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The map label for this gene is pykA [H]

Identifier: 121997831

GI number: 121997831

Start: 1122884

End: 1124320

Strand: Reverse

Name: pykA [H]

Synonym: Hhal_1040

Alternate gene names: 121997831

Gene position: 1124320-1122884 (Counterclockwise)

Preceding gene: 121997832

Following gene: 121997830

Centisome position: 41.98

GC content: 70.56

Gene sequence:

>1437_bases
ATGATGCCCCGCAGAACCAAGATCCTGGCGACCCTCGGGCCGGCGACGGATCGCCCCGAAGGGCTGGAGGCGGTCCTCGC
CGCCGGGGTGGATGTGGTCCGCATCAACCTCTCCCACGGCGAGGCCAGCGACCACCGCCGGCGGGTCGAGGCCGTCCGGA
CCTGGGGCGAAGCCCACGGCCGGCGGGTGGGCGTGCTGGTGGACCTGCAGGGGCCGAAGATCCGGATTGAGCGCTTTCGC
AATGGCCCGGTCCTCCTGCGCCGCGGGGACCCCTTCATCCTCGATCCTACCGTGGATCCCGATGCCGGGGACCAGACGCA
GGTCGGGGTCGCCTACCGGGCCCTGCCCGACGACCTGCACCCCGGCGACGAGCTGCTGCTCGACGACGGCCGCCTGGTGG
TGCGGGTCGAACGCATCGAGGGGACCGCGGTGCACACCAGCGTGCAGGTCGGCGGTGAACTCAGCGATCGCAAGGGCATC
AACCGCCGCGGTGGCGGGCTTTCGGCGCCGGCGCTCACCGACAAGGACCGGGTCGACATCGTCACCGCCGCCGAACTGGC
CGCCGACTACGTGGCGGTGTCGTTCCCGCGGTGTGCGGACGATATCCACGAGGCCCGCCGGCTGCTGACCGAGGCCGGTG
GGCGGTCCGGGATCGTGGCCAAGATCGAGCGGGCCGAGGCCCTGGACGCCGCCGAGGAGATCATGGATGCCGCCGACGCC
ATCATGGTCGCCCGCGGCGATCTCGGCGTGGAGATCGGCGACGCCGCCCTGCCCGAGGTTCAGAAGCAGCTCATCCAGAC
CGCCCGGGCCCGGAACCGGGTCGCCATCACGGCGACCCAGATGATGGAGTCGATGATCGACAATCCCATCCCCACCCGGG
CCGAGGTCTTCGACGTTGCCAACGCCGTGCGGGATGGCACCGACGCCGTGATGCTCTCGGCAGAGACCGCCACCGGCTCG
TTCCCGGCCGAGACCGTGGCCGCCATGGACCGCGTCTGCCGGGCCGCCGAGCGCAGCCGTACGGTGACGGTCTCCCACCA
CCGGCTGGACGAGCACTTCACGCAGGTCGACGAGACGGTGGCCATGGCGGCGATGTACGCCGCCAACCACTTCGCCATCA
AGGCGCTGATCGCCATCACCGAGTCCGGAGGGACGGCCTCGTGGATGTCGCGGATCAGCTCCGGGCTGCCGATCTACGTC
CTCACCCGCCACCCGGAGACCCGGGGCCGGGTCACCCTCTACCGTGGCGTCTACCCGCTGGAGTTCGATGCCGAGCAGGA
GGACCTCAACGCCCTCAAGAGTCGGCTGCTGGCCCGTCTCTCCAAGCAAGGGCTGGTCAGCCAGGGGGACTACGTCCTGG
TGACCCACGGCGAGGAGCTCGGCGCCGCAGGGGGCACGAACACGTTGCGCATCGTCTGCGTGGACGATTATCTCTAG

Upstream 100 bases:

>100_bases
AGTTCCTCGAGGGGCGCGTTCTGCCCGGCGTTGCGGCCCTGGAGCAGCACGCGGCAGCGCACTCGTGATCGACGCGGTAA
TCAGGCAGGGAGCCGGTGGC

Downstream 100 bases:

>100_bases
AGTGAATGCATAGATAGCGGAGGAGTCGAAACTATGGCGATGATCACCCTGCGACAGCTGCTCGATCACGCAGCCGAGCA
CGGCTACGGCATGCCGGCGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MMPRRTKILATLGPATDRPEGLEAVLAAGVDVVRINLSHGEASDHRRRVEAVRTWGEAHGRRVGVLVDLQGPKIRIERFR
NGPVLLRRGDPFILDPTVDPDAGDQTQVGVAYRALPDDLHPGDELLLDDGRLVVRVERIEGTAVHTSVQVGGELSDRKGI
NRRGGGLSAPALTDKDRVDIVTAAELAADYVAVSFPRCADDIHEARRLLTEAGGRSGIVAKIERAEALDAAEEIMDAADA
IMVARGDLGVEIGDAALPEVQKQLIQTARARNRVAITATQMMESMIDNPIPTRAEVFDVANAVRDGTDAVMLSAETATGS
FPAETVAAMDRVCRAAERSRTVTVSHHRLDEHFTQVDETVAMAAMYAANHFAIKALIAITESGGTASWMSRISSGLPIYV
LTRHPETRGRVTLYRGVYPLEFDAEQEDLNALKSRLLARLSKQGLVSQGDYVLVTHGEELGAAGGTNTLRIVCVDDYL

Sequences:

>Translated_478_residues
MMPRRTKILATLGPATDRPEGLEAVLAAGVDVVRINLSHGEASDHRRRVEAVRTWGEAHGRRVGVLVDLQGPKIRIERFR
NGPVLLRRGDPFILDPTVDPDAGDQTQVGVAYRALPDDLHPGDELLLDDGRLVVRVERIEGTAVHTSVQVGGELSDRKGI
NRRGGGLSAPALTDKDRVDIVTAAELAADYVAVSFPRCADDIHEARRLLTEAGGRSGIVAKIERAEALDAAEEIMDAADA
IMVARGDLGVEIGDAALPEVQKQLIQTARARNRVAITATQMMESMIDNPIPTRAEVFDVANAVRDGTDAVMLSAETATGS
FPAETVAAMDRVCRAAERSRTVTVSHHRLDEHFTQVDETVAMAAMYAANHFAIKALIAITESGGTASWMSRISSGLPIYV
LTRHPETRGRVTLYRGVYPLEFDAEQEDLNALKSRLLARLSKQGLVSQGDYVLVTHGEELGAAGGTNTLRIVCVDDYL
>Mature_478_residues
MMPRRTKILATLGPATDRPEGLEAVLAAGVDVVRINLSHGEASDHRRRVEAVRTWGEAHGRRVGVLVDLQGPKIRIERFR
NGPVLLRRGDPFILDPTVDPDAGDQTQVGVAYRALPDDLHPGDELLLDDGRLVVRVERIEGTAVHTSVQVGGELSDRKGI
NRRGGGLSAPALTDKDRVDIVTAAELAADYVAVSFPRCADDIHEARRLLTEAGGRSGIVAKIERAEALDAAEEIMDAADA
IMVARGDLGVEIGDAALPEVQKQLIQTARARNRVAITATQMMESMIDNPIPTRAEVFDVANAVRDGTDAVMLSAETATGS
FPAETVAAMDRVCRAAERSRTVTVSHHRLDEHFTQVDETVAMAAMYAANHFAIKALIAITESGGTASWMSRISSGLPIYV
LTRHPETRGRVTLYRGVYPLEFDAEQEDLNALKSRLLARLSKQGLVSQGDYVLVTHGEELGAAGGTNTLRIVCVDDYL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=36.1788617886179, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=36.1788617886179, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=35.2822580645161, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=34.2741935483871, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=34.2741935483871, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=33.5640138408304, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=33.5640138408304, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=32.0675105485232, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=32.0675105485232, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=31.0185185185185, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=55.8823529411765, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=36.5957446808511, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=36.701030927835, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=36.1963190184049, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=36.1963190184049, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=36.1963190184049, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=36.1963190184049, Blast_Score=248, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=33.8842975206612, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=32.3770491803279, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=33.8742393509128, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=33.8742393509128, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=34.3137254901961, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.0838445807771, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24646914, Length=277, Percent_Identity=34.2960288808664, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51648; Mature: 51648

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRRTKILATLGPATDRPEGLEAVLAAGVDVVRINLSHGEASDHRRRVEAVRTWGEAHG
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
RRVGVLVDLQGPKIRIERFRNGPVLLRRGDPFILDPTVDPDAGDQTQVGVAYRALPDDLH
CEEEEEEECCCCEEEEEEECCCCEEEECCCCEEECCCCCCCCCCCCEECEEEECCCCCCC
PGDELLLDDGRLVVRVERIEGTAVHTSVQVGGELSDRKGINRRGGGLSAPALTDKDRVDI
CCCCEEEECCEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VTAAELAADYVAVSFPRCADDIHEARRLLTEAGGRSGIVAKIERAEALDAAEEIMDAADA
EEHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHE
IMVARGDLGVEIGDAALPEVQKQLIQTARARNRVAITATQMMESMIDNPIPTRAEVFDVA
EEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHH
NAVRDGTDAVMLSAETATGSFPAETVAAMDRVCRAAERSRTVTVSHHRLDEHFTQVDETV
HHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
AMAAMYAANHFAIKALIAITESGGTASWMSRISSGLPIYVLTRHPETRGRVTLYRGVYPL
HHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECEEEE
EFDAEQEDLNALKSRLLARLSKQGLVSQGDYVLVTHGEELGAAGGTNTLRIVCVDDYL
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MMPRRTKILATLGPATDRPEGLEAVLAAGVDVVRINLSHGEASDHRRRVEAVRTWGEAHG
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
RRVGVLVDLQGPKIRIERFRNGPVLLRRGDPFILDPTVDPDAGDQTQVGVAYRALPDDLH
CEEEEEEECCCCEEEEEEECCCCEEEECCCCEEECCCCCCCCCCCCEECEEEECCCCCCC
PGDELLLDDGRLVVRVERIEGTAVHTSVQVGGELSDRKGINRRGGGLSAPALTDKDRVDI
CCCCEEEECCEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VTAAELAADYVAVSFPRCADDIHEARRLLTEAGGRSGIVAKIERAEALDAAEEIMDAADA
EEHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHE
IMVARGDLGVEIGDAALPEVQKQLIQTARARNRVAITATQMMESMIDNPIPTRAEVFDVA
EEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCHHHHHHHH
NAVRDGTDAVMLSAETATGSFPAETVAAMDRVCRAAERSRTVTVSHHRLDEHFTQVDETV
HHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHH
AMAAMYAANHFAIKALIAITESGGTASWMSRISSGLPIYVLTRHPETRGRVTLYRGVYPL
HHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECEEEE
EFDAEQEDLNALKSRLLARLSKQGLVSQGDYVLVTHGEELGAAGGTNTLRIVCVDDYL
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]