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The map label for this gene is hisH [H]

Identifier: 121997884

GI number: 121997884

Start: 1186459

End: 1187109

Strand: Reverse

Name: hisH [H]

Synonym: Hhal_1093

Alternate gene names: 121997884

Gene position: 1187109-1186459 (Counterclockwise)

Preceding gene: 121997885

Following gene: 121997883

Centisome position: 44.32

GC content: 68.97

Gene sequence:

>651_bases
ATGAGTCGCGTGGCCATTGTCGATTACGGGATGGGCAACCTGCGCTCGGTCTATCGCGCCCTGGCCCACGCCGCCGGCGG
CGAGTCGGGCCTCGAGGTGACCGCCGATCCGGATCAGGTACGGGCCGCCGACCGAGTGGTATTGCCCGGTGTCGGCGCGA
TCGGCGACTGCATGATCGAACTCAACCGCCTGGGGCTGACCGCCGCGCTGATCGAGGCCGCGCGCAGTCGCCCTTTCCTC
GGTATCTGCCTGGGGATGCAGGCGCTGCTGACCTACAGCGAGGAGAGCGGCGGGGTGGATGGCTTGGGCGTCTTGCCGGG
GCGTGCCGTGCGTTTCCCGGACGGCGAGCGTGATGGCCGGGGCCGGCCGCTGAAGGTCCCGCACATGGGTTGGAGCCGGG
TGCACCGGCCCTGCCGCCATCCGCTGTGGGACGGCATCGAGGACGGCACCTGGTTCTACTTCGTGCACAGCTACTACGTG
ATCGCTGACGACCCGGGCATCATCGTCGGTCAGGCCGATCACGGGTGCCGTTTCGCCGCCGCCGTCGCCGATGGCCCCTG
CTTCGCCGTGCAATTCCATCCGGAGAAGAGCCAGGACGCGGGCCTGCGCCTCTATCGCAACTTCCTCGACTGGGACGGGA
GCTGGACGTGA

Upstream 100 bases:

>100_bases
AGTGTCTGTTCAAGGCCTTCGGGCGTGCCCTGCGCCAGGCCGTTAGCACGGATGAGCGTGCCGGTGGTCGGGTTCCTTCC
ACCAAGGGGGCCCTCTAGCC

Downstream 100 bases:

>100_bases
TCGTAATCCCCGCCATCGACCTCAAGAACGGCCACTGCGTGCGCCTGCGCCAGGGCCGGATGGACGACGAGACCATTTTC
GACGCTGACCCCGTCGCGGT

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSRVAIVDYGMGNLRSVYRALAHAAGGESGLEVTADPDQVRAADRVVLPGVGAIGDCMIELNRLGLTAALIEAARSRPFL
GICLGMQALLTYSEESGGVDGLGVLPGRAVRFPDGERDGRGRPLKVPHMGWSRVHRPCRHPLWDGIEDGTWFYFVHSYYV
IADDPGIIVGQADHGCRFAAAVADGPCFAVQFHPEKSQDAGLRLYRNFLDWDGSWT

Sequences:

>Translated_216_residues
MSRVAIVDYGMGNLRSVYRALAHAAGGESGLEVTADPDQVRAADRVVLPGVGAIGDCMIELNRLGLTAALIEAARSRPFL
GICLGMQALLTYSEESGGVDGLGVLPGRAVRFPDGERDGRGRPLKVPHMGWSRVHRPCRHPLWDGIEDGTWFYFVHSYYV
IADDPGIIVGQADHGCRFAAAVADGPCFAVQFHPEKSQDAGLRLYRNFLDWDGSWT
>Mature_215_residues
SRVAIVDYGMGNLRSVYRALAHAAGGESGLEVTADPDQVRAADRVVLPGVGAIGDCMIELNRLGLTAALIEAARSRPFLG
ICLGMQALLTYSEESGGVDGLGVLPGRAVRFPDGERDGRGRPLKVPHMGWSRVHRPCRHPLWDGIEDGTWFYFVHSYYVI
ADDPGIIVGQADHGCRFAAAVADGPCFAVQFHPEKSQDAGLRLYRNFLDWDGSWT

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=44.7115384615385, Blast_Score=136, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=34.2465753424658, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23453; Mature: 23322

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVAIVDYGMGNLRSVYRALAHAAGGESGLEVTADPDQVRAADRVVLPGVGAIGDCMIE
CCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCEEECCCCCCHHHHHHH
LNRLGLTAALIEAARSRPFLGICLGMQALLTYSEESGGVDGLGVLPGRAVRFPDGERDGR
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHEECCCCCCCCCEECCCCCEEECCCCCCCCC
GRPLKVPHMGWSRVHRPCRHPLWDGIEDGTWFYFVHSYYVIADDPGIIVGQADHGCRFAA
CCCEECCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCEEEE
AVADGPCFAVQFHPEKSQDAGLRLYRNFLDWDGSWT
EECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRVAIVDYGMGNLRSVYRALAHAAGGESGLEVTADPDQVRAADRVVLPGVGAIGDCMIE
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHCCEEECCCCCCHHHHHHH
LNRLGLTAALIEAARSRPFLGICLGMQALLTYSEESGGVDGLGVLPGRAVRFPDGERDGR
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHEECCCCCCCCCEECCCCCEEECCCCCCCCC
GRPLKVPHMGWSRVHRPCRHPLWDGIEDGTWFYFVHSYYVIADDPGIIVGQADHGCRFAA
CCCEECCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCEEEE
AVADGPCFAVQFHPEKSQDAGLRLYRNFLDWDGSWT
EECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA