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The map label for this gene is hyfB [H]

Identifier: 121997602

GI number: 121997602

Start: 893768

End: 895444

Strand: Reverse

Name: hyfB [H]

Synonym: Hhal_0811

Alternate gene names: 121997602

Gene position: 895444-893768 (Counterclockwise)

Preceding gene: 121997603

Following gene: 121997596

Centisome position: 33.43

GC content: 77.52

Gene sequence:

>1677_bases
GTGAGCATCGATCCGGCCGTCGGCCTGCTCGTGCCCGTGCTGATGCCGCTGCTGGCGGTGGCGCTGCTCGGTGGCCGTGC
CCGTGCCCTGGGGGCCGGGCTGCTGGTGGTCGCGCCGCTGACGGCGCTGGCCGCCCTGGCCCTGGGCGGCGAGGTGCTGC
ACCTGCCGTGGCTGCTGCTCGATACCCGCCTGGCGGCGGATCCCATCGGCCAGGCGGTGCTGCTGTTCGCCGCCCCGGTG
GCGGCGGCGGCCCTGCTCTTCGCGGTGGCCGGACAGCGGCACACCGGAGCCACGCCGGGGCTGCTCACCGCCCTGGCGGC
CACCGTCGCCGCGCTGATGGGGGTGCTGCTCGCCGCCGATCTGGCCACCTTCTACTTCGCCTACGCCGCCATGACCCTGG
CCGCCTACCCGCTGGTGGTCCACTTCCGCACCCCGGAGGCCTTCCGCGCCGGGCGGGTCTACCTGGGCATGGCGGTGCTG
GCCGAGGCCGCGGTCCTGGCCGCCGTGCTGCTGATTGCCGCCGAGGGCGGCAACGCCGCGCTCACCGAGGTGCCGGCGCT
GGTCGCCGAGCACGCATACCGCGGCGTGCTGGTGGCGCTGATCGGGGTGGGCTTTGCGGTGAAGGCCGGCGTCGTGCCCC
TGCACGCCTGGCTGGCGCTGGCCCACCCGGCGGCGCCGGTCCCGGCCAGTGCGCTGCTCTCGGCCCTGCTGGTCAAGGCC
GCCCTGGTGGCCTGGTGGCGCCTGCTGCCCATCGGCGAGGTGGCCCTGCCGGCGGCCGGCGGGCTGCTGGTGGCGGCGGG
GCTGGCCAGCAGCCTCTACGCCAGCGTGGTGGGCGTGACCCAGCGCCGGGCCAAGACGGTGCTGGCCTACTCCACGGTGA
GCCAGATGGGGCTGATGACCGCCTTCACCGGCACCGCACTGCTCCAGCCGGCGCTGGCCGGGGTGGCCCTCACCGGCGTG
GCGCTGTTCGCCCTGCACCACGGCCTGGCCAAGGGGGCGCTGTTCCTCGGTTGTGGGCTCCACGGCCGCCACCGCGGCGC
GTACCTGCTGCTGCCGGCGCTGGCCCTGGTGGGGGTGCCGGCCACCAGCGGCGCCCTGGCCAAGGCCGCCGGCAAGCAGC
TCAGCACCGAGGCCGTGGGGGCGCCGGCGGAGCCGCTGCTGCTGCTCGCCTCGGCGCTGACCGCGGTGCTCATGGTCCGT
TTCCTGCTGCTGGCCTGGCGCGGCGACGGGGCGCGGGATCCGCTGGCCCAGCGCGGGGCGGTGCTCGCCCTGGTGGCGCT
GTCGCTGAGCCTGCCGTGGCTGCTGGCCGCCGGGGTGGCCCCGGACTGGGTGGGCTACGCCTGGCGGCCGGCGGTGCTGC
TCGACGCGCTCCTGCCGGCGCTCGCCGGCGGGGCAGTGGCGGCGGTGCTCTTCGGCCTGGCCGGCGCCGAGCGCCTGCCG
CGGATCCCGGAGGGCGACTGGATCAAGCCGGCCCTGCTTGCCGGCGTGCGCCTGGGCCGCACCAGCCGCCGCGCCTGGCG
CCGGCTGCCGGCGCGCCCGCCGGCGCCTTCGCTGGCGGTGGTCGCCCGCCTCGGCGCAGCGGTGGAGGGGCGGCTCATCG
CCCTGGCCATGGCCGGGGCGCTGTTCCTGCTGCTGGTGCTGGGCATCGCCCTGCTGGGCGTGCTGGCGGGGGGCTAG

Upstream 100 bases:

>100_bases
TGTTCACCGCCGCGGCGGCGCTGCTCGCCGGCGTCCTGGCGGCGGCGGAGTTCTCGCCGCTGAGCTGGGCTGTGCGCATC
GTCGAACAGGAGTACCTGCC

Downstream 100 bases:

>100_bases
AAGCGCAGCCCCACGCCGGCGTTGAGCATGAACGCCCGCGGCACGGAGTCCACCGTCTCCGTGCTCATGGCCGCATCCAG
GCTCAGGACGCGGAAGAGCG

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSIDPAVGLLVPVLMPLLAVALLGGRARALGAGLLVVAPLTALAALALGGEVLHLPWLLLDTRLAADPIGQAVLLFAAPV
AAAALLFAVAGQRHTGATPGLLTALAATVAALMGVLLAADLATFYFAYAAMTLAAYPLVVHFRTPEAFRAGRVYLGMAVL
AEAAVLAAVLLIAAEGGNAALTEVPALVAEHAYRGVLVALIGVGFAVKAGVVPLHAWLALAHPAAPVPASALLSALLVKA
ALVAWWRLLPIGEVALPAAGGLLVAAGLASSLYASVVGVTQRRAKTVLAYSTVSQMGLMTAFTGTALLQPALAGVALTGV
ALFALHHGLAKGALFLGCGLHGRHRGAYLLLPALALVGVPATSGALAKAAGKQLSTEAVGAPAEPLLLLASALTAVLMVR
FLLLAWRGDGARDPLAQRGAVLALVALSLSLPWLLAAGVAPDWVGYAWRPAVLLDALLPALAGGAVAAVLFGLAGAERLP
RIPEGDWIKPALLAGVRLGRTSRRAWRRLPARPPAPSLAVVARLGAAVEGRLIALAMAGALFLLLVLGIALLGVLAGG

Sequences:

>Translated_558_residues
MSIDPAVGLLVPVLMPLLAVALLGGRARALGAGLLVVAPLTALAALALGGEVLHLPWLLLDTRLAADPIGQAVLLFAAPV
AAAALLFAVAGQRHTGATPGLLTALAATVAALMGVLLAADLATFYFAYAAMTLAAYPLVVHFRTPEAFRAGRVYLGMAVL
AEAAVLAAVLLIAAEGGNAALTEVPALVAEHAYRGVLVALIGVGFAVKAGVVPLHAWLALAHPAAPVPASALLSALLVKA
ALVAWWRLLPIGEVALPAAGGLLVAAGLASSLYASVVGVTQRRAKTVLAYSTVSQMGLMTAFTGTALLQPALAGVALTGV
ALFALHHGLAKGALFLGCGLHGRHRGAYLLLPALALVGVPATSGALAKAAGKQLSTEAVGAPAEPLLLLASALTAVLMVR
FLLLAWRGDGARDPLAQRGAVLALVALSLSLPWLLAAGVAPDWVGYAWRPAVLLDALLPALAGGAVAAVLFGLAGAERLP
RIPEGDWIKPALLAGVRLGRTSRRAWRRLPARPPAPSLAVVARLGAAVEGRLIALAMAGALFLLLVLGIALLGVLAGG
>Mature_557_residues
SIDPAVGLLVPVLMPLLAVALLGGRARALGAGLLVVAPLTALAALALGGEVLHLPWLLLDTRLAADPIGQAVLLFAAPVA
AAALLFAVAGQRHTGATPGLLTALAATVAALMGVLLAADLATFYFAYAAMTLAAYPLVVHFRTPEAFRAGRVYLGMAVLA
EAAVLAAVLLIAAEGGNAALTEVPALVAEHAYRGVLVALIGVGFAVKAGVVPLHAWLALAHPAAPVPASALLSALLVKAA
LVAWWRLLPIGEVALPAAGGLLVAAGLASSLYASVVGVTQRRAKTVLAYSTVSQMGLMTAFTGTALLQPALAGVALTGVA
LFALHHGLAKGALFLGCGLHGRHRGAYLLLPALALVGVPATSGALAKAAGKQLSTEAVGAPAEPLLLLASALTAVLMVRF
LLLAWRGDGARDPLAQRGAVLALVALSLSLPWLLAAGVAPDWVGYAWRPAVLLDALLPALAGGAVAAVLFGLAGAERLPR
IPEGDWIKPALLAGVRLGRTSRRAWRRLPARPPAPSLAVVARLGAAVEGRLIALAMAGALFLLLVLGIALLGVLAGG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=239, Percent_Identity=31.3807531380753, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788831, Length=139, Percent_Identity=38.1294964028777, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56102; Mature: 55971

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDPAVGLLVPVLMPLLAVALLGGRARALGAGLLVVAPLTALAALALGGEVLHLPWLLL
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DTRLAADPIGQAVLLFAAPVAAAALLFAVAGQRHTGATPGLLTALAATVAALMGVLLAAD
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LATFYFAYAAMTLAAYPLVVHFRTPEAFRAGRVYLGMAVLAEAAVLAAVLLIAAEGGNAA
HHHHHHHHHHHHHHHHHHEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LTEVPALVAEHAYRGVLVALIGVGFAVKAGVVPLHAWLALAHPAAPVPASALLSALLVKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALVAWWRLLPIGEVALPAAGGLLVAAGLASSLYASVVGVTQRRAKTVLAYSTVSQMGLMT
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTGTALLQPALAGVALTGVALFALHHGLAKGALFLGCGLHGRHRGAYLLLPALALVGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHCCC
ATSGALAKAAGKQLSTEAVGAPAEPLLLLASALTAVLMVRFLLLAWRGDGARDPLAQRGA
CCCCHHHHHHCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
VLALVALSLSLPWLLAAGVAPDWVGYAWRPAVLLDALLPALAGGAVAAVLFGLAGAERLP
HHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCC
RIPEGDWIKPALLAGVRLGRTSRRAWRRLPARPPAPSLAVVARLGAAVEGRLIALAMAGA
CCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LFLLLVLGIALLGVLAGG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIDPAVGLLVPVLMPLLAVALLGGRARALGAGLLVVAPLTALAALALGGEVLHLPWLLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DTRLAADPIGQAVLLFAAPVAAAALLFAVAGQRHTGATPGLLTALAATVAALMGVLLAAD
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LATFYFAYAAMTLAAYPLVVHFRTPEAFRAGRVYLGMAVLAEAAVLAAVLLIAAEGGNAA
HHHHHHHHHHHHHHHHHHEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LTEVPALVAEHAYRGVLVALIGVGFAVKAGVVPLHAWLALAHPAAPVPASALLSALLVKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALVAWWRLLPIGEVALPAAGGLLVAAGLASSLYASVVGVTQRRAKTVLAYSTVSQMGLMT
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTGTALLQPALAGVALTGVALFALHHGLAKGALFLGCGLHGRHRGAYLLLPALALVGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHCCC
ATSGALAKAAGKQLSTEAVGAPAEPLLLLASALTAVLMVRFLLLAWRGDGARDPLAQRGA
CCCCHHHHHHCCHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
VLALVALSLSLPWLLAAGVAPDWVGYAWRPAVLLDALLPALAGGAVAAVLFGLAGAERLP
HHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCC
RIPEGDWIKPALLAGVRLGRTSRRAWRRLPARPPAPSLAVVARLGAAVEGRLIALAMAGA
CCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LFLLLVLGIALLGVLAGG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]