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The map label for this gene is rnt [H]

Identifier: 121997525

GI number: 121997525

Start: 813982

End: 814662

Strand: Reverse

Name: rnt [H]

Synonym: Hhal_0728

Alternate gene names: 121997525

Gene position: 814662-813982 (Counterclockwise)

Preceding gene: 121997526

Following gene: 121997524

Centisome position: 30.42

GC content: 68.28

Gene sequence:

>681_bases
ATGACCGAGCAGAACCCCGCGCAGTCGTCCGCCGCGGACGAGCGTATTCAGAGCACCGAGATCCCCCCCCTGCGCACCCG
TTTTCGGGCCTTCCTGCCGGTGGTGGTGGATGTGGAGACGGGCGGGCTGCAGGCCGAGACCGATGCCCTGCTGCAGATCG
CGGCGGTGATCCTGCGCGCCGAGGAGGGCACCGGCCGGCTCTATCCGGCGGAGACGCACACCTGTCATGTCCAGCCTTTC
GAGGGGGCACGCATCGACCCCAAGGCGCTGGAGCTCAACGGCATCGACCCCGAGCACCCACTGCGCATGGCGGTGCCCGA
GCGCGACGCCCTGGGCAAGATGTTCAACCCGGTCCGGCAGGAGTTGCGTAACACCGGCTGCAATCGGGCGGTGCTGGTCG
GCCACAACGCCTTCTTCGATCTGGCCTTCCTCAACGCCGCGGTGACGCGGACCGGCTTCAAGCGCAATCCCTTCCACCCC
TTCTCCAGTTTCGACACCGCCACCCTGGGCGGGCTGGCTTACGGGCAGACGGTGCTGGCCAAGGCGGTGAAGGCCGCCGG
CATCGAGTGGGACGCCAAGGAGGCCCACTCGGCGATCTACGACGCCGAGAAGACCGCCGAGCTCTTCTGCGCCATCGTCA
ACTGCTGGGACGCGCACTGCGGCCGTCCGTCCAGCTTCTAG

Upstream 100 bases:

>100_bases
ACGCCGCCGGGGCGGGCGCCAGCGGCTGAGGGCCGGGCGGCTTGCTCGCGCTCACGGGGCGCCGTAGCATGCGGCACCCG
AATCCAGTTACGGCAGCCCC

Downstream 100 bases:

>100_bases
CCGCCGGGATTCCAAGGGGTTGCGGTGCTTGCGCCGGGTCCCGTCGGCGGTTAGGTTAGACGTTTTTTCCCGGCTTCTAG
AGCCGGTTCCATGCGGGTAT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTEQNPAQSSAADERIQSTEIPPLRTRFRAFLPVVVDVETGGLQAETDALLQIAAVILRAEEGTGRLYPAETHTCHVQPF
EGARIDPKALELNGIDPEHPLRMAVPERDALGKMFNPVRQELRNTGCNRAVLVGHNAFFDLAFLNAAVTRTGFKRNPFHP
FSSFDTATLGGLAYGQTVLAKAVKAAGIEWDAKEAHSAIYDAEKTAELFCAIVNCWDAHCGRPSSF

Sequences:

>Translated_226_residues
MTEQNPAQSSAADERIQSTEIPPLRTRFRAFLPVVVDVETGGLQAETDALLQIAAVILRAEEGTGRLYPAETHTCHVQPF
EGARIDPKALELNGIDPEHPLRMAVPERDALGKMFNPVRQELRNTGCNRAVLVGHNAFFDLAFLNAAVTRTGFKRNPFHP
FSSFDTATLGGLAYGQTVLAKAVKAAGIEWDAKEAHSAIYDAEKTAELFCAIVNCWDAHCGRPSSF
>Mature_225_residues
TEQNPAQSSAADERIQSTEIPPLRTRFRAFLPVVVDVETGGLQAETDALLQIAAVILRAEEGTGRLYPAETHTCHVQPFE
GARIDPKALELNGIDPEHPLRMAVPERDALGKMFNPVRQELRNTGCNRAVLVGHNAFFDLAFLNAAVTRTGFKRNPFHPF
SSFDTATLGGLAYGQTVLAKAVKAAGIEWDAKEAHSAIYDAEKTAELFCAIVNCWDAHCGRPSSF

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=208, Percent_Identity=53.8461538461538, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24675; Mature: 24544

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQNPAQSSAADERIQSTEIPPLRTRFRAFLPVVVDVETGGLQAETDALLQIAAVILRA
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHC
EEGTGRLYPAETHTCHVQPFEGARIDPKALELNGIDPEHPLRMAVPERDALGKMFNPVRQ
CCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHH
ELRNTGCNRAVLVGHNAFFDLAFLNAAVTRTGFKRNPFHPFSSFDTATLGGLAYGQTVLA
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KAVKAAGIEWDAKEAHSAIYDAEKTAELFCAIVNCWDAHCGRPSSF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQNPAQSSAADERIQSTEIPPLRTRFRAFLPVVVDVETGGLQAETDALLQIAAVILRA
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHC
EEGTGRLYPAETHTCHVQPFEGARIDPKALELNGIDPEHPLRMAVPERDALGKMFNPVRQ
CCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHH
ELRNTGCNRAVLVGHNAFFDLAFLNAAVTRTGFKRNPFHPFSSFDTATLGGLAYGQTVLA
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KAVKAAGIEWDAKEAHSAIYDAEKTAELFCAIVNCWDAHCGRPSSF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA