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The map label for this gene is lolB

Identifier: 121997782

GI number: 121997782

Start: 1063114

End: 1063725

Strand: Reverse

Name: lolB

Synonym: Hhal_0991

Alternate gene names: 121997782

Gene position: 1063725-1063114 (Counterclockwise)

Preceding gene: 121997783

Following gene: 121997781

Centisome position: 39.71

GC content: 72.55

Gene sequence:

>612_bases
ATGACCGGTAGATGGAGCCCGCGGCTGCTCGCCGGGCTGCTGGCGGCGCTGGTGCTGTCGGGGTGCGCCCTGTTGGTCCC
CGAGGACGAGCGGGAGGCGCAGTACGAGGCCTTTCTGGAGGAGCGTGCCGAGCTGCGAGACTGGTCGGTGGCTGGCCGGG
CAGCGCTGCGCGCCGAGGGTGAGGCGGTGAGCCTCTCCTTACGCTGGGAGCAGCGAGGCGAGGTGTACACCATCAACTTA
TCCGGCCCGTTCGGGGCCGGGGCGGTGCGCATCGAGGGGCAGCCGGGGCGGGTGACCCTGCGGGACGGCGCCGGGCAGAG
CGCCACGGCGCAGAGCCCGGAGGAGCTGCTGGCGGCGCAGACCGGCCACCAGCTGCCCGTGACCGCGCTGCGTGACTGGA
TCGTCGGGCGCCCGGCGGACGGTCTGGAGGTGGACGAGCTGAGCCTGGACCGTGTCGGGCGCCCGGACCGTCTCGAACAG
GCCGGCTGGCGGGTCGATTTCCAGGGGTGGACGGACGTCGATGGGGTCGATCTGCCGAGCCGGGTCGATCTGACCCGCGG
TTCGACGCAGATGCGGGTCGCCCTCTCAGGCTGGAGTCGCAGCGATGACTGA

Upstream 100 bases:

>100_bases
GAGCGCAACGAGGCACGCGAGGTCTGGTGTCGGGCGCTGGATGCCGACGGTGGCCAGCGCGCCCTGAAGCGGACGCTGGA
ACGCTACGAGGTGGATGTCG

Downstream 100 bases:

>100_bases
ATGGAGCGTCTGGCCGGCCCCGGCCAAGATCAACCTCTTTCTCCACGTCCTCGGCCGGCGTGCCGACGGCTACCACGAGC
TGCAGACCGCCTTCCAGCTG

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTGRWSPRLLAGLLAALVLSGCALLVPEDEREAQYEAFLEERAELRDWSVAGRAALRAEGEAVSLSLRWEQRGEVYTINL
SGPFGAGAVRIEGQPGRVTLRDGAGQSATAQSPEELLAAQTGHQLPVTALRDWIVGRPADGLEVDELSLDRVGRPDRLEQ
AGWRVDFQGWTDVDGVDLPSRVDLTRGSTQMRVALSGWSRSDD

Sequences:

>Translated_203_residues
MTGRWSPRLLAGLLAALVLSGCALLVPEDEREAQYEAFLEERAELRDWSVAGRAALRAEGEAVSLSLRWEQRGEVYTINL
SGPFGAGAVRIEGQPGRVTLRDGAGQSATAQSPEELLAAQTGHQLPVTALRDWIVGRPADGLEVDELSLDRVGRPDRLEQ
AGWRVDFQGWTDVDGVDLPSRVDLTRGSTQMRVALSGWSRSDD
>Mature_202_residues
TGRWSPRLLAGLLAALVLSGCALLVPEDEREAQYEAFLEERAELRDWSVAGRAALRAEGEAVSLSLRWEQRGEVYTINLS
GPFGAGAVRIEGQPGRVTLRDGAGQSATAQSPEELLAAQTGHQLPVTALRDWIVGRPADGLEVDELSLDRVGRPDRLEQA
GWRVDFQGWTDVDGVDLPSRVDLTRGSTQMRVALSGWSRSDD

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=204, Percent_Identity=26.9607843137255, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_HALHL (A1WVQ5)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002569.1
- ProteinModelPortal:   A1WVQ5
- SMR:   A1WVQ5
- STRING:   A1WVQ5
- GeneID:   4709563
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0991
- eggNOG:   COG3017
- HOGENOM:   HBG645031
- OMA:   RIEASNG
- PhylomeDB:   A1WVQ5
- ProtClustDB:   CLSK727064
- BioCyc:   HHAL349124:HHAL_0991-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 22106; Mature: 21975

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRWSPRLLAGLLAALVLSGCALLVPEDEREAQYEAFLEERAELRDWSVAGRAALRAEG
CCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCC
EAVSLSLRWEQRGEVYTINLSGPFGAGAVRIEGQPGRVTLRDGAGQSATAQSPEELLAAQ
CEEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHH
TGHQLPVTALRDWIVGRPADGLEVDELSLDRVGRPDRLEQAGWRVDFQGWTDVDGVDLPS
CCCCCCHHHHHHHHCCCCCCCCEECCCCHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCC
RVDLTRGSTQMRVALSGWSRSDD
CEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
TGRWSPRLLAGLLAALVLSGCALLVPEDEREAQYEAFLEERAELRDWSVAGRAALRAEG
CCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCC
EAVSLSLRWEQRGEVYTINLSGPFGAGAVRIEGQPGRVTLRDGAGQSATAQSPEELLAAQ
CEEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHH
TGHQLPVTALRDWIVGRPADGLEVDELSLDRVGRPDRLEQAGWRVDFQGWTDVDGVDLPS
CCCCCCHHHHHHHHCCCCCCCCEECCCCHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCC
RVDLTRGSTQMRVALSGWSRSDD
CEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA