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The map label for this gene is prpC [H]

Identifier: 121997862

GI number: 121997862

Start: 1163487

End: 1164608

Strand: Reverse

Name: prpC [H]

Synonym: Hhal_1071

Alternate gene names: 121997862

Gene position: 1164608-1163487 (Counterclockwise)

Preceding gene: 121997863

Following gene: 121997861

Centisome position: 43.48

GC content: 67.91

Gene sequence:

>1122_bases
ATGACGGGTAAGAAGACGGGTGCCGGGCTGCGCGGCCAATCCGCCGGGGAGACGGCGCTGTGTACGGTGGGGCAGGAGGG
GACCGGCCTGACCTATCGCGGCTACGATGTCCGCGACCTGGCGGCGCAGTGCGAGTTCGAGGAGGTGGCCTACCTGCTGC
TTTACGGCGAGTTGCCGGACCGCGCCGGGCTCGATGCCTACAAGCGCCGCCTGTCGGGCATGCGCGAGCTGCCCCCGGCG
GTCAAGGCGGTGCTCGAACAGATCCCCCGGGACGCCCACCCCATGGACGTGATGCGGACCGGCTGCTCCATGCTCGGTAC
CCTGGAGACCGAGACCGACTTCGCGCAGCAGGCCGACGTCGCCGATCGTCTGCTGGCGGTCTTCCCGTCGATCATCACCT
ACTGGTACCGCTACAGCCACGACGGCGTGCGGATCGACACCGACAGCGATGAGGATTCGATCGGCGGCCACTTCCTCTAC
CTGCTCCGCGGCGAGCGCCCCAACGAGCTGCACAAGGACGTGATGCACGTCTCGCTGATCCTCTACGCGGAGCACGAGTT
CAACGCCTCCACGTTCGCCGCGCGGGTCTGCGCCTCCACGTTGTCCGACATGCACTCGGCGATCACCGGCGCCATCGGGA
CCCTGCGCGGCCCGCTCCACGGCGGCGCCAACGAGGCGGCGATGGAGATGCTCGAGCAGTGGTCGAGCCCCGAGGAAGCG
CGGCGCGAGCTCTCCGGGATGCTCGAGCGCAAGGAGAAGGTGATGGGCTTCGGCCATGCCGTCTATACCGAGGTCGACCC
GCGCAACGCCGTGATCAAGGAGTGGTCCCGGGAACTCGCCGAGGAGGTGGGGGACGACACCCTGTTCCCGGTCTCCGAGG
CCGTGGAGCAGATGATGTGGGACGAGAAGGGCCTGTTCGCCAATGCCGACTTCTACCACGCCTCCGCCTACCGCTTCATG
GGGATCCCCACCAAGCTGTTTACGCCGATCTTCGTGATGTCCCGGCACAGCGGGTGGGCCGCCCACGTCATGGAGCAGCG
GGCGAAGAACCGCATCATCCGCCCGAGCGCCGACTACACCGGCCACACCCTGCGGACGGTGCCGCCGATCGATCGCCGTT
AA

Upstream 100 bases:

>100_bases
AAACGCGCGAGGAACTCTACGAGGTACTCGGTTATCACGACTACGAGCGCAAGCTCGATGAGCTCTTCGGGCGCGACGGA
CGAACAGGAGGAGGACAGCG

Downstream 100 bases:

>100_bases
TGCCGCCGGTTCGTGACCCGCGGTCGCCGTTGCCCGCGACGGCCGGCCCACTGCGACCGCGAAGCGATCAACTTGTAACT
GGTTCTTAAGGAAGCTTGCC

Product: 2-methylcitrate synthase/citrate synthase II

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTGKKTGAGLRGQSAGETALCTVGQEGTGLTYRGYDVRDLAAQCEFEEVAYLLLYGELPDRAGLDAYKRRLSGMRELPPA
VKAVLEQIPRDAHPMDVMRTGCSMLGTLETETDFAQQADVADRLLAVFPSIITYWYRYSHDGVRIDTDSDEDSIGGHFLY
LLRGERPNELHKDVMHVSLILYAEHEFNASTFAARVCASTLSDMHSAITGAIGTLRGPLHGGANEAAMEMLEQWSSPEEA
RRELSGMLERKEKVMGFGHAVYTEVDPRNAVIKEWSRELAEEVGDDTLFPVSEAVEQMMWDEKGLFANADFYHASAYRFM
GIPTKLFTPIFVMSRHSGWAAHVMEQRAKNRIIRPSADYTGHTLRTVPPIDRR

Sequences:

>Translated_373_residues
MTGKKTGAGLRGQSAGETALCTVGQEGTGLTYRGYDVRDLAAQCEFEEVAYLLLYGELPDRAGLDAYKRRLSGMRELPPA
VKAVLEQIPRDAHPMDVMRTGCSMLGTLETETDFAQQADVADRLLAVFPSIITYWYRYSHDGVRIDTDSDEDSIGGHFLY
LLRGERPNELHKDVMHVSLILYAEHEFNASTFAARVCASTLSDMHSAITGAIGTLRGPLHGGANEAAMEMLEQWSSPEEA
RRELSGMLERKEKVMGFGHAVYTEVDPRNAVIKEWSRELAEEVGDDTLFPVSEAVEQMMWDEKGLFANADFYHASAYRFM
GIPTKLFTPIFVMSRHSGWAAHVMEQRAKNRIIRPSADYTGHTLRTVPPIDRR
>Mature_372_residues
TGKKTGAGLRGQSAGETALCTVGQEGTGLTYRGYDVRDLAAQCEFEEVAYLLLYGELPDRAGLDAYKRRLSGMRELPPAV
KAVLEQIPRDAHPMDVMRTGCSMLGTLETETDFAQQADVADRLLAVFPSIITYWYRYSHDGVRIDTDSDEDSIGGHFLYL
LRGERPNELHKDVMHVSLILYAEHEFNASTFAARVCASTLSDMHSAITGAIGTLRGPLHGGANEAAMEMLEQWSSPEEAR
RELSGMLERKEKVMGFGHAVYTEVDPRNAVIKEWSRELAEEVGDDTLFPVSEAVEQMMWDEKGLFANADFYHASAYRFMG
IPTKLFTPIFVMSRHSGWAAHVMEQRAKNRIIRPSADYTGHTLRTVPPIDRR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=336, Percent_Identity=22.6190476190476, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786527, Length=372, Percent_Identity=52.4193548387097, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786939, Length=381, Percent_Identity=33.3333333333333, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=363, Percent_Identity=23.9669421487603, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=359, Percent_Identity=27.2980501392758, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=372, Percent_Identity=25.5376344086022, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640126, Length=353, Percent_Identity=23.5127478753541, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640124, Length=353, Percent_Identity=23.5127478753541, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356863, Length=361, Percent_Identity=22.4376731301939, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 41675; Mature: 41543

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKKTGAGLRGQSAGETALCTVGQEGTGLTYRGYDVRDLAAQCEFEEVAYLLLYGELPD
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHCCHHHHEEHEEECCCCC
RAGLDAYKRRLSGMRELPPAVKAVLEQIPRDAHPMDVMRTGCSMLGTLETETDFAQQADV
CCCHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
ADRLLAVFPSIITYWYRYSHDGVRIDTDSDEDSIGGHFLYLLRGERPNELHKDVMHVSLI
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHE
LYAEHEFNASTFAARVCASTLSDMHSAITGAIGTLRGPLHGGANEAAMEMLEQWSSPEEA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHH
RRELSGMLERKEKVMGFGHAVYTEVDPRNAVIKEWSRELAEEVGDDTLFPVSEAVEQMMW
HHHHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DEKGLFANADFYHASAYRFMGIPTKLFTPIFVMSRHSGWAAHVMEQRAKNRIIRPSADYT
CCCCCEECCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
GHTLRTVPPIDRR
CCEEECCCCCCCC
>Mature Secondary Structure 
TGKKTGAGLRGQSAGETALCTVGQEGTGLTYRGYDVRDLAAQCEFEEVAYLLLYGELPD
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHCCHHHHEEHEEECCCCC
RAGLDAYKRRLSGMRELPPAVKAVLEQIPRDAHPMDVMRTGCSMLGTLETETDFAQQADV
CCCHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
ADRLLAVFPSIITYWYRYSHDGVRIDTDSDEDSIGGHFLYLLRGERPNELHKDVMHVSLI
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHE
LYAEHEFNASTFAARVCASTLSDMHSAITGAIGTLRGPLHGGANEAAMEMLEQWSSPEEA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHH
RRELSGMLERKEKVMGFGHAVYTEVDPRNAVIKEWSRELAEEVGDDTLFPVSEAVEQMMW
HHHHHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DEKGLFANADFYHASAYRFMGIPTKLFTPIFVMSRHSGWAAHVMEQRAKNRIIRPSADYT
CCCCCEECCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
GHTLRTVPPIDRR
CCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]