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The map label for this gene is rph

Identifier: 121997764

GI number: 121997764

Start: 1045137

End: 1045853

Strand: Reverse

Name: rph

Synonym: Hhal_0973

Alternate gene names: 121997764

Gene position: 1045853-1045137 (Counterclockwise)

Preceding gene: 121997768

Following gene: 121997763

Centisome position: 39.05

GC content: 71.27

Gene sequence:

>717_bases
ATGCGCCCAAGCGGTCGACGGGCCGACGAGCTACGCCCGATCTCCATCCAGCGCCAGTTCACCAAACACGCCGAGGGCTC
GGTGCTCATCGGCTTTGGCGATACGCGCGTGCTGTGCACGGCCAGCGTCGAGGCGCGCGTGCCCCCCTGGCTGCGCGGCC
AGGGCCGGGGCTGGGTGACCGCCGAGTACGGCATGTTGCCACGCTCGACCCACACCCGCAGCGACCGCGAGGCGGCCCGC
GGCAAGCAGCAAGGGCGGACTGTCGAGATCCAGCGGCTGATCGGCCGCTCCCTGCGCGCCGCCGTCGATCTGCAGGCCCT
GGGCGAGCGTAGCGTGGTGGTCGACTGCGACGTGCTGCAGGCCGATGGCGGGACCCGGACGGCGGCGATCACCGGTGCCT
TCGTGGCCCTGACCGACGCCCTGGAAGGGCTGGTGCGCCAGCGCGCGCTGCCGGCGTCGCCCCTCCACGGTCAGGTGGCG
TCGGTCTCCGTGGGGGTCTACCGCGGTGAGCCGGTGCTCGACCTGGACTATGCCGAGGACGGCGAGGCGGAGACCGACAT
GAACGTGGTGATGAACGACGCCGGCGCCTTCATCGAGCTGCAGGGCACCGCCGAGGGGCACGCCTTCCGGCGCGATGAAC
TCGATCGGATGCTGGCCCTGGCCGAGAAGGGCGTGGGTGAGCTGTTGCAGCATCAGCAGGAGGCATTGGCGACATGA

Upstream 100 bases:

>100_bases
CACAGAAACCCCAACCCGTATAATCCATTACCTGGGCTGTCACGTCCATACTCACCACCCCGGGGGCGGCGAGCACGCCG
CCCCGAATGCAGGAGGCACT

Downstream 100 bases:

>100_bases
AGATCGTGCTGGCCACCGGGAATGCCGGCAAGCTTGCCGAGATGACCCGCATGCTCCAGGGCTACGACGCCGAGGTGGTC
AGGCAGGGGCACCTGGGCAT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSGRRADELRPISIQRQFTKHAEGSVLIGFGDTRVLCTASVEARVPPWLRGQGRGWVTAEYGMLPRSTHTRSDREAAR
GKQQGRTVEIQRLIGRSLRAAVDLQALGERSVVVDCDVLQADGGTRTAAITGAFVALTDALEGLVRQRALPASPLHGQVA
SVSVGVYRGEPVLDLDYAEDGEAETDMNVVMNDAGAFIELQGTAEGHAFRRDELDRMLALAEKGVGELLQHQQEALAT

Sequences:

>Translated_238_residues
MRPSGRRADELRPISIQRQFTKHAEGSVLIGFGDTRVLCTASVEARVPPWLRGQGRGWVTAEYGMLPRSTHTRSDREAAR
GKQQGRTVEIQRLIGRSLRAAVDLQALGERSVVVDCDVLQADGGTRTAAITGAFVALTDALEGLVRQRALPASPLHGQVA
SVSVGVYRGEPVLDLDYAEDGEAETDMNVVMNDAGAFIELQGTAEGHAFRRDELDRMLALAEKGVGELLQHQQEALAT
>Mature_238_residues
MRPSGRRADELRPISIQRQFTKHAEGSVLIGFGDTRVLCTASVEARVPPWLRGQGRGWVTAEYGMLPRSTHTRSDREAAR
GKQQGRTVEIQRLIGRSLRAAVDLQALGERSVVVDCDVLQADGGTRTAAITGAFVALTDALEGLVRQRALPASPLHGQVA
SVSVGVYRGEPVLDLDYAEDGEAETDMNVVMNDAGAFIELQGTAEGHAFRRDELDRMLALAEKGVGELLQHQQEALAT

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=66.9856459330144, Blast_Score=296, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI71981632, Length=192, Percent_Identity=26.0416666666667, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_HALHL (A1WVN7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002551.1
- ProteinModelPortal:   A1WVN7
- SMR:   A1WVN7
- STRING:   A1WVN7
- GeneID:   4709502
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0973
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A1WVN7
- ProtClustDB:   PRK00173
- BioCyc:   HHAL349124:HHAL_0973-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25753; Mature: 25753

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRRADELRPISIQRQFTKHAEGSVLIGFGDTRVLCTASVEARVPPWLRGQGRGWVT
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEE
AEYGMLPRSTHTRSDREAARGKQQGRTVEIQRLIGRSLRAAVDLQALGERSVVVDCDVLQ
EECCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
ADGGTRTAAITGAFVALTDALEGLVRQRALPASPLHGQVASVSVGVYRGEPVLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEECCCCCC
GEAETDMNVVMNDAGAFIELQGTAEGHAFRRDELDRMLALAEKGVGELLQHQQEALAT
CCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSGRRADELRPISIQRQFTKHAEGSVLIGFGDTRVLCTASVEARVPPWLRGQGRGWVT
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEE
AEYGMLPRSTHTRSDREAARGKQQGRTVEIQRLIGRSLRAAVDLQALGERSVVVDCDVLQ
EECCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
ADGGTRTAAITGAFVALTDALEGLVRQRALPASPLHGQVASVSVGVYRGEPVLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCEEECCCCCC
GEAETDMNVVMNDAGAFIELQGTAEGHAFRRDELDRMLALAEKGVGELLQHQQEALAT
CCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA