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The map label for this gene is lexA

Identifier: 121611811

GI number: 121611811

Start: 5430664

End: 5431338

Strand: Direct

Name: lexA

Synonym: Veis_4912

Alternate gene names: 121611811

Gene position: 5430664-5431338 (Clockwise)

Preceding gene: 121611799

Following gene: 121611812

Centisome position: 97.56

GC content: 65.63

Gene sequence:

>675_bases
ATGCTCGACAACCCGAAACTCACCGCCCGCCAGCAACAAGTCCTGGACCTGATCCAGAACACCATGGCACGCACCGGCGC
CCCTCCTACCCGCGCAGAAATCGCAGCCGAACTGGGCTTCAAGTCAGCCAACGCCGCCGAAGAGCATCTGCAAGCCCTGG
CCCGCAAAGGCGCGATCGAACTGGTCAGCGGCACCTCACGCGGCATCCGCCTGCATAGCGAAACGCTGCGCTCCATCCAT
GCCGCCCGCTCCGGCCCATCCGGCGCAGGCAACCCCGGTTCGTCGCCATGGGTGCTGCCGCTGATCGGGCGCGTGGCAGC
CGGCTCGCCCATTCTCGCGCAAGAGCATGTAGAGCAGACCTACAGCGTGGAAAACGGCCTGTTCCAGCACAAGCCGGACT
ACCTGCTCAAAGTGCGCGGCATGTCGATGCGCGACGCCGGCATCATCGACGGCGACCTGCTCGCCGTGCAAGCCACCCGT
GAGGCGCGCAACGGCCAAATCATCGTCGCCCGCCTGGGGGACGATGTCACCGTCAAGCGCCTGCGCCGCACCGCCAGCAC
CATCGAACTGCTGCCCGAAAACCCCGATTACCCGGTGATCGTGGTCCAGCCCGGCGAGCCTTTCGAAATCGAAGGCCTGG
CCGTCGGGCTGATCCGCAACACCATGCTGATGTAA

Upstream 100 bases:

>100_bases
CATGTTTCATCCCCTGTGTCCGAAAAACTGGTTAAAAATACAGCTACTGGATTGAAAAACAGTTGTACGCCACTGTCACC
GACACCACCGGAGGCCCCCC

Downstream 100 bases:

>100_bases
ACCCAAGGCAAGGCCACACCGCTGCCTTAAGCGCTATCAGCATGAAAGTGTAGATAGCCGTAAGAATCAGGACGAAATCC
GATAGTTCGATGCCAAAGAC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLDNPKLTARQQQVLDLIQNTMARTGAPPTRAEIAAELGFKSANAAEEHLQALARKGAIELVSGTSRGIRLHSETLRSIH
AARSGPSGAGNPGSSPWVLPLIGRVAAGSPILAQEHVEQTYSVENGLFQHKPDYLLKVRGMSMRDAGIIDGDLLAVQATR
EARNGQIIVARLGDDVTVKRLRRTASTIELLPENPDYPVIVVQPGEPFEIEGLAVGLIRNTMLM

Sequences:

>Translated_224_residues
MLDNPKLTARQQQVLDLIQNTMARTGAPPTRAEIAAELGFKSANAAEEHLQALARKGAIELVSGTSRGIRLHSETLRSIH
AARSGPSGAGNPGSSPWVLPLIGRVAAGSPILAQEHVEQTYSVENGLFQHKPDYLLKVRGMSMRDAGIIDGDLLAVQATR
EARNGQIIVARLGDDVTVKRLRRTASTIELLPENPDYPVIVVQPGEPFEIEGLAVGLIRNTMLM
>Mature_224_residues
MLDNPKLTARQQQVLDLIQNTMARTGAPPTRAEIAAELGFKSANAAEEHLQALARKGAIELVSGTSRGIRLHSETLRSIH
AARSGPSGAGNPGSSPWVLPLIGRVAAGSPILAQEHVEQTYSVENGLFQHKPDYLLKVRGMSMRDAGIIDGDLLAVQATR
EARNGQIIVARLGDDVTVKRLRRTASTIELLPENPDYPVIVVQPGEPFEIEGLAVGLIRNTMLM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=215, Percent_Identity=55.8139534883721, Blast_Score=233, Evalue=6e-63,
Organism=Escherichia coli, GI1787431, Length=123, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_VEREI (A1WSJ3)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999618.1
- ProteinModelPortal:   A1WSJ3
- STRING:   A1WSJ3
- MEROPS:   S24.001
- GeneID:   4694597
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4912
- NMPDR:   fig|391735.5.peg.4512
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   A1WSJ3
- ProtClustDB:   PRK00215
- BioCyc:   VEIS391735:VEIS_4912-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 24120; Mature: 24120

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: ACT_SITE 142-142 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNPKLTARQQQVLDLIQNTMARTGAPPTRAEIAAELGFKSANAAEEHLQALARKGAIE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
LVSGTSRGIRLHSETLRSIHAARSGPSGAGNPGSSPWVLPLIGRVAAGSPILAQEHVEQT
EECCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
YSVENGLFQHKPDYLLKVRGMSMRDAGIIDGDLLAVQATREARNGQIIVARLGDDVTVKR
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHH
LRRTASTIELLPENPDYPVIVVQPGEPFEIEGLAVGLIRNTMLM
HHHHHHHHEECCCCCCCCEEEECCCCCEEECCEEHHHHHHHHCC
>Mature Secondary Structure
MLDNPKLTARQQQVLDLIQNTMARTGAPPTRAEIAAELGFKSANAAEEHLQALARKGAIE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
LVSGTSRGIRLHSETLRSIHAARSGPSGAGNPGSSPWVLPLIGRVAAGSPILAQEHVEQT
EECCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
YSVENGLFQHKPDYLLKVRGMSMRDAGIIDGDLLAVQATREARNGQIIVARLGDDVTVKR
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHH
LRRTASTIELLPENPDYPVIVVQPGEPFEIEGLAVGLIRNTMLM
HHHHHHHHEECCCCCCCCEEEECCCCCEEECCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA