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The map label for this gene is lpdA [H]

Identifier: 121997826

GI number: 121997826

Start: 1115007

End: 1116788

Strand: Reverse

Name: lpdA [H]

Synonym: Hhal_1035

Alternate gene names: 121997826

Gene position: 1116788-1115007 (Counterclockwise)

Preceding gene: 121997827

Following gene: 121997825

Centisome position: 41.7

GC content: 69.81

Gene sequence:

>1782_bases
ATGGCTGAGCAACAGGTCAAGGTACCGGATATCGGCGGCTTCGAGGCCGTAGAGGTTATCGAGGTGCTGGTCGGCCCCGG
GGATCGGATCGAGTCCGAACAGTCGCTGATCACGCTGGAGTCCGACAAGGCGAGCATGGAGGTGCCGGCCTCCGCCGCCG
GCGTGGTCCGCGAGGTCCATGTGGCGGTGGGCGACACCGTCTCCGAAGGGGCGCCCATCCTGACCCTCGAGGCGGAGGCG
GAGGCGGACGCGGCAGGTGGCAACGAGGAGCGCGCGGCGCCCCCGGGCGAGTCGGCCGAGGCGCCGTCGCCGGGTCCGGT
CGAGCCGACCGCGCCAGCCCCGGCGGCGGCAGCTGCTGGCGCGCCGCAGGACCCGGACTGCGACGTGGTGGTCCTGGGGG
CGGGGCCGGGCGGCTACAGTGCCGCCTTTCGGGCCGCCGATCTGGGCCTGAAGGTGGTGCTGGTGGATCGCTACCCGGTC
CTCGGCGGCGTCTGCCTCAACGTCGGGTGTATCCCCTCCAAGGCGCTGCTCCACGCCGCCAAGGTGGTGGACGAGGCCGA
TCAATTCGCTGCCCACGGGATCGCCTTCGGCAAGCCGAAGATCGATCTGGAGCAGCTCAACGACTGGAAGCGCGGCATCA
TCAAGCAGCTGACCCAGGGGCTGGCGGGGATCGCCAAGCAGCGCAAGGTCGAGGTGGTCACCGGCGAAGCGGCCTTTGCC
GACGCCCACCACCTGCACGTGTCGGGGGACGACGGCGCACGCACGATCAGCTTCCGGCACTGCATCGTCGCGGCCGGCTC
GCGTCCGGCGATCCCGCCGTCGCTGGCGGTGGACGATCCGCGGGTGATGGACTCCACCGGTGCGCTCAAGCTTGAGGAGA
TCCCGGACCGGCTGTTGGTGGTCGGTGGCGGCATCATCGGTCTGGAGATGGCCTCGGTCTACTCCGGGCTGGGTAGCAAG
GTCACCGTTGTCGAACTCTCCGACCGGCTCATGCCGGGAGCCGATCCGGACCTGGTCAAGGTGCTGCGCAAGCGCCTGGC
CAAACGCTGTGAGGCGATCCACCTGAACACCGAGGTGACCGGGGTCAAGGCCAACAAGAAGTCGCTGACCGTCACCATGA
ACGGTCAGGACGCGCCGGAGAGCGACCGCTTCGATCGCATCCTGGTGGCCGTCGGCCGCCGCCCGAACAGTGACCGGATC
GGGGTTGAGGCCGCCGGCCTGGCGCCGGGGGAGGACGGGTGCCTGAGCGTGGACGAGCACATGCGTACCGCGGTGCCGCA
TATTCACGCCGTGGGCGATATCGTCGGCCAGCCCATGCTGGCGCACAAGGCGGTGCACGAGGGTAAGGTGGCCGCCGAGG
TGATCGCCGGCGAGAAGAGTGCCTGGGACGCCCGGGCCATCCCGTCGGTGGCCTACACCGATCCGGAGGTCGCCTGGGTG
GGGCTGACCGAGGAGCAGGCGAAGGCCGAGGGGGTGGCCTACGAGAAGGGGGCCTTCCCGTGGGCGGCCAATGGCCGTGC
GCTGTCGCTGGATGCCAGTGACGGTGTTTCCAAGATCCTCTTCGATGCGGAGACCGGGCGGGTGCTCGGCGGCGGCATTG
TCGGCCCCGGCGCCGGCGATCTGATCGGCGAGATCGGTCTGGCCCTGGAGATGGGGGCGGACGCCCACGACATCGGTCTG
ACGGTTCATCCCCACCCGACGCTCTCCGAGACGGTGGCTATGGCGGCGGAGATGGCCAGCGGCAGCATCACCGACCTGCT
ACCGCCGAAGAAGCGCAAGTAG

Upstream 100 bases:

>100_bases
GCAGGCAATCGCTCAGGGGGCGCGGGGGCCGGTGGTCGCCCCCGCGCAAACCACGGCCGGCGCCCTGCGCCGGCGGTCTC
GGTAAGGATTGGAGCACGGC

Downstream 100 bases:

>100_bases
TCGCCCGTCGTCGCCCCGATTGCGGGGGGGCCGGGGCTGGTGCGGCGTGGTGGTGAGCGTCTCCGGTCCGGGGGTGCCCC
GCTGGTGCGGCGTCGAACGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAEQQVKVPDIGGFEAVEVIEVLVGPGDRIESEQSLITLESDKASMEVPASAAGVVREVHVAVGDTVSEGAPILTLEAEA
EADAAGGNEERAAPPGESAEAPSPGPVEPTAPAPAAAAAGAPQDPDCDVVVLGAGPGGYSAAFRAADLGLKVVLVDRYPV
LGGVCLNVGCIPSKALLHAAKVVDEADQFAAHGIAFGKPKIDLEQLNDWKRGIIKQLTQGLAGIAKQRKVEVVTGEAAFA
DAHHLHVSGDDGARTISFRHCIVAAGSRPAIPPSLAVDDPRVMDSTGALKLEEIPDRLLVVGGGIIGLEMASVYSGLGSK
VTVVELSDRLMPGADPDLVKVLRKRLAKRCEAIHLNTEVTGVKANKKSLTVTMNGQDAPESDRFDRILVAVGRRPNSDRI
GVEAAGLAPGEDGCLSVDEHMRTAVPHIHAVGDIVGQPMLAHKAVHEGKVAAEVIAGEKSAWDARAIPSVAYTDPEVAWV
GLTEEQAKAEGVAYEKGAFPWAANGRALSLDASDGVSKILFDAETGRVLGGGIVGPGAGDLIGEIGLALEMGADAHDIGL
TVHPHPTLSETVAMAAEMASGSITDLLPPKKRK

Sequences:

>Translated_593_residues
MAEQQVKVPDIGGFEAVEVIEVLVGPGDRIESEQSLITLESDKASMEVPASAAGVVREVHVAVGDTVSEGAPILTLEAEA
EADAAGGNEERAAPPGESAEAPSPGPVEPTAPAPAAAAAGAPQDPDCDVVVLGAGPGGYSAAFRAADLGLKVVLVDRYPV
LGGVCLNVGCIPSKALLHAAKVVDEADQFAAHGIAFGKPKIDLEQLNDWKRGIIKQLTQGLAGIAKQRKVEVVTGEAAFA
DAHHLHVSGDDGARTISFRHCIVAAGSRPAIPPSLAVDDPRVMDSTGALKLEEIPDRLLVVGGGIIGLEMASVYSGLGSK
VTVVELSDRLMPGADPDLVKVLRKRLAKRCEAIHLNTEVTGVKANKKSLTVTMNGQDAPESDRFDRILVAVGRRPNSDRI
GVEAAGLAPGEDGCLSVDEHMRTAVPHIHAVGDIVGQPMLAHKAVHEGKVAAEVIAGEKSAWDARAIPSVAYTDPEVAWV
GLTEEQAKAEGVAYEKGAFPWAANGRALSLDASDGVSKILFDAETGRVLGGGIVGPGAGDLIGEIGLALEMGADAHDIGL
TVHPHPTLSETVAMAAEMASGSITDLLPPKKRK
>Mature_592_residues
AEQQVKVPDIGGFEAVEVIEVLVGPGDRIESEQSLITLESDKASMEVPASAAGVVREVHVAVGDTVSEGAPILTLEAEAE
ADAAGGNEERAAPPGESAEAPSPGPVEPTAPAPAAAAAGAPQDPDCDVVVLGAGPGGYSAAFRAADLGLKVVLVDRYPVL
GGVCLNVGCIPSKALLHAAKVVDEADQFAAHGIAFGKPKIDLEQLNDWKRGIIKQLTQGLAGIAKQRKVEVVTGEAAFAD
AHHLHVSGDDGARTISFRHCIVAAGSRPAIPPSLAVDDPRVMDSTGALKLEEIPDRLLVVGGGIIGLEMASVYSGLGSKV
TVVELSDRLMPGADPDLVKVLRKRLAKRCEAIHLNTEVTGVKANKKSLTVTMNGQDAPESDRFDRILVAVGRRPNSDRIG
VEAAGLAPGEDGCLSVDEHMRTAVPHIHAVGDIVGQPMLAHKAVHEGKVAAEVIAGEKSAWDARAIPSVAYTDPEVAWVG
LTEEQAKAEGVAYEKGAFPWAANGRALSLDASDGVSKILFDAETGRVLGGGIVGPGAGDLIGEIGLALEMGADAHDIGLT
VHPHPTLSETVAMAAEMASGSITDLLPPKKRK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=41.1513859275053, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=28.448275862069, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=29.9568965517241, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=488, Percent_Identity=27.8688524590164, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI291045268, Length=478, Percent_Identity=25.7322175732218, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=58.3690987124464, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=32.1120689655172, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.9120879120879, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=30.6636155606407, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1786305, Length=76, Percent_Identity=60.5263157894737, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=450, Percent_Identity=42.4444444444444, Blast_Score=341, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=29.5744680851064, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=28.5067873303167, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=28.5067873303167, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=25.979381443299, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=42.9824561403509, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=30.2127659574468, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=28.3549783549784, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=451, Percent_Identity=42.350332594235, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=29.5315682281059, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24640551, Length=511, Percent_Identity=29.9412915851272, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=30.4255319148936, Blast_Score=139, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24640553, Length=473, Percent_Identity=30.4439746300211, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61123; Mature: 60992

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQQVKVPDIGGFEAVEVIEVLVGPGDRIESEQSLITLESDKASMEVPASAAGVVREVH
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHH
VAVGDTVSEGAPILTLEAEAEADAAGGNEERAAPPGESAEAPSPGPVEPTAPAPAAAAAG
HHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
APQDPDCDVVVLGAGPGGYSAAFRAADLGLKVVLVDRYPVLGGVCLNVGCIPSKALLHAA
CCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHH
KVVDEADQFAAHGIAFGKPKIDLEQLNDWKRGIIKQLTQGLAGIAKQRKVEVVTGEAAFA
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEC
DAHHLHVSGDDGARTISFRHCIVAAGSRPAIPPSLAVDDPRVMDSTGALKLEEIPDRLLV
CCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEHHCCCCEEEE
VGGGIIGLEMASVYSGLGSKVTVVELSDRLMPGADPDLVKVLRKRLAKRCEAIHLNTEVT
EECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEE
GVKANKKSLTVTMNGQDAPESDRFDRILVAVGRRPNSDRIGVEAAGLAPGEDGCLSVDEH
EEEECCCEEEEEECCCCCCCCHHHCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHHH
MRTAVPHIHAVGDIVGQPMLAHKAVHEGKVAAEVIAGEKSAWDARAIPSVAYTDPEVAWV
HHHHCCHHHHHHHHHCCCHHHHHHHHCCCCEEHHHCCCCCCCCHHCCCCEEECCCCEEEE
GLTEEQAKAEGVAYEKGAFPWAANGRALSLDASDGVSKILFDAETGRVLGGGIVGPGAGD
ECCHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEECCEECCCCHH
LIGEIGLALEMGADAHDIGLTVHPHPTLSETVAMAAEMASGSITDLLPPKKRK
HHHHHCEEEECCCCCHHCEEEECCCCCHHHHHHHHHHHCCCCCHHCCCCCCCC
>Mature Secondary Structure 
AEQQVKVPDIGGFEAVEVIEVLVGPGDRIESEQSLITLESDKASMEVPASAAGVVREVH
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHH
VAVGDTVSEGAPILTLEAEAEADAAGGNEERAAPPGESAEAPSPGPVEPTAPAPAAAAAG
HHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
APQDPDCDVVVLGAGPGGYSAAFRAADLGLKVVLVDRYPVLGGVCLNVGCIPSKALLHAA
CCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHH
KVVDEADQFAAHGIAFGKPKIDLEQLNDWKRGIIKQLTQGLAGIAKQRKVEVVTGEAAFA
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEC
DAHHLHVSGDDGARTISFRHCIVAAGSRPAIPPSLAVDDPRVMDSTGALKLEEIPDRLLV
CCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEHHCCCCEEEE
VGGGIIGLEMASVYSGLGSKVTVVELSDRLMPGADPDLVKVLRKRLAKRCEAIHLNTEVT
EECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEE
GVKANKKSLTVTMNGQDAPESDRFDRILVAVGRRPNSDRIGVEAAGLAPGEDGCLSVDEH
EEEECCCEEEEEECCCCCCCCHHHCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCHHHH
MRTAVPHIHAVGDIVGQPMLAHKAVHEGKVAAEVIAGEKSAWDARAIPSVAYTDPEVAWV
HHHHCCHHHHHHHHHCCCHHHHHHHHCCCCEEHHHCCCCCCCCHHCCCCEEECCCCEEEE
GLTEEQAKAEGVAYEKGAFPWAANGRALSLDASDGVSKILFDAETGRVLGGGIVGPGAGD
ECCHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEECCEECCCCHH
LIGEIGLALEMGADAHDIGLTVHPHPTLSETVAMAAEMASGSITDLLPPKKRK
HHHHHCEEEECCCCCHHCEEEECCCCCHHHHHHHHHHHCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]