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The map label for this gene is metK

Identifier: 121611746

GI number: 121611746

Start: 5356732

End: 5357943

Strand: Direct

Name: metK

Synonym: Veis_4844

Alternate gene names: 121611746

Gene position: 5356732-5357943 (Clockwise)

Preceding gene: 121611742

Following gene: 121611747

Centisome position: 96.23

GC content: 66.34

Gene sequence:

>1212_bases
ATGGCGAACGACTTTCTCTTCACCTCGGAATCGGTCTCCGAAGGCCACCCCGACAAGGTGGCCGACCAAATCTCGGACGC
GATTCTCGATGCCCTCTTGCGGCAAGACCCGCGCAGCCGCGTTGCCGCCGAGACATTGACCAACACCGGCCTGGTGGTGC
TGGCCGGCGAGATCAGCACCAACGCCCATGTGGACTACATACAGGTGGCGCGCGACACCATCCGGCGCATCGGCTATGAC
GACACCGACTACGGCATCGACTACAAGGGCTGCGCCGTGCTCGTGGCCTACGACAAGCAAAGCAACGACATCGCCCAGGG
CGTGGACCATGCCAGCGACGACCATTTGAACACCGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCTGCGACGAAA
CGCCCGAGCTGATGCCCGCGCCGATCTACTACGCGCACCGCCTCGTCGAGCGCCAGGCCCAGTTGCGCAAGGACGGGCGC
CTGCCCTTTTTGCGCCCTGACGCCAAGAGCCAGGTCACGCTGCGCTATGTGGACGGCAAGCCCCACAGCATCGACACCGT
GGTGCTCTCGACCCAGCACCACCCGGACCAATCGGCCACCGCCACCACCATGAAGCAGAGCTTCATAGACGCGGTGATCG
AGGAAATCATCCGGCCCGTGCTGCCCAAAGAATGGCTGCACGACACGCGCTTCCTGATCAACCCCACCGGCCGCTTCGTC
ACCGGCGGCCCGCAGGGCGACTGCGGCCTGACGGGCCGCAAGATCATCGTCGACACCTACGGCGGGGCCTGCCCGCATGG
CGGCGGCGCGTTCAGCGGCAAGGACCCGACCAAGGTCGACCGCTCCGCCGCCTACGCCGCCCGCCATGTGGCCAAGAACG
TCGTGGCCGCCGGTCTGGCGCGCCAATGCCAGATCCAGGTGGCTTACGCGATCGGCGTGGCCGAGCCGATGAACATCACC
GTGTACACCGAGGGCACGGGTGTGATTGCCGACGAGCGCATCGCCGCGCTGATCAAGCAGCATTTCGACCTGCGCCCCCG
GGGCATCATCCAGATGCTCGACCTGCTGCGCCCGATCTACGAAAAAACCGCCGCCTACGGCCACTTCGGCCGCGAAGAGC
CTGAGTTCACCTGGGAGCGGACGGACAAAGCGGCTGCGCTGCGCGCGGCAGCCGGACTGTCACCCGGCGAGCACCACAGA
AAAGAGGGCTGA

Upstream 100 bases:

>100_bases
TTGCAGGGCGACGTTAAAAAACTCTGCTAAAGCGTTCGCCATAGCTCCTACGGGGTCAGGGCAACGCCCAAATGCCGAAC
CACCACAGGAGTTTATTCAA

Downstream 100 bases:

>100_bases
CAGCGCCGCCAGACCATGCGCCAGCGGCCCGCTGCAATGGACTGGCCAGAGACTAGTGTCGCGTCACGGATCAGATGTCG
TAGGCTGCGCGCAGCCATCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MANDFLFTSESVSEGHPDKVADQISDAILDALLRQDPRSRVAAETLTNTGLVVLAGEISTNAHVDYIQVARDTIRRIGYD
DTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
LPFLRPDAKSQVTLRYVDGKPHSIDTVVLSTQHHPDQSATATTMKQSFIDAVIEEIIRPVLPKEWLHDTRFLINPTGRFV
TGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARHVAKNVVAAGLARQCQIQVAYAIGVAEPMNIT
VYTEGTGVIADERIAALIKQHFDLRPRGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWERTDKAAALRAAAGLSPGEHHR
KEG

Sequences:

>Translated_403_residues
MANDFLFTSESVSEGHPDKVADQISDAILDALLRQDPRSRVAAETLTNTGLVVLAGEISTNAHVDYIQVARDTIRRIGYD
DTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
LPFLRPDAKSQVTLRYVDGKPHSIDTVVLSTQHHPDQSATATTMKQSFIDAVIEEIIRPVLPKEWLHDTRFLINPTGRFV
TGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARHVAKNVVAAGLARQCQIQVAYAIGVAEPMNIT
VYTEGTGVIADERIAALIKQHFDLRPRGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWERTDKAAALRAAAGLSPGEHHR
KEG
>Mature_402_residues
ANDFLFTSESVSEGHPDKVADQISDAILDALLRQDPRSRVAAETLTNTGLVVLAGEISTNAHVDYIQVARDTIRRIGYDD
TDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRL
PFLRPDAKSQVTLRYVDGKPHSIDTVVLSTQHHPDQSATATTMKQSFIDAVIEEIIRPVLPKEWLHDTRFLINPTGRFVT
GGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARHVAKNVVAAGLARQCQIQVAYAIGVAEPMNITV
YTEGTGVIADERIAALIKQHFDLRPRGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWERTDKAAALRAAAGLSPGEHHRK
EG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=57.1052631578947, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=55.5263157894737, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=66.5809768637532, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=54.5931758530184, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=54.1450777202073, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=54.0682414698163, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=53.2467532467532, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=51.6417910447761, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=48.5564304461942, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=51.1834319526627, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=51.2987012987013, Blast_Score=284, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=60.7142857142857, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=56.5104166666667, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=56.3636363636364, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=52.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=51.8421052631579, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=51.8421052631579, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=49.3438320209974, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.1428571428571, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_VEREI (A1WSC8)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999553.1
- ProteinModelPortal:   A1WSC8
- SMR:   A1WSC8
- STRING:   A1WSC8
- GeneID:   4694186
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4844
- NMPDR:   fig|391735.5.peg.4448
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A1WSC8
- ProtClustDB:   PRK05250
- BioCyc:   VEIS391735:VEIS_4844-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44032; Mature: 43901

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFLFTSESVSEGHPDKVADQISDAILDALLRQDPRSRVAAETLTNTGLVVLAGEIST
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIRRIGYDDTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCC
GDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRLPFLRPDAKSQVTLRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCC
PHSIDTVVLSTQHHPDQSATATTMKQSFIDAVIEEIIRPVLPKEWLHDTRFLINPTGRFV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCEE
TGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARHVAKNVVAAGLA
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RQCQIQVAYAIGVAEPMNITVYTEGTGVIADERIAALIKQHFDLRPRGIIQMLDLLRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKTAAYGHFGREEPEFTWERTDKAAALRAAAGLSPGEHHRKEG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCC
>Mature Secondary Structure 
ANDFLFTSESVSEGHPDKVADQISDAILDALLRQDPRSRVAAETLTNTGLVVLAGEIST
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIRRIGYDDTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCC
GDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRLPFLRPDAKSQVTLRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCC
PHSIDTVVLSTQHHPDQSATATTMKQSFIDAVIEEIIRPVLPKEWLHDTRFLINPTGRFV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCEE
TGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARHVAKNVVAAGLA
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RQCQIQVAYAIGVAEPMNITVYTEGTGVIADERIAALIKQHFDLRPRGIIQMLDLLRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKTAAYGHFGREEPEFTWERTDKAAALRAAAGLSPGEHHRKEG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA