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The map label for this gene is hisF [H]

Identifier: 121611761

GI number: 121611761

Start: 5374149

End: 5374838

Strand: Direct

Name: hisF [H]

Synonym: Veis_4860

Alternate gene names: 121611761

Gene position: 5374149-5374838 (Clockwise)

Preceding gene: 121611750

Following gene: 121611762

Centisome position: 96.54

GC content: 66.09

Gene sequence:

>690_bases
ATGGCTGCGCGCAGCCTACGACATCTGATCGGTGACGCGACACTAGGCTATTACAGCCAAGGCGCCGACGAGTTGGTCTT
CATGGACATCGTGGCCAGCCTGTATCAGCGCAACAACCTGGCCGGGATCATCGAACGCGCGGCCGATCAGATATATGTGC
CGATCACGGTGGGCGGAGGCATCCGGTCACTGCACGACGTGCGCACCATGCTTTGCTCCGGCGCCGACAAGGTGGCCATC
AACACCGCAGCCGTGGCCCGGCCCGGGCTGATTGCCGAAGTCTCCGAGCGCTTCGGCTCCCAGTGCATGGTGCTGTCCGT
CGAAGCCAAGCGCAAATCCGGCGGCGGCTGGGAGGCTTTCGTCGACAACGGCCGCGAGCATACCGGGCTGGATGTCGTCG
CGTGGGTCCGGCAGGCCGTGGCCCTGGGGGCGGGCGAGATCATGCTGACCTCCGTCGACCAGGAAGGCACGCGCAAAGGT
TTCGACGTCGACCTGGTGCGCACTGTGGCCGGCTGTGTCACGGTGCCCATCATTGCCAGCGGCGGTTATGGCCGGCCCTC
GGACCTGCAACAGGCGGCCGAGGCCGGTGCTTCGGGTTTGGCCATCGCTGATGCGCTGCATTGGCAGCGCGCCACCATTG
CCGAGTTGAAGGCCCATGCGCGCGCAGCCGGTTTGGAGGTTCGCCCGTGA

Upstream 100 bases:

>100_bases
ATCTGTCGGTCTGCGCTGGCCATCGAAGCGCATCGCGGCGTTGCATCGCTTGCCAATACGCTCGGTATTGGCTGCGCGAT
GCGCCTTGCGCTGCGCTCCG

Downstream 100 bases:

>100_bases
GCGCTGCCCGGCCGCCCGAAGGCGCTCACACCGTAGCCGAAGGCGAAGGTACTCCAGCGAACGCCCCTGCAAGCGCCCCT
GCAAGTGCTCCCGTGGGTGC

Product: histidine biosynthesis

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MAARSLRHLIGDATLGYYSQGADELVFMDIVASLYQRNNLAGIIERAADQIYVPITVGGGIRSLHDVRTMLCSGADKVAI
NTAAVARPGLIAEVSERFGSQCMVLSVEAKRKSGGGWEAFVDNGREHTGLDVVAWVRQAVALGAGEIMLTSVDQEGTRKG
FDVDLVRTVAGCVTVPIIASGGYGRPSDLQQAAEAGASGLAIADALHWQRATIAELKAHARAAGLEVRP

Sequences:

>Translated_229_residues
MAARSLRHLIGDATLGYYSQGADELVFMDIVASLYQRNNLAGIIERAADQIYVPITVGGGIRSLHDVRTMLCSGADKVAI
NTAAVARPGLIAEVSERFGSQCMVLSVEAKRKSGGGWEAFVDNGREHTGLDVVAWVRQAVALGAGEIMLTSVDQEGTRKG
FDVDLVRTVAGCVTVPIIASGGYGRPSDLQQAAEAGASGLAIADALHWQRATIAELKAHARAAGLEVRP
>Mature_228_residues
AARSLRHLIGDATLGYYSQGADELVFMDIVASLYQRNNLAGIIERAADQIYVPITVGGGIRSLHDVRTMLCSGADKVAIN
TAAVARPGLIAEVSERFGSQCMVLSVEAKRKSGGGWEAFVDNGREHTGLDVVAWVRQAVALGAGEIMLTSVDQEGTRKGF
DVDLVRTVAGCVTVPIIASGGYGRPSDLQQAAEAGASGLAIADALHWQRATIAELKAHARAAGLEVRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=218, Percent_Identity=37.6146788990826, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082028, Length=202, Percent_Identity=27.2277227722772, Blast_Score=77, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=265, Percent_Identity=30.5660377358491, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 24099; Mature: 23968

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARSLRHLIGDATLGYYSQGADELVFMDIVASLYQRNNLAGIIERAADQIYVPITVGGG
CCHHHHHHHHCCCHHCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCC
IRSLHDVRTMLCSGADKVAINTAAVARPGLIAEVSERFGSQCMVLSVEAKRKSGGGWEAF
HHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHH
VDNGREHTGLDVVAWVRQAVALGAGEIMLTSVDQEGTRKGFDVDLVRTVAGCVTVPIIAS
HCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEC
GGYGRPSDLQQAAEAGASGLAIADALHWQRATIAELKAHARAAGLEVRP
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AARSLRHLIGDATLGYYSQGADELVFMDIVASLYQRNNLAGIIERAADQIYVPITVGGG
CHHHHHHHHCCCHHCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCC
IRSLHDVRTMLCSGADKVAINTAAVARPGLIAEVSERFGSQCMVLSVEAKRKSGGGWEAF
HHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHH
VDNGREHTGLDVVAWVRQAVALGAGEIMLTSVDQEGTRKGFDVDLVRTVAGCVTVPIIAS
HCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEC
GGYGRPSDLQQAAEAGASGLAIADALHWQRATIAELKAHARAAGLEVRP
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]