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The map label for this gene is aceF [H]

Identifier: 121997827

GI number: 121997827

Start: 1116889

End: 1118259

Strand: Reverse

Name: aceF [H]

Synonym: Hhal_1036

Alternate gene names: 121997827

Gene position: 1118259-1116889 (Counterclockwise)

Preceding gene: 121997828

Following gene: 121997826

Centisome position: 41.75

GC content: 71.55

Gene sequence:

>1371_bases
GTGGCGGAACAGGAGCTCAAGGTACCGGATATCGGCGGCTTCGAGGAGGTGGAGGTCATCGAGGTCCTGGTTGCGCCCGG
CGACCGGATCGAGGCCGAGCAGTCGCTGATCACACTGGAGTCCGACAAGGCGAGCATGGAGGTGCCGGCCGAGGTCGGGG
GCGAGATCCGAGCGGTGCATGTGGCCGTGGGGGATACCGTCTCCGAGGGGAGTGTCGTTGCCACCGTTGATCCTGTCGCC
GAGCCGGCGGAACCGGCGACGCAGGCCGAGGCCCCGGCCGCCGCGGGTGGCCCGGCGGAGGAAACGGCCCCGTCGGCCGA
TGGCGGCGCGCCAGCGACCGCGGCCCCCGCCGCCGCGGCGCAACCGGCTGCCTCCGCGGGCAGTGGCGGGGGCGCTGCAG
CCGGAGGTGTCGACGAGTCGCCGGCGATCGACCGCGACGGCCATCGCGCCGCCCACGCCAGCCCCTCGGTACGCCGCTAC
GCCCGCGAGCTCGGGGTCGATCTCTCCCGCGTGCAGGGCAGCGGGCGTAAGGGGCGCATCCGCCGTGAGGACGTGGAGGC
CTACGTCAAGCAGGTGATGCAGGGCCAGGAGGCGCCGCCGGCTGGCGCCGCCGGTGCCCCCGCTGCCGAAGGGGCCGGCA
TCCCGCCGATCCCGGAGCAGGACTTCAGCCGCTTCGGCGAGGTGGAGCGCGTGCCGCTCACCCGTATCCAGCGCCTCTCG
GGGCCGCACCTGCACCGGAGCTGGCTGAATGTCCCGCACGTGACCCAGTTCGACGAGGCCGATATCACCGAGATGGAGGC
GTTCCGCCAATCTCTCAAGAAGGAGGCCGAGGCGCGGGGGGTGAAGCTGACCCCGCTGGCCTTCCTGGTCCGGGCGGCGG
CCGCCGCCCTGGCGGAGTATCCGCGTTTTAACGCCAGCCTTTCGGCGGACGGGCAGGAGCTGATCCTCAAGCACTACTGC
CACATCGGCGTCGCCGTCGACACCCCGGAGGGGCTGGTGGTGCCGGTGCTGCGTGACGCCGACCAGAAAGGCGTCCTGCA
GATCGCCGAGGACCTCGGCACCCTCTCGGCCAAGGCCCGGGACGGCAAGCTCGGTCCGGCGGACATGCAGGGCGGCTGCT
TCTCCATCTCGAGCCTCGGTGGTATCGGCGGTACCGCGTTCACGCCCATTGTCAACGCTCCGGAAGTGGCCATCCTCGGT
GTCTCCCGGTCGCAGACCCGGCCGGTGTGGGATGGGCAGACCTTCCAGCCGCGGCTGATGCTCCCGCTGTCGCTCTCCTA
CGACCACCGGGTCATCGATGGCGCCATGGCTGCCCGCTTCACCAACTACCTGAGCCAGGTCCTCGGCGACCTGCGCCGGC
TGGTGTTGTAG

Upstream 100 bases:

>100_bases
CCCTCGCCGATGAGGGCACCATCGAGGCCGCGAAGGTCAGCGAGGCCATCGGCAAGTACCAGCTGGCGGCGGATGCCCCG
AACCCGTGGGAGGTGTGACC

Downstream 100 bases:

>100_bases
GCAGGCAATCGCTCAGGGGGCGCGGGGGCCGGTGGTCGCCCCCGCGCAAACCACGGCCGGCGCCCTGCGCCGGCGGTCTC
GGTAAGGATTGGAGCACGGC

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAEQELKVPDIGGFEEVEVIEVLVAPGDRIEAEQSLITLESDKASMEVPAEVGGEIRAVHVAVGDTVSEGSVVATVDPVA
EPAEPATQAEAPAAAGGPAEETAPSADGGAPATAAPAAAAQPAASAGSGGGAAAGGVDESPAIDRDGHRAAHASPSVRRY
ARELGVDLSRVQGSGRKGRIRREDVEAYVKQVMQGQEAPPAGAAGAPAAEGAGIPPIPEQDFSRFGEVERVPLTRIQRLS
GPHLHRSWLNVPHVTQFDEADITEMEAFRQSLKKEAEARGVKLTPLAFLVRAAAAALAEYPRFNASLSADGQELILKHYC
HIGVAVDTPEGLVVPVLRDADQKGVLQIAEDLGTLSAKARDGKLGPADMQGGCFSISSLGGIGGTAFTPIVNAPEVAILG
VSRSQTRPVWDGQTFQPRLMLPLSLSYDHRVIDGAMAARFTNYLSQVLGDLRRLVL

Sequences:

>Translated_456_residues
MAEQELKVPDIGGFEEVEVIEVLVAPGDRIEAEQSLITLESDKASMEVPAEVGGEIRAVHVAVGDTVSEGSVVATVDPVA
EPAEPATQAEAPAAAGGPAEETAPSADGGAPATAAPAAAAQPAASAGSGGGAAAGGVDESPAIDRDGHRAAHASPSVRRY
ARELGVDLSRVQGSGRKGRIRREDVEAYVKQVMQGQEAPPAGAAGAPAAEGAGIPPIPEQDFSRFGEVERVPLTRIQRLS
GPHLHRSWLNVPHVTQFDEADITEMEAFRQSLKKEAEARGVKLTPLAFLVRAAAAALAEYPRFNASLSADGQELILKHYC
HIGVAVDTPEGLVVPVLRDADQKGVLQIAEDLGTLSAKARDGKLGPADMQGGCFSISSLGGIGGTAFTPIVNAPEVAILG
VSRSQTRPVWDGQTFQPRLMLPLSLSYDHRVIDGAMAARFTNYLSQVLGDLRRLVL
>Mature_455_residues
AEQELKVPDIGGFEEVEVIEVLVAPGDRIEAEQSLITLESDKASMEVPAEVGGEIRAVHVAVGDTVSEGSVVATVDPVAE
PAEPATQAEAPAAAGGPAEETAPSADGGAPATAAPAAAAQPAASAGSGGGAAAGGVDESPAIDRDGHRAAHASPSVRRYA
RELGVDLSRVQGSGRKGRIRREDVEAYVKQVMQGQEAPPAGAAGAPAAEGAGIPPIPEQDFSRFGEVERVPLTRIQRLSG
PHLHRSWLNVPHVTQFDEADITEMEAFRQSLKKEAEARGVKLTPLAFLVRAAAAALAEYPRFNASLSADGQELILKHYCH
IGVAVDTPEGLVVPVLRDADQKGVLQIAEDLGTLSAKARDGKLGPADMQGGCFSISSLGGIGGTAFTPIVNAPEVAILGV
SRSQTRPVWDGQTFQPRLMLPLSLSYDHRVIDGAMAARFTNYLSQVLGDLRRLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=458, Percent_Identity=29.9126637554585, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI31711992, Length=303, Percent_Identity=32.3432343234323, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=325, Percent_Identity=31.0769230769231, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI203098753, Length=466, Percent_Identity=27.4678111587983, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=36.0869565217391, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=37.7245508982036, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786305, Length=311, Percent_Identity=59.1639871382637, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=30.787037037037, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=29.7297297297297, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=229, Percent_Identity=39.3013100436681, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=29.2517006802721, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=33.6538461538462, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=433, Percent_Identity=29.5612009237875, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=442, Percent_Identity=27.1493212669683, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859875, Length=453, Percent_Identity=31.5673289183223, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.618025751073, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24645909, Length=213, Percent_Identity=35.2112676056338, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47453; Mature: 47322

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQELKVPDIGGFEEVEVIEVLVAPGDRIEAEQSLITLESDKASMEVPAEVGGEIRAVH
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHEEEEECCCCCEECCHHCCCCEEEEE
VAVGDTVSEGSVVATVDPVAEPAEPATQAEAPAAAGGPAEETAPSADGGAPATAAPAAAA
EEECCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHC
QPAASAGSGGGAAAGGVDESPAIDRDGHRAAHASPSVRRYARELGVDLSRVQGSGRKGRI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCC
RREDVEAYVKQVMQGQEAPPAGAAGAPAAEGAGIPPIPEQDFSRFGEVERVPLTRIQRLS
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHCC
GPHLHRSWLNVPHVTQFDEADITEMEAFRQSLKKEAEARGVKLTPLAFLVRAAAAALAEY
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHC
PRFNASLSADGQELILKHYCHIGVAVDTPEGLVVPVLRDADQKGVLQIAEDLGTLSAKAR
CCCCCCCCCCHHHHHHHHHHHEEEEEECCCCEEEEHCCCCCHHHHHHHHHHHHHHHCCCC
DGKLGPADMQGGCFSISSLGGIGGTAFTPIVNAPEVAILGVSRSQTRPVWDGQTFQPRLM
CCCCCCCCCCCCEEEHHHCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
LPLSLSYDHRVIDGAMAARFTNYLSQVLGDLRRLVL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AEQELKVPDIGGFEEVEVIEVLVAPGDRIEAEQSLITLESDKASMEVPAEVGGEIRAVH
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHEEEEECCCCCEECCHHCCCCEEEEE
VAVGDTVSEGSVVATVDPVAEPAEPATQAEAPAAAGGPAEETAPSADGGAPATAAPAAAA
EEECCCCCCCCEEEEECCCCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHC
QPAASAGSGGGAAAGGVDESPAIDRDGHRAAHASPSVRRYARELGVDLSRVQGSGRKGRI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCC
RREDVEAYVKQVMQGQEAPPAGAAGAPAAEGAGIPPIPEQDFSRFGEVERVPLTRIQRLS
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHCC
GPHLHRSWLNVPHVTQFDEADITEMEAFRQSLKKEAEARGVKLTPLAFLVRAAAAALAEY
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHC
PRFNASLSADGQELILKHYCHIGVAVDTPEGLVVPVLRDADQKGVLQIAEDLGTLSAKAR
CCCCCCCCCCHHHHHHHHHHHEEEEEECCCCEEEEHCCCCCHHHHHHHHHHHHHHHCCCC
DGKLGPADMQGGCFSISSLGGIGGTAFTPIVNAPEVAILGVSRSQTRPVWDGQTFQPRLM
CCCCCCCCCCCCEEEHHHCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
LPLSLSYDHRVIDGAMAARFTNYLSQVLGDLRRLVL
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]