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The map label for this gene is panB

Identifier: 121997472

GI number: 121997472

Start: 757919

End: 758746

Strand: Reverse

Name: panB

Synonym: Hhal_0675

Alternate gene names: 121997472

Gene position: 758746-757919 (Counterclockwise)

Preceding gene: 121997473

Following gene: 121997471

Centisome position: 28.33

GC content: 71.5

Gene sequence:

>828_bases
ATGTCGAGCGGTGATCGCAAGCACGCCTCCGGCGGGGCCGTGACGGTCTCCCGCATGCGCGCGATGAAGCGCGAGGGCCG
GCCCCTGGCCTGCCTGACGGCCTACGACTACGGCTTTGCCGCGGCCTGTGAGCGCGCCGGCGTCGACCTGCTGCTCATCG
GCGACTCTCTCGGTATGGTGGTCCAGGGTCATGAGACCACCCTGCCGGTGACGGTGGACGACATGGTCTACCACACCCGC
TGCGTGGCCCGGGCCTGTCGCCGGGCACTGGTGGTGGCTGATCTGCCGTTCATGAGCCACACCGGTGTGGAGCAGGGCCT
GCACAACGCCGGGCGGTTGATGAAGGAGGGCGGGGCGCAGATGGTCAAGCTCGAGGGCGGCGCCGAGCAGGCGGCGCTGG
TCGAGCGCCTGGCCCAGAACGGCATCCCGGTCTGCGGCCACCTCGGGCTGAAGCCACAGCAGGTGCACAAGATCGGCGGC
TACCGGGTGCAGGGCCGCGAGCAGGCCGACGCCGAGCGCATGGAGGCAGACGCGTTGGCGCTGGAGGCGGCCGGGGCCGA
TCTGCTCATCCTCGAGTGCGTGCCCGCCGTCCTGGCGCAACAGCTGAGTGAGCGGCTGACTGTCCCGGTGGTGGGCATCG
GCGCCGGCGGCGGTTGCGACGGACAGATCCTGGTACTCCACGACGTGCTCGGGGTGACCGAGCAGCCGCCGCGCTTTGCG
CGGGCCTTCGGGGCCGAGGGCGGCGGTGTGCACGCGGCGCTGGAGGCCTACGTGGCGGCGGTACGGGCCGGAACCTTCCC
AGGACCAGAGCACGGGTTCGAGGCTTGA

Upstream 100 bases:

>100_bases
GCTGGCCTGAGGACTGGACGCGGCGCGGCCATGGGTACTTCAATCGGACGCCAGTGGCGCTGTACTCTGTCCCACCTTTC
TCAAGCCGAGGTTGACCCCC

Downstream 100 bases:

>100_bases
GGCAGGTCACCGAGCGCAACGCCATGCGGGAGCTGTCGCGCGCCTGGCGGGCGCGCGGCGAGCGCATCGGGCTGGTGCCG
ACGATGGGCAACCTCCACCG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSSGDRKHASGGAVTVSRMRAMKREGRPLACLTAYDYGFAAACERAGVDLLLIGDSLGMVVQGHETTLPVTVDDMVYHTR
CVARACRRALVVADLPFMSHTGVEQGLHNAGRLMKEGGAQMVKLEGGAEQAALVERLAQNGIPVCGHLGLKPQQVHKIGG
YRVQGREQADAERMEADALALEAAGADLLILECVPAVLAQQLSERLTVPVVGIGAGGGCDGQILVLHDVLGVTEQPPRFA
RAFGAEGGGVHAALEAYVAAVRAGTFPGPEHGFEA

Sequences:

>Translated_275_residues
MSSGDRKHASGGAVTVSRMRAMKREGRPLACLTAYDYGFAAACERAGVDLLLIGDSLGMVVQGHETTLPVTVDDMVYHTR
CVARACRRALVVADLPFMSHTGVEQGLHNAGRLMKEGGAQMVKLEGGAEQAALVERLAQNGIPVCGHLGLKPQQVHKIGG
YRVQGREQADAERMEADALALEAAGADLLILECVPAVLAQQLSERLTVPVVGIGAGGGCDGQILVLHDVLGVTEQPPRFA
RAFGAEGGGVHAALEAYVAAVRAGTFPGPEHGFEA
>Mature_274_residues
SSGDRKHASGGAVTVSRMRAMKREGRPLACLTAYDYGFAAACERAGVDLLLIGDSLGMVVQGHETTLPVTVDDMVYHTRC
VARACRRALVVADLPFMSHTGVEQGLHNAGRLMKEGGAQMVKLEGGAEQAALVERLAQNGIPVCGHLGLKPQQVHKIGGY
RVQGREQADAERMEADALALEAAGADLLILECVPAVLAQQLSERLTVPVVGIGAGGGCDGQILVLHDVLGVTEQPPRFAR
AFGAEGGGVHAALEAYVAAVRAGTFPGPEHGFEA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=261, Percent_Identity=50.5747126436782, Blast_Score=269, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319653, Length=262, Percent_Identity=36.6412213740458, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_HALHL (A1WUU5)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002259.1
- ProteinModelPortal:   A1WUU5
- SMR:   A1WUU5
- STRING:   A1WUU5
- GeneID:   4710246
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0675
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   A1WUU5
- ProtClustDB:   PRK00311
- BioCyc:   HHAL349124:HHAL_0675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28722; Mature: 28591

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: ACT_SITE 192-192 BINDING 94-94 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGDRKHASGGAVTVSRMRAMKREGRPLACLTAYDYGFAAACERAGVDLLLIGDSLGMV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCEE
VQGHETTLPVTVDDMVYHTRCVARACRRALVVADLPFMSHTGVEQGLHNAGRLMKEGGAQ
EECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCE
MVKLEGGAEQAALVERLAQNGIPVCGHLGLKPQQVHKIGGYRVQGREQADAERMEADALA
EEEECCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHH
LEAAGADLLILECVPAVLAQQLSERLTVPVVGIGAGGGCDGQILVLHDVLGVTEQPPRFA
HHHCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEHHHCCCCCCHHHH
RAFGAEGGGVHAALEAYVAAVRAGTFPGPEHGFEA
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSGDRKHASGGAVTVSRMRAMKREGRPLACLTAYDYGFAAACERAGVDLLLIGDSLGMV
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCEE
VQGHETTLPVTVDDMVYHTRCVARACRRALVVADLPFMSHTGVEQGLHNAGRLMKEGGAQ
EECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCE
MVKLEGGAEQAALVERLAQNGIPVCGHLGLKPQQVHKIGGYRVQGREQADAERMEADALA
EEEECCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCEEECCCCHHHHHHHHHHHHH
LEAAGADLLILECVPAVLAQQLSERLTVPVVGIGAGGGCDGQILVLHDVLGVTEQPPRFA
HHHCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEHHHCCCCCCHHHH
RAFGAEGGGVHAALEAYVAAVRAGTFPGPEHGFEA
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA