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The map label for this gene is phsB [H]

Identifier: 121997955

GI number: 121997955

Start: 1271844

End: 1272623

Strand: Reverse

Name: phsB [H]

Synonym: Hhal_1165

Alternate gene names: 121997955

Gene position: 1272623-1271844 (Counterclockwise)

Preceding gene: 121997956

Following gene: 121997954

Centisome position: 47.51

GC content: 68.33

Gene sequence:

>780_bases
ATGAGCCGCGATTACATCATGCTCGCCGATACAACACGCTGCATCGACTGCAAGGCGTGCGTCGTGGCCTGCCGCGCGGA
GTGGGATGTACCGCTCGGCCATAGCCGGGACTGGGTGGCTAGCGCTGAGACGCGCACCGAGCAGGGGCAGCCGCAGCTGC
ACTTCTTCCCGGGACGATGCCAGCACTGTGATGAGGCGCCGTGCATCGACGCGTGCCCCACGGGTGCCGTGTTCAAACGC
CAGGATGGGCTGGTCCTTCTTGAGCCGGCGCTGTGCTCCGGGTGTGAGCTCTGCGTGCCGGCGTGTCCCTACGGGGCCCG
CTGGCTCGATCCGCGTACCGGGGTGGTGGATTCGTGCACGTTCTGCCAGCCGCTGATCGACGCCGGTCGGCAGCCGGCGT
GCGTCGCCAGTTGTCCGACGGACGCCCTGCTCTTCGGCGATGCGGCGGACCCGGACAGTGAGGTGGCCCGGCGCCTTGAG
GAAGATGGGGACTGGTTCACGCTGAGCACCGACGAGGTCGATTGCAAGCCACGGCACTATTATCGCACCGGTGACCGGGA
GGTGCCCGAGGCGATGCTGCCGGAGCCCCCGGACCCGTATTGGACGCAGCGGGCATCGGAGTCCCTGGTCAATCCGTTGG
CCAAGCTGGGGATCGGTGGCATGGCGGCGCTGTTCGCGGTAGCCGGAGCCAAGCGATTCCTCGACCGCCGGCGCGGGATC
GCGGAGCAGGAGCCGTCGGGCGAGAAGCAGGCGACTCCGCGAGGGGGTGACGATGAGTGA

Upstream 100 bases:

>100_bases
CCACCTTTGCCAGTCCGGATATCGATCCGACCAGTGGTGCCGCGGCCATGCTGCGCGGGCTGGTCCACGTGCGCCGTATT
GAGCCTTCCGAGGGGGTCTC

Downstream 100 bases:

>100_bases
ACAGCTTAAGACGCAGGCGGGGAGTGGTTCGCAGGAGCTGCCGGAGCGGATTCGCATCCGGGACCGGGGCGGCATCGCCC
TGCACTGGTTTAACGCCGGA

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSRDYIMLADTTRCIDCKACVVACRAEWDVPLGHSRDWVASAETRTEQGQPQLHFFPGRCQHCDEAPCIDACPTGAVFKR
QDGLVLLEPALCSGCELCVPACPYGARWLDPRTGVVDSCTFCQPLIDAGRQPACVASCPTDALLFGDAADPDSEVARRLE
EDGDWFTLSTDEVDCKPRHYYRTGDREVPEAMLPEPPDPYWTQRASESLVNPLAKLGIGGMAALFAVAGAKRFLDRRRGI
AEQEPSGEKQATPRGGDDE

Sequences:

>Translated_259_residues
MSRDYIMLADTTRCIDCKACVVACRAEWDVPLGHSRDWVASAETRTEQGQPQLHFFPGRCQHCDEAPCIDACPTGAVFKR
QDGLVLLEPALCSGCELCVPACPYGARWLDPRTGVVDSCTFCQPLIDAGRQPACVASCPTDALLFGDAADPDSEVARRLE
EDGDWFTLSTDEVDCKPRHYYRTGDREVPEAMLPEPPDPYWTQRASESLVNPLAKLGIGGMAALFAVAGAKRFLDRRRGI
AEQEPSGEKQATPRGGDDE
>Mature_258_residues
SRDYIMLADTTRCIDCKACVVACRAEWDVPLGHSRDWVASAETRTEQGQPQLHFFPGRCQHCDEAPCIDACPTGAVFKRQ
DGLVLLEPALCSGCELCVPACPYGARWLDPRTGVVDSCTFCQPLIDAGRQPACVASCPTDALLFGDAADPDSEVARRLEE
DGDWFTLSTDEVDCKPRHYYRTGDREVPEAMLPEPPDPYWTQRASESLVNPLAKLGIGGMAALFAVAGAKRFLDRRRGIA
EQEPSGEKQATPRGGDDE

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=163, Percent_Identity=44.1717791411043, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI226510944, Length=151, Percent_Identity=42.3841059602649, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787872, Length=153, Percent_Identity=34.640522875817, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787122, Length=153, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787749, Length=165, Percent_Identity=33.9393939393939, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790326, Length=249, Percent_Identity=27.710843373494, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1789370, Length=293, Percent_Identity=25.2559726962457, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787740, Length=121, Percent_Identity=32.2314049586777, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787478, Length=87, Percent_Identity=35.632183908046, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789067, Length=166, Percent_Identity=31.9277108433735, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082180, Length=166, Percent_Identity=31.9277108433735, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=29.0502793296089, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=27.2277227722772, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789079, Length=216, Percent_Identity=28.2407407407407, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082179, Length=146, Percent_Identity=32.1917808219178, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=31.1258278145695, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 28340; Mature: 28209

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDYIMLADTTRCIDCKACVVACRAEWDVPLGHSRDWVASAETRTEQGQPQLHFFPGRC
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCCCCCEEECCCCC
QHCDEAPCIDACPTGAVFKRQDGLVLLEPALCSGCELCVPACPYGARWLDPRTGVVDSCT
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHCCCCEECCCCCCCCEECCCCCCCCCCHH
FCQPLIDAGRQPACVASCPTDALLFGDAADPDSEVARRLEEDGDWFTLSTDEVDCKPRHY
HHHHHHHCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCE
YRTGDREVPEAMLPEPPDPYWTQRASESLVNPLAKLGIGGMAALFAVAGAKRFLDRRRGI
ECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
AEQEPSGEKQATPRGGDDE
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRDYIMLADTTRCIDCKACVVACRAEWDVPLGHSRDWVASAETRTEQGQPQLHFFPGRC
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCCCCCEEECCCCC
QHCDEAPCIDACPTGAVFKRQDGLVLLEPALCSGCELCVPACPYGARWLDPRTGVVDSCT
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHCCCCEECCCCCCCCEECCCCCCCCCCHH
FCQPLIDAGRQPACVASCPTDALLFGDAADPDSEVARRLEEDGDWFTLSTDEVDCKPRHY
HHHHHHHCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCE
YRTGDREVPEAMLPEPPDPYWTQRASESLVNPLAKLGIGGMAALFAVAGAKRFLDRRRGI
ECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
AEQEPSGEKQATPRGGDDE
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]