Click here to switch to the map view.

The map label for this gene is hisH1 [H]

Identifier: 121611762

GI number: 121611762

Start: 5374931

End: 5375743

Strand: Direct

Name: hisH1 [H]

Synonym: Veis_4861

Alternate gene names: 121611762

Gene position: 5374931-5375743 (Clockwise)

Preceding gene: 121611761

Following gene: 121611763

Centisome position: 96.55

GC content: 67.9

Gene sequence:

>813_bases
GTGGGTGCGCGGTCGGTCACCGTCATCGATTACGGAATCGGCAACCTGCTCAACGTAGTCCGCGCCTTGCAGCACTGCGG
TGCCAGCGTCCGGGTGGTGCAAGACGCCGCCGGGCTGCACGCCATGCCCGAGCGTCTGGTGCTCCCGGGCGTCGGCGCCT
TCATCGGCGGCATGGTGGAGATGCGCCAGCGCGGCCTGGACGACCTGATGCGCCGCTTCGCCGCCACCGGCCGGCCGCTG
CTAGTGTCGCGTCACGGATCAGATGTCGTAGGCTGCGCGCAGCCATCGGAGCGCAGCGCAAGGCGCATCGCGCAGCCAAT
ACCGAGCGTATTGGCAAGCGATGCAACGCCGCGATGCGCTCCGATGGCCAGCGCGGACCGACAGATGATCAGTGACGCGA
CACTAGACATTTGCGTCGGGGCGCAGATGCTGTTCGATGCCGGCCAGGAGTTTGGCGAGCACCCCGGGCTGGGTTTGCTT
GCCGGCCGGGTGCAGCCGGTTCCCGAGGCCACGCCGTCCGGCGGCAGGCACCGCATTCCGCATATCGGTTGGAGCGCCCT
TGTCCGCCCGCCCGCCCGGGCGACCTGGGCGCATTCGCCGCTGGCGCAAATGCCCGAAGGGCAGAGCATGTACTTCGTGC
ATTCCTTCGCCCCGGTTCCTGCCGACGAGGCGCACCGCCTAGCCGATACTTTCTATGACGGTGTGCGCATCTGTGCCGCA
GTCGGTCGGGACAACATCTGGGGCTGCCAGTTTCATCCGGAGCGCAGCGCCGAGCACGGCCTTGGCGTGTTGCGCTCTTT
CCTGGCTCTGTGA

Upstream 100 bases:

>100_bases
GCCCGTGAGCGCTGCCCGGCCGCCCGAAGGCGCTCACACCGTAGCCGAAGGCGAAGGTACTCCAGCGAACGCCCCTGCAA
GCGCCCCTGCAAGTGCTCCC

Downstream 100 bases:

>100_bases
CGGCGTATGCACTTCTTGCGCTCCCCCTTGGTGCTCTTTGTCGCCGACCTGGCGCTGGCGCTGCTGGCCTGGTGGCTGGC
CTTCTGGCTGCGTTTCAACC

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MGARSVTVIDYGIGNLLNVVRALQHCGASVRVVQDAAGLHAMPERLVLPGVGAFIGGMVEMRQRGLDDLMRRFAATGRPL
LVSRHGSDVVGCAQPSERSARRIAQPIPSVLASDATPRCAPMASADRQMISDATLDICVGAQMLFDAGQEFGEHPGLGLL
AGRVQPVPEATPSGGRHRIPHIGWSALVRPPARATWAHSPLAQMPEGQSMYFVHSFAPVPADEAHRLADTFYDGVRICAA
VGRDNIWGCQFHPERSAEHGLGVLRSFLAL

Sequences:

>Translated_270_residues
MGARSVTVIDYGIGNLLNVVRALQHCGASVRVVQDAAGLHAMPERLVLPGVGAFIGGMVEMRQRGLDDLMRRFAATGRPL
LVSRHGSDVVGCAQPSERSARRIAQPIPSVLASDATPRCAPMASADRQMISDATLDICVGAQMLFDAGQEFGEHPGLGLL
AGRVQPVPEATPSGGRHRIPHIGWSALVRPPARATWAHSPLAQMPEGQSMYFVHSFAPVPADEAHRLADTFYDGVRICAA
VGRDNIWGCQFHPERSAEHGLGVLRSFLAL
>Mature_269_residues
GARSVTVIDYGIGNLLNVVRALQHCGASVRVVQDAAGLHAMPERLVLPGVGAFIGGMVEMRQRGLDDLMRRFAATGRPLL
VSRHGSDVVGCAQPSERSARRIAQPIPSVLASDATPRCAPMASADRQMISDATLDICVGAQMLFDAGQEFGEHPGLGLLA
GRVQPVPEATPSGGRHRIPHIGWSALVRPPARATWAHSPLAQMPEGQSMYFVHSFAPVPADEAHRLADTFYDGVRICAAV
GRDNIWGCQFHPERSAEHGLGVLRSFLAL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=266, Percent_Identity=28.1954887218045, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 28861; Mature: 28729

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARSVTVIDYGIGNLLNVVRALQHCGASVRVVQDAAGLHAMPERLVLPGVGAFIGGMVE
CCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEHHHHHCCHHCCHHHHCCCHHHHHHHHHH
MRQRGLDDLMRRFAATGRPLLVSRHGSDVVGCAQPSERSARRIAQPIPSVLASDATPRCA
HHHCCHHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PMASADRQMISDATLDICVGAQMLFDAGQEFGEHPGLGLLAGRVQPVPEATPSGGRHRIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
HIGWSALVRPPARATWAHSPLAQMPEGQSMYFVHSFAPVPADEAHRLADTFYDGVRICAA
CCCHHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VGRDNIWGCQFHPERSAEHGLGVLRSFLAL
HCCCCEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GARSVTVIDYGIGNLLNVVRALQHCGASVRVVQDAAGLHAMPERLVLPGVGAFIGGMVE
CCCEEEEEECCHHHHHHHHHHHHHCCCCEEEHHHHHCCHHCCHHHHCCCHHHHHHHHHH
MRQRGLDDLMRRFAATGRPLLVSRHGSDVVGCAQPSERSARRIAQPIPSVLASDATPRCA
HHHCCHHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PMASADRQMISDATLDICVGAQMLFDAGQEFGEHPGLGLLAGRVQPVPEATPSGGRHRIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
HIGWSALVRPPARATWAHSPLAQMPEGQSMYFVHSFAPVPADEAHRLADTFYDGVRICAA
CCCHHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VGRDNIWGCQFHPERSAEHGLGVLRSFLAL
HCCCCEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA