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The map label for this gene is prpD

Identifier: 121997861

GI number: 121997861

Start: 1161940

End: 1163385

Strand: Reverse

Name: prpD

Synonym: Hhal_1070

Alternate gene names: 121997861

Gene position: 1163385-1161940 (Counterclockwise)

Preceding gene: 121997862

Following gene: 121997858

Centisome position: 43.43

GC content: 69.64

Gene sequence:

>1446_bases
ATGTCCGGAGACATCCGCAGCGCCCAGCGCCCCGACCCCGATGCCGAGCTGGTGGCCATCGCCGACTACGTCAGCGGGAC
CGCCATCGACTCCGCCGAGGCCTACCAGACCGCCCGCCACTGCCTGATGGACAGCCTCGCCTGCGCCATGATGGCGCTCG
ACTACCCGGCCTGTACCAAACTGCTCGGCCCCATCGTCCCGGGGGCCGAGATGCGCGACGGGGTGGTGGTGCCGGGCACC
GCCCATCGGCTCGATCCCGTGCAGGGGGCGTTCAATATCGGCGCGCTGATCCGCTGGCTGGACTTCAACGATACCTGGCT
GGCGGCGGAGTGGGGCCACCCTTCGGATAACCTCGGTGGCATCCTGGCGGCGGCCGATTACGAGAGCCGTCGGCGCCTGG
CGCTGGGTCAGACACCGCTGACCATGCGCGAGGTGCTGACTGCGGCCATCAAGGCCCACGAGATCCAGGGCGTGCTGGCG
CTGGAGAACAGCTTCAACCGTGTCGGCCTCGACCACGTCCTCCTGGTGCGACTGGCGACCACCGCGGTGGCCACGCAGCT
GCTCGGCGGCAGCCGCGAGGACATCATCAACGCCGTCTCCAACGCCTGGATCGACGGCGGCGCCCTGCGGACCTATCGCC
ACGCCCCGAACACCGGCTCGCGCAAGAGCTGGGCCGCCGGGGACGCCTCGGCCCGCGGGGTCCGGCTGGCGCTGATGAGC
CTCACCGGCGAGATGGGCTACCCGTCGGCACTCACCGCCCAGGGGTGGGGCTTCCAGGACGTGCTCTTCCGCGGGGAACC
GATCCGCCGCTCCCAGGCGTACGCCAGTTACGTCATGGAGCACGTGCTGTTCAAGATCTCCTTCCCCGCCGAGTTCCACG
CTCAGACCGCGGTGGAGGCGGCCCTGCAGCTGCACGCCGAGGTGGCGCCTCGACTCGACGAGGTCGACCGGGTGGTCATC
GAGACCCAGGAGGCGGGGGTGCGGATCATCGACAAGACCGGCCCGCTGAACAATCCTGCCGACCGCGACCACTGTCTCCA
GTACATGGTGGCGGTGCCGCTGATCTTCGGCCGCCTGACCGCCGCCGACTACGAGGATGCGGTGGCGGCCGACCCGCGTA
TCGACGCCCTGCGCGAGCGCATGGAGGTCTCCGAGAACGAGGCCTTCTCCCGGGACTACATGGACCCGGAGAAGCGCTCC
ATCGGGAACGCCGTGCAGGTCTTCTTCAAGGATGGCTCCAAGACCGAGCGCATCGCCGTGGAGTACCCCATCGGCCACCG
GCGTCGCCGTGACGAGGGGATCCCGGTGCTGGAGGAGAAGTTCCGCAACGCCCTGGCGGCGCGGTTCGCCCCGCGGCAGG
CTGGCGCCATCGAGGCGGCACTGTCCGACCAGGCGGGTCTGGAACAGATGCCGGTCCACGCCTTTATGGATCTGTGGCGG
GCCTGA

Upstream 100 bases:

>100_bases
GCCGCCGGTTCGTGACCCGCGGTCGCCGTTGCCCGCGACGGCCGGCCCACTGCGACCGCGAAGCGATCAACTTGTAACTG
GTTCTTAAGGAAGCTTGCCC

Downstream 100 bases:

>100_bases
CCCGCCGGTCGCCGCCCCCCCCCCCCCCCGGCGGGCACGACGCCCGCCGGGGGGGGGGTCAGAGATCGAAGAAGCCGCCG
AAATCGTCGAATAGCCCGCC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSGDIRSAQRPDPDAELVAIADYVSGTAIDSAEAYQTARHCLMDSLACAMMALDYPACTKLLGPIVPGAEMRDGVVVPGT
AHRLDPVQGAFNIGALIRWLDFNDTWLAAEWGHPSDNLGGILAAADYESRRRLALGQTPLTMREVLTAAIKAHEIQGVLA
LENSFNRVGLDHVLLVRLATTAVATQLLGGSREDIINAVSNAWIDGGALRTYRHAPNTGSRKSWAAGDASARGVRLALMS
LTGEMGYPSALTAQGWGFQDVLFRGEPIRRSQAYASYVMEHVLFKISFPAEFHAQTAVEAALQLHAEVAPRLDEVDRVVI
ETQEAGVRIIDKTGPLNNPADRDHCLQYMVAVPLIFGRLTAADYEDAVAADPRIDALRERMEVSENEAFSRDYMDPEKRS
IGNAVQVFFKDGSKTERIAVEYPIGHRRRRDEGIPVLEEKFRNALAARFAPRQAGAIEAALSDQAGLEQMPVHAFMDLWR
A

Sequences:

>Translated_481_residues
MSGDIRSAQRPDPDAELVAIADYVSGTAIDSAEAYQTARHCLMDSLACAMMALDYPACTKLLGPIVPGAEMRDGVVVPGT
AHRLDPVQGAFNIGALIRWLDFNDTWLAAEWGHPSDNLGGILAAADYESRRRLALGQTPLTMREVLTAAIKAHEIQGVLA
LENSFNRVGLDHVLLVRLATTAVATQLLGGSREDIINAVSNAWIDGGALRTYRHAPNTGSRKSWAAGDASARGVRLALMS
LTGEMGYPSALTAQGWGFQDVLFRGEPIRRSQAYASYVMEHVLFKISFPAEFHAQTAVEAALQLHAEVAPRLDEVDRVVI
ETQEAGVRIIDKTGPLNNPADRDHCLQYMVAVPLIFGRLTAADYEDAVAADPRIDALRERMEVSENEAFSRDYMDPEKRS
IGNAVQVFFKDGSKTERIAVEYPIGHRRRRDEGIPVLEEKFRNALAARFAPRQAGAIEAALSDQAGLEQMPVHAFMDLWR
A
>Mature_480_residues
SGDIRSAQRPDPDAELVAIADYVSGTAIDSAEAYQTARHCLMDSLACAMMALDYPACTKLLGPIVPGAEMRDGVVVPGTA
HRLDPVQGAFNIGALIRWLDFNDTWLAAEWGHPSDNLGGILAAADYESRRRLALGQTPLTMREVLTAAIKAHEIQGVLAL
ENSFNRVGLDHVLLVRLATTAVATQLLGGSREDIINAVSNAWIDGGALRTYRHAPNTGSRKSWAAGDASARGVRLALMSL
TGEMGYPSALTAQGWGFQDVLFRGEPIRRSQAYASYVMEHVLFKISFPAEFHAQTAVEAALQLHAEVAPRLDEVDRVVIE
TQEAGVRIIDKTGPLNNPADRDHCLQYMVAVPLIFGRLTAADYEDAVAADPRIDALRERMEVSENEAFSRDYMDPEKRSI
GNAVQVFFKDGSKTERIAVEYPIGHRRRRDEGIPVLEEKFRNALAARFAPRQAGAIEAALSDQAGLEQMPVHAFMDLWRA

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=482, Percent_Identity=64.5228215767635, Blast_Score=645, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=489, Percent_Identity=52.5562372188139, Blast_Score=519, Evalue=1e-148,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52585; Mature: 52454

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDIRSAQRPDPDAELVAIADYVSGTAIDSAEAYQTARHCLMDSLACAMMALDYPACTK
CCCCCCCCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LLGPIVPGAEMRDGVVVPGTAHRLDPVQGAFNIGALIRWLDFNDTWLAAEWGHPSDNLGG
HHHCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEEECCCCCCCCCC
ILAAADYESRRRLALGQTPLTMREVLTAAIKAHEIQGVLALENSFNRVGLDHVLLVRLAT
EEEECCHHHCCCEECCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHH
TAVATQLLGGSREDIINAVSNAWIDGGALRTYRHAPNTGSRKSWAAGDASARGVRLALMS
HHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCHHEEEEEH
LTGEMGYPSALTAQGWGFQDVLFRGEPIRRSQAYASYVMEHVLFKISFPAEFHAQTAVEA
HHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHH
ALQLHAEVAPRLDEVDRVVIETQEAGVRIIDKTGPLNNPADRDHCLQYMVAVPLIFGRLT
HHHHHHHHHCCHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AADYEDAVAADPRIDALRERMEVSENEAFSRDYMDPEKRSIGNAVQVFFKDGSKTERIAV
HCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCEEEEEECCCCCCCEEEE
EYPIGHRRRRDEGIPVLEEKFRNALAARFAPRQAGAIEAALSDQAGLEQMPVHAFMDLWR
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHCCHHHHHHHHC
A
C
>Mature Secondary Structure 
SGDIRSAQRPDPDAELVAIADYVSGTAIDSAEAYQTARHCLMDSLACAMMALDYPACTK
CCCCCCCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LLGPIVPGAEMRDGVVVPGTAHRLDPVQGAFNIGALIRWLDFNDTWLAAEWGHPSDNLGG
HHHCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCCCEEEEECCCCCCCCCC
ILAAADYESRRRLALGQTPLTMREVLTAAIKAHEIQGVLALENSFNRVGLDHVLLVRLAT
EEEECCHHHCCCEECCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHH
TAVATQLLGGSREDIINAVSNAWIDGGALRTYRHAPNTGSRKSWAAGDASARGVRLALMS
HHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHEECCCCCCCCCCCCCCCCCCCHHEEEEEH
LTGEMGYPSALTAQGWGFQDVLFRGEPIRRSQAYASYVMEHVLFKISFPAEFHAQTAVEA
HHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHH
ALQLHAEVAPRLDEVDRVVIETQEAGVRIIDKTGPLNNPADRDHCLQYMVAVPLIFGRLT
HHHHHHHHHCCHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AADYEDAVAADPRIDALRERMEVSENEAFSRDYMDPEKRSIGNAVQVFFKDGSKTERIAV
HCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCEEEEEECCCCCCCEEEE
EYPIGHRRRRDEGIPVLEEKFRNALAARFAPRQAGAIEAALSDQAGLEQMPVHAFMDLWR
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHCCHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]