRYYLNGLLLE
LTGSGARAVATDGHRLALAESDSPVQVAQPRQVIVPRKGVQELLRLLDDADEPATLEFGTNHVRVTLQSVRLTSKLIDGV
FPDYNRVIPQDGDKHVLIDRQYLRQALTRVAILSNEKYRGIRFAVEGDTLRIGSHNPEQEEAEEELGIEYGGEAVELGFN
ANYLLDALGALDTERVQVTLTDASSSGLIRAEGSDNARYVVMPMRL

Sequences:

>Translated_366_residues
MRFEVQREPLLAALQSIVGVVERRQTLPVLGNIRVEARSQQLQLTATDLEVELVAYLEANTKDEGVITLPARKWLDICRN
LPEGASISIEANQDRATLRAASSRFSLATLPADEFPEVESIGDTEAVEIPRGELRRLIERTHFSMAQHDVRYYLNGLLLE
LTGSGARAVATDGHRLALAESDSPVQVAQPRQVIVPRKGVQELLRLLDDADEPATLEFGTNHVRVTLQSVRLTSKLIDGV
FPDYNRVIPQDGDKHVLIDRQYLRQALTRVAILSNEKYRGIRFAVEGDTLRIGSHNPEQEEAEEELGIEYGGEAVELGFN
ANYLLDALGALDTERVQVTLTDASSSGLIRAEGSDNARYVVMPMRL
>Mature_366_residues
MRFEVQREPLLAALQSIVGVVERRQTLPVLGNIRVEARSQQLQLTATDLEVELVAYLEANTKDEGVITLPARKWLDICRN
LPEGASISIEANQDRATLRAASSRFSLATLPADEFPEVESIGDTEAVEIPRGELRRLIERTHFSMAQHDVRYYLNGLLLE
LTGSGARAVATDGHRLALAESDSPVQVAQPRQVIVPRKGVQELLRLLDDADEPATLEFGTNHVRVTLQSVRLTSKLIDGV
FPDYNRVIPQDGDKHVLIDRQYLRQALTRVAILSNEKYRGIRFAVEGDTLRIGSHNPEQEEAEEELGIEYGGEAVELGFN
ANYLLDALGALDTERVQVTLTDASSSGLIRAEGSDNARYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=368, Percent_Identity=48.9130434782609, Blast_Score=349, Evalue=2e-97,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40613; Mature: 40613

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFEVQREPLLAALQSIVGVVERRQTLPVLGNIRVEARSQQLQLTATDLEVELVAYLEAN
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCEEEEEEECCEEEEEEEEECC
TKDEGVITLPARKWLDICRNLPEGASISIEANQDRATLRAASSRFSLATLPADEFPEVES
CCCCCEEEECHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCC
IGDTEAVEIPRGELRRLIERTHFSMAQHDVRYYLNGLLLELTGSGARAVATDGHRLALAE
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCEEEEEC
SDSPVQVAQPRQVIVPRKGVQELLRLLDDADEPATLEFGTNHVRVTLQSVRLTSKLIDGV
CCCCEEECCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHCC
FPDYNRVIPQDGDKHVLIDRQYLRQALTRVAILSNEKYRGIRFAVEGDTLRIGSHNPEQE
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCHH
EAEEELGIEYGGEAVELGFNANYLLDALGALDTERVQVTLTDASSSGLIRAEGSDNARYV
HHHHHCCCEECCCEEEECCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCEEE
VMPMRL
EEEECC
>Mature Secondary Structure
MRFEVQREPLLAALQSIVGVVERRQTLPVLGNIRVEARSQQLQLTATDLEVELVAYLEAN
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCEEEEEEECCEEEEEEEEECC
TKDEGVITLPARKWLDICRNLPEGASISIEANQDRATLRAASSRFSLATLPADEFPEVES
CCCCCEEEECHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCC
IGDTEAVEIPRGELRRLIERTHFSMAQHDVRYYLNGLLLELTGSGARAVATDGHRLALAE
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEECCCCEEEEEC
SDSPVQVAQPRQVIVPRKGVQELLRLLDDADEPATLEFGTNHVRVTLQSVRLTSKLIDGV
CCCCEEECCCCEEECCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHCC
FPDYNRVIPQDGDKHVLIDRQYLRQALTRVAILSNEKYRGIRFAVEGDTLRIGSHNPEQE
CCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCHH
EAEEELGIEYGGEAVELGFNANYLLDALGALDTERVQVTLTDASSSGLIRAEGSDNARYV
HHHHHCCCEECCCEEEECCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCEEE
VMPMRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]