Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 121997832

GI number: 121997832

Start: 1124353

End: 1125537

Strand: Reverse

Name: pgk

Synonym: Hhal_1041

Alternate gene names: 121997832

Gene position: 1125537-1124353 (Counterclockwise)

Preceding gene: 121997833

Following gene: 121997831

Centisome position: 42.02

GC content: 69.54

Gene sequence:

>1185_bases
GTGAAAGCGAAGCGCATGACCGATCTGGACCTGACGGGCAAGCGGGTGCTCATCCGCGAGGATCTGAACGTGCCGATCAA
GGACGGCCAGGTTGCCGACGACACCCGCGTGCGCGCTGCCGCCGAGAGCATCCGCCAGGCCATGCAGGCCGGTGGCCGGG
TGCTGGTGATGTCCCACCTGGGGCGGCCCAAAGAGGGCGAGTACGACGCCGAGGCCTCGATGGCCCCGGTGGCCCGCCGC
CTGGGCGAGATTCTCGGCTGTGAGGTGCCGGTGGTGCGTGACTGGCTGGAGGGCGTGGATGTCCCCGAGGGCGGGGTCGC
GCTGGCGGAGAACGTGCGTTTCCAGCCCGGCGAGACCAAGGACGACGAGGCCCTGTCCCGGCGTATGGCAGCGCTCTGTG
ACGTCTTCGTCATGGACGCCTTCGGCACTGCGCACCGGGCCCAGGCCTCCACCCACGGTGTGGCCCGCTTCGCCCCCGAG
GCCTGTGCAGGCCCGCTGCTCAGTGCCGAGCTGGAGGCCTTGGGCAAGGCTCTGGATAACCCGGCCCGGCCGATGATCGC
CATCGTTGGCGGTTCCAAGGTCTCCGGCAAGGTCCAGGTCCTGGAGGCGCTCACCCACAAAGTCGATCAGCTCATCGTCG
GCGGCGGGATTGCGAACACCTTCATCGCCGCGGCGGGCTACTCTGTGGGCAAGTCCCTCTACGAGGCCGACTTCGTCGAC
ACCGCCAAGCGTCTGATGGAAGAGGCGCGGGCCAAGGGCGGCGAGATCCCGATCCCCGAGGACGTGGTCACGGCCAGGGA
TTTCTCGGCGGACGCCGAGGCCCACGTCCATCCGGTGGACGCCGTGCCCGACGACGAGATGATCCTCGACGTTGGGCCGC
AGACCCGGGCCCGCTACGACGGCATGTTGCGCAACGCCGGTACGGTGGTCTGGAACGGGCCGGTGGGGGTCTTCGAGATG
GCGCCCTTTGCCGGCGGCACCCGGGCGCTGGCCGAGGCCATTGCCGCCAGTGACGGTTTCTCCATTGCTGGTGGCGGGGA
CACGCTGGCCGCGGTGGAGCAGTTCGGCATCACCGACCAGGTCTCGTACATCTCCACCGGCGGTGGCGCCTTCCTGGAGT
TCCTCGAGGGGCGCGTTCTGCCCGGCGTTGCGGCCCTGGAGCAGCACGCGGCAGCGCACTCGTGA

Upstream 100 bases:

>100_bases
CGGCCTGACCGCGGACAACGTCGCCACTGCGGGTCGGCGCGTCCTCGGCTGAGGGCGCCGGCCCGAGCGCAGAACAAGAA
TCGACGAGAGGAGAGTGGCC

Downstream 100 bases:

>100_bases
TCGACGCGGTAATCAGGCAGGGAGCCGGTGGCATGATGCCCCGCAGAACCAAGATCCTGGCGACCCTCGGGCCGGCGACG
GATCGCCCCGAAGGGCTGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKAKRMTDLDLTGKRVLIREDLNVPIKDGQVADDTRVRAAAESIRQAMQAGGRVLVMSHLGRPKEGEYDAEASMAPVARR
LGEILGCEVPVVRDWLEGVDVPEGGVALAENVRFQPGETKDDEALSRRMAALCDVFVMDAFGTAHRAQASTHGVARFAPE
ACAGPLLSAELEALGKALDNPARPMIAIVGGSKVSGKVQVLEALTHKVDQLIVGGGIANTFIAAAGYSVGKSLYEADFVD
TAKRLMEEARAKGGEIPIPEDVVTARDFSADAEAHVHPVDAVPDDEMILDVGPQTRARYDGMLRNAGTVVWNGPVGVFEM
APFAGGTRALAEAIAASDGFSIAGGGDTLAAVEQFGITDQVSYISTGGGAFLEFLEGRVLPGVAALEQHAAAHS

Sequences:

>Translated_394_residues
MKAKRMTDLDLTGKRVLIREDLNVPIKDGQVADDTRVRAAAESIRQAMQAGGRVLVMSHLGRPKEGEYDAEASMAPVARR
LGEILGCEVPVVRDWLEGVDVPEGGVALAENVRFQPGETKDDEALSRRMAALCDVFVMDAFGTAHRAQASTHGVARFAPE
ACAGPLLSAELEALGKALDNPARPMIAIVGGSKVSGKVQVLEALTHKVDQLIVGGGIANTFIAAAGYSVGKSLYEADFVD
TAKRLMEEARAKGGEIPIPEDVVTARDFSADAEAHVHPVDAVPDDEMILDVGPQTRARYDGMLRNAGTVVWNGPVGVFEM
APFAGGTRALAEAIAASDGFSIAGGGDTLAAVEQFGITDQVSYISTGGGAFLEFLEGRVLPGVAALEQHAAAHS
>Mature_394_residues
MKAKRMTDLDLTGKRVLIREDLNVPIKDGQVADDTRVRAAAESIRQAMQAGGRVLVMSHLGRPKEGEYDAEASMAPVARR
LGEILGCEVPVVRDWLEGVDVPEGGVALAENVRFQPGETKDDEALSRRMAALCDVFVMDAFGTAHRAQASTHGVARFAPE
ACAGPLLSAELEALGKALDNPARPMIAIVGGSKVSGKVQVLEALTHKVDQLIVGGGIANTFIAAAGYSVGKSLYEADFVD
TAKRLMEEARAKGGEIPIPEDVVTARDFSADAEAHVHPVDAVPDDEMILDVGPQTRARYDGMLRNAGTVVWNGPVGVFEM
APFAGGTRALAEAIAASDGFSIAGGGDTLAAVEQFGITDQVSYISTGGGAFLEFLEGRVLPGVAALEQHAAAHS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=40.1459854014599, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=38.7019230769231, Blast_Score=267, Evalue=1e-71,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=61.5384615384615, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17508823, Length=405, Percent_Identity=42.2222222222222, Blast_Score=294, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=40.1937046004843, Blast_Score=282, Evalue=7e-77,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=40.6862745098039, Blast_Score=270, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=37.9227053140097, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_HALHL (A1WVV5)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002619.1
- ProteinModelPortal:   A1WVV5
- SMR:   A1WVV5
- STRING:   A1WVV5
- GeneID:   4709793
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1041
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- PhylomeDB:   A1WVV5
- BioCyc:   HHAL349124:HHAL_1041-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41352; Mature: 41352

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKRMTDLDLTGKRVLIREDLNVPIKDGQVADDTRVRAAAESIRQAMQAGGRVLVMSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
GRPKEGEYDAEASMAPVARRLGEILGCEVPVVRDWLEGVDVPEGGVALAENVRFQPGETK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEHHHCCEECCCCCC
DDEALSRRMAALCDVFVMDAFGTAHRAQASTHGVARFAPEACAGPLLSAELEALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHCC
PARPMIAIVGGSKVSGKVQVLEALTHKVDQLIVGGGIANTFIAAAGYSVGKSLYEADFVD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHH
TAKRLMEEARAKGGEIPIPEDVVTARDFSADAEAHVHPVDAVPDDEMILDVGPQTRARYD
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHH
GMLRNAGTVVWNGPVGVFEMAPFAGGTRALAEAIAASDGFSIAGGGDTLAAVEQFGITDQ
HHHHCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCC
VSYISTGGGAFLEFLEGRVLPGVAALEQHAAAHS
HHHEECCHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKAKRMTDLDLTGKRVLIREDLNVPIKDGQVADDTRVRAAAESIRQAMQAGGRVLVMSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
GRPKEGEYDAEASMAPVARRLGEILGCEVPVVRDWLEGVDVPEGGVALAENVRFQPGETK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEHHHCCEECCCCCC
DDEALSRRMAALCDVFVMDAFGTAHRAQASTHGVARFAPEACAGPLLSAELEALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHCC
PARPMIAIVGGSKVSGKVQVLEALTHKVDQLIVGGGIANTFIAAAGYSVGKSLYEADFVD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHH
TAKRLMEEARAKGGEIPIPEDVVTARDFSADAEAHVHPVDAVPDDEMILDVGPQTRARYD
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHH
GMLRNAGTVVWNGPVGVFEMAPFAGGTRALAEAIAASDGFSIAGGGDTLAAVEQFGITDQ
HHHHCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCC
VSYISTGGGAFLEFLEGRVLPGVAALEQHAAAHS
HHHEECCHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA