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The map label for this gene is tilS [H]

Identifier: 121611837

GI number: 121611837

Start: 5458758

End: 5459684

Strand: Direct

Name: tilS [H]

Synonym: Veis_4939

Alternate gene names: 121611837

Gene position: 5458758-5459684 (Clockwise)

Preceding gene: 121611836

Following gene: 121611838

Centisome position: 98.06

GC content: 68.93

Gene sequence:

>927_bases
ATGCAGGCTTTCGCGCCGGCGCTGCCGCTGGCGGTGGGGCTCAGTGGCGGCGCCGATTCGAGCGCCCTGCTGCTGGCCTG
CGCGCGGCGCTGGCCCGGCCAGGTGCAGGCCCTGCATGTGCACCACGGCCTGCAGGCCGCAGCCGATGGCTTCGAGCAGC
ATTGCGCCGCCCTGTGCCAGCGCTTGCAGGTGCCGCTGATGGTGCAGCACCTGCGCGCAAGCCCGGCCCCCGGCCAGAGT
CCCGAAGACGCTGCACGCCAGGCCCGATACCAAGCGTTGGCTGCGCTGGCGCAAGCAAATCAAACCCATGATGCTATTAA
ATCAATAGTCATCGGACAACATGCGGACGATCAGGCCGAGACCGTGCTGCTGGCCCTTTTGCGCGGCGCGGGGGTGGCGG
GCTTGGCCGCCATGCCTGCCCGTTGGGAGATGGCGGGTCTGACCTGGCACCGCCCTTTGCTGCAGGTGACGGCTGCCGAT
GTGCGCCAATGGCTGCGCGCCCAGGGGCAAACCTGGGTGGAAGACCCGAGCAACGCCGACCAGCGCCTGACCCGCAATCG
CATCCGGGCGCAGCTGATGCCTGCGCTCGATGCAGCCTTCCCGACTTTCCGCGACACCTTTGCCCGGTCGGCCGCCAACG
CCGCGCAGGCCGCCCAATTGCTGGAGACGCTGGCGCAGCAGGACCAGGCCCAGGTCGGCAGGCCGCCGCAGATCGGGCCC
TTGCAAGGACTGAGCCGTGCGCGCCAGGCGAATGTGCTGCGCCACTGGCTGCGCAACGCCCACCACACCACACCGGCTGC
GGCCCAACTCCATGAACTGCTGGACCAGATCGAGGCCTGCACCACGCGCGGCCACCGCATCCACATCAAGGTCGGGCGCG
GCTTTGTCGTGCGCTGCGGGCCGCAACTCGATTGGTACAATTCATAG

Upstream 100 bases:

>100_bases
GACCTGCTCGAACGCCGCCATGAGCGCCTGCAAAGCTATGGGCGCTTCAACGACACCAAGGCCGAGGCGCGCTAGATTGA
CGCAAGCCTTCGCTGCCGCC

Downstream 100 bases:

>100_bases
CGTCTTGCTTGGGGAGGAGCAGTTCATCTGCTGCAAGCATGTCTGCTGCGCCTTCCCCTGCCTGTCTCCCCTCAACGCAC
GAAAGGCAGGAACCACGGCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MQAFAPALPLAVGLSGGADSSALLLACARRWPGQVQALHVHHGLQAAADGFEQHCAALCQRLQVPLMVQHLRASPAPGQS
PEDAARQARYQALAALAQANQTHDAIKSIVIGQHADDQAETVLLALLRGAGVAGLAAMPARWEMAGLTWHRPLLQVTAAD
VRQWLRAQGQTWVEDPSNADQRLTRNRIRAQLMPALDAAFPTFRDTFARSAANAAQAAQLLETLAQQDQAQVGRPPQIGP
LQGLSRARQANVLRHWLRNAHHTTPAAAQLHELLDQIEACTTRGHRIHIKVGRGFVVRCGPQLDWYNS

Sequences:

>Translated_308_residues
MQAFAPALPLAVGLSGGADSSALLLACARRWPGQVQALHVHHGLQAAADGFEQHCAALCQRLQVPLMVQHLRASPAPGQS
PEDAARQARYQALAALAQANQTHDAIKSIVIGQHADDQAETVLLALLRGAGVAGLAAMPARWEMAGLTWHRPLLQVTAAD
VRQWLRAQGQTWVEDPSNADQRLTRNRIRAQLMPALDAAFPTFRDTFARSAANAAQAAQLLETLAQQDQAQVGRPPQIGP
LQGLSRARQANVLRHWLRNAHHTTPAAAQLHELLDQIEACTTRGHRIHIKVGRGFVVRCGPQLDWYNS
>Mature_308_residues
MQAFAPALPLAVGLSGGADSSALLLACARRWPGQVQALHVHHGLQAAADGFEQHCAALCQRLQVPLMVQHLRASPAPGQS
PEDAARQARYQALAALAQANQTHDAIKSIVIGQHADDQAETVLLALLRGAGVAGLAAMPARWEMAGLTWHRPLLQVTAAD
VRQWLRAQGQTWVEDPSNADQRLTRNRIRAQLMPALDAAFPTFRDTFARSAANAAQAAQLLETLAQQDQAQVGRPPQIGP
LQGLSRARQANVLRHWLRNAHHTTPAAAQLHELLDQIEACTTRGHRIHIKVGRGFVVRCGPQLDWYNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=307, Percent_Identity=38.1107491856677, Blast_Score=150, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 33389; Mature: 33389

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFAPALPLAVGLSGGADSSALLLACARRWPGQVQALHVHHGLQAAADGFEQHCAALCQ
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
RLQVPLMVQHLRASPAPGQSPEDAARQARYQALAALAQANQTHDAIKSIVIGQHADDQAE
HCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
TVLLALLRGAGVAGLAAMPARWEMAGLTWHRPLLQVTAADVRQWLRAQGQTWVEDPSNAD
HHHHHHHHCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
QRLTRNRIRAQLMPALDAAFPTFRDTFARSAANAAQAAQLLETLAQQDQAQVGRPPQIGP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LQGLSRARQANVLRHWLRNAHHTTPAAAQLHELLDQIEACTTRGHRIHIKVGRGFVVRCG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEC
PQLDWYNS
CCCCCCCC
>Mature Secondary Structure
MQAFAPALPLAVGLSGGADSSALLLACARRWPGQVQALHVHHGLQAAADGFEQHCAALCQ
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
RLQVPLMVQHLRASPAPGQSPEDAARQARYQALAALAQANQTHDAIKSIVIGQHADDQAE
HCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHH
TVLLALLRGAGVAGLAAMPARWEMAGLTWHRPLLQVTAADVRQWLRAQGQTWVEDPSNAD
HHHHHHHHCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
QRLTRNRIRAQLMPALDAAFPTFRDTFARSAANAAQAAQLLETLAQQDQAQVGRPPQIGP
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
LQGLSRARQANVLRHWLRNAHHTTPAAAQLHELLDQIEACTTRGHRIHIKVGRGFVVRCG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEC
PQLDWYNS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA