Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 121611660

GI number: 121611660

Start: 5246114

End: 5247166

Strand: Direct

Name: potD-A [H]

Synonym: Veis_4755

Alternate gene names: 121611660

Gene position: 5246114-5247166 (Clockwise)

Preceding gene: 121611659

Following gene: 121611663

Centisome position: 94.24

GC content: 54.61

Gene sequence:

>1053_bases
ATGCTTGGGAAAATCGTGACCAAACTGGCCGCCCTGGCCATGACAGCCGGTATTTTGCTCTCCGGAGCAGCGCATGCCGA
AGATGCCATCAATTTATACAACTGGTCCAACTACATTCCACCGGACTTGCTGAAGAAATTCGAGAAAGACACCGGCATCA
AAGTGCACCTCGATGTCTACGATTCCAATGAGACATTGCTGGCCAAAATCAAGGCCGGGGCTGCCGGCTATGATGTCATC
GTTCCGACGGATTACATGATAAAGATCATGATCGACGAAAAGCTGCTCACCCGGTTCGAGGCCGGCAAGCTGGCCAACTT
CAAGAACGTCATGCCCCCGCTGGACAAGCCTTCCTTTGACGCAAAACGGGAATACTCCGCGCCCTATATGTGGGGCGTCA
CCGGTTTCACATATGACAGCGCGCGCGTTCCGGGCGGCAAACTCGAAGACAGTTGGCAATCGTTCTTCGAACCCCCCGCC
GCGCTCAAGGGACGGGTAGCGGCGCTGAATGATGCAAACGACATATTTTCCGCCGCATCCCTGTACCTGGGCATAGACCA
ATGTACCGAAGACCCGAAAGCCGGTCAGAGGATTCTGAACGTTCTCGAAAAACAAAAACCCTTTCTGGCCACGTACAACT
CGAATGGAACCATTGAACGCATGCAAGCCGGGGAAGTGATAATGCATCACCAGTGGAACGGTAGCGCCTTCCGCACCAGG
GAAAAGCTCAAGACCGCCGTGTTCGTGATTCCACGCGAAGGAGTGCCTTTCTGGAACGATAATTTTGCGATTCCCGTGGG
CGCAAAAAATCCCGAAGGGGCCAGGAAATTCATCGATTGGATGATGGCTCCAGAGAACATTGCCATCGCCTCCAACTTCA
CTGGCTACAACAATGCCATCAGGGGTGCGGACCAATTCATGAATGCCGCTTTGCGTGCCGACCCCGGCGTGAATCCCGGC
GACGAAATGCGCAAGCGCTTCCGGCCCAACCAGATCTGTAGCCTCAAAGCCATGGAATTGCGCAACAGGATCTGGACGCG
GCTGATGCGCTAG

Upstream 100 bases:

>100_bases
TGGCGGCCTTGCTGCTGATCGCATCCATGGTGTTTGTCGGTCTTTCGTGGTGGATTGGCCAGCGCGACCGCCGTTGATTT
TTGATCCGATGGAGATGAAG

Downstream 100 bases:

>100_bases
TGTCGCGTCACCGATCAGATGTCGCAGGCTGCGCGCAGCCGTCGGAGCGTAGCGCAAGGCGCAGCGCTTGCCCATACCGA
GCTGTATTGGCAAGCGATGC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLGKIVTKLAALAMTAGILLSGAAHAEDAINLYNWSNYIPPDLLKKFEKDTGIKVHLDVYDSNETLLAKIKAGAAGYDVI
VPTDYMIKIMIDEKLLTRFEAGKLANFKNVMPPLDKPSFDAKREYSAPYMWGVTGFTYDSARVPGGKLEDSWQSFFEPPA
ALKGRVAALNDANDIFSAASLYLGIDQCTEDPKAGQRILNVLEKQKPFLATYNSNGTIERMQAGEVIMHHQWNGSAFRTR
EKLKTAVFVIPREGVPFWNDNFAIPVGAKNPEGARKFIDWMMAPENIAIASNFTGYNNAIRGADQFMNAALRADPGVNPG
DEMRKRFRPNQICSLKAMELRNRIWTRLMR

Sequences:

>Translated_350_residues
MLGKIVTKLAALAMTAGILLSGAAHAEDAINLYNWSNYIPPDLLKKFEKDTGIKVHLDVYDSNETLLAKIKAGAAGYDVI
VPTDYMIKIMIDEKLLTRFEAGKLANFKNVMPPLDKPSFDAKREYSAPYMWGVTGFTYDSARVPGGKLEDSWQSFFEPPA
ALKGRVAALNDANDIFSAASLYLGIDQCTEDPKAGQRILNVLEKQKPFLATYNSNGTIERMQAGEVIMHHQWNGSAFRTR
EKLKTAVFVIPREGVPFWNDNFAIPVGAKNPEGARKFIDWMMAPENIAIASNFTGYNNAIRGADQFMNAALRADPGVNPG
DEMRKRFRPNQICSLKAMELRNRIWTRLMR
>Mature_350_residues
MLGKIVTKLAALAMTAGILLSGAAHAEDAINLYNWSNYIPPDLLKKFEKDTGIKVHLDVYDSNETLLAKIKAGAAGYDVI
VPTDYMIKIMIDEKLLTRFEAGKLANFKNVMPPLDKPSFDAKREYSAPYMWGVTGFTYDSARVPGGKLEDSWQSFFEPPA
ALKGRVAALNDANDIFSAASLYLGIDQCTEDPKAGQRILNVLEKQKPFLATYNSNGTIERMQAGEVIMHHQWNGSAFRTR
EKLKTAVFVIPREGVPFWNDNFAIPVGAKNPEGARKFIDWMMAPENIAIASNFTGYNNAIRGADQFMNAALRADPGVNPG
DEMRKRFRPNQICSLKAMELRNRIWTRLMR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=306, Percent_Identity=36.2745098039216, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1787078, Length=355, Percent_Identity=30.1408450704225, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787711, Length=311, Percent_Identity=25.4019292604502, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39091; Mature: 39091

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKIVTKLAALAMTAGILLSGAAHAEDAINLYNWSNYIPPDLLKKFEKDTGIKVHLDVY
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEEE
DSNETLLAKIKAGAAGYDVIVPTDYMIKIMIDEKLLTRFEAGKLANFKNVMPPLDKPSFD
CCCCEEEEEEECCCCCCEEEECCCEEEEEEECHHHHHHHHCCCCCCHHHCCCCCCCCCCC
AKREYSAPYMWGVTGFTYDSARVPGGKLEDSWQSFFEPPAALKGRVAALNDANDIFSAAS
CCCCCCCCEEECCCCEEECCCCCCCCCCHHHHHHHCCCCHHHCCCEEEECCHHHHHHHHH
LYLGIDQCTEDPKAGQRILNVLEKQKPFLATYNSNGTIERMQAGEVIMHHQWNGSAFRTR
HHEEHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEEEECCCCHHHHH
EKLKTAVFVIPREGVPFWNDNFAIPVGAKNPEGARKFIDWMMAPENIAIASNFTGYNNAI
HHHCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RGADQFMNAALRADPGVNPGDEMRKRFRPNQICSLKAMELRNRIWTRLMR
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLGKIVTKLAALAMTAGILLSGAAHAEDAINLYNWSNYIPPDLLKKFEKDTGIKVHLDVY
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCHHHHHHHHHCCCCEEEEEEE
DSNETLLAKIKAGAAGYDVIVPTDYMIKIMIDEKLLTRFEAGKLANFKNVMPPLDKPSFD
CCCCEEEEEEECCCCCCEEEECCCEEEEEEECHHHHHHHHCCCCCCHHHCCCCCCCCCCC
AKREYSAPYMWGVTGFTYDSARVPGGKLEDSWQSFFEPPAALKGRVAALNDANDIFSAAS
CCCCCCCCEEECCCCEEECCCCCCCCCCHHHHHHHCCCCHHHCCCEEEECCHHHHHHHHH
LYLGIDQCTEDPKAGQRILNVLEKQKPFLATYNSNGTIERMQAGEVIMHHQWNGSAFRTR
HHEEHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEEEECCCCHHHHH
EKLKTAVFVIPREGVPFWNDNFAIPVGAKNPEGARKFIDWMMAPENIAIASNFTGYNNAI
HHHCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RGADQFMNAALRADPGVNPGDEMRKRFRPNQICSLKAMELRNRIWTRLMR
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]