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The map label for this gene is potB [H]

Identifier: 121611658

GI number: 121611658

Start: 5244382

End: 5245299

Strand: Direct

Name: potB [H]

Synonym: Veis_4753

Alternate gene names: 121611658

Gene position: 5244382-5245299 (Clockwise)

Preceding gene: 121611657

Following gene: 121611659

Centisome position: 94.21

GC content: 58.82

Gene sequence:

>918_bases
ATGCAGATGCCGCGCATTGCCCCGCCTGCCTTGCGACGCTTCAGCCAGCGCTGGGGACAGTGGCTGCTGCTGGCACCCAC
ATTGGCGACCCTTGGCCTGTTCATGGTGCTGCCGATGCTGTGGGTGGCTGTGATGTCACTGCTCGAAGCCGCCCCCTACG
GCGGCGTGCGTTGGGGGCATTGGACGACGGATGCCTATTTGCGCTTTTTCATGGAGTTGGACCTCGATGGCCATTGGGTC
CTGAATACCGACTACATGGCCATATTCTCCAGATCATTCGGGCTGGCGCTGGCCGTCGTGGTGATTACCCTCGGTGTCGG
TTTTCCGCTGGCTTTGCACATTGCCCTTCAGGAAGAAAGCAAGCGCAACCACATGCTGCTGTGGATTACCATTCCATTTT
GGGTCAACCTGCTGGTGCGCACTTACGCCTGGATATTGTTGCTGCGCAACGGCGGAGTGATCGAACAAAGCCTGAACCTG
GTGGGCTTTCAGACCGGGGGACTCAACATTTTGTACACCCCGCTGGCGACAGGCATCGGTCTGGTCTACGCCTATCTGCC
CTTCATGGTGCTGCCAATTTATACCAGCCTGGAAAAAATGGACTGGCGTCTGTTCGAAGCGGCGCTGGACCTGGGCGCTA
CGCGGTGGATGGCGCTGCGCCGGATCGTCATCCCTTTGGCCATGCCGGGGATTGTCGCGGGCTGCCTGCTGGTATTCATT
CCGGCGCTGGGCGCTTATTACATCCCGGAACTGCTGGGGGGTGGCAAGCAAATGATGATTGGCAGCCTGATCCAGTCGCA
ATTCGGCGTGGCACGCAATTGGCCCTTCGGTTCGGCACTGGCTTTCGCATTGCTTTCGATCGTGCTGGCAGGCATGTTGC
TGTATACGCGCCGGTTCAAAAAGGCTCTGGACACATGA

Upstream 100 bases:

>100_bases
ACGCGCAGGGTGCGGCGCAGGTGTTTGCGGTCGGCGATGCAGTGGGTCTGCGCCTGGATGCAGCCGCATTGCGGCGGCTG
AACGATTGACTGGAACTACC

Downstream 100 bases:

>100_bases
CCCACCGCAACACCGCGCTGTGGCGCTTTCCCGGGCTGGGATTGAACTTCGGCATCGCATTGGTCTTTTTGTACCTGCCG
ATCGTGCTGCTGGTGCTGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQMPRIAPPALRRFSQRWGQWLLLAPTLATLGLFMVLPMLWVAVMSLLEAAPYGGVRWGHWTTDAYLRFFMELDLDGHWV
LNTDYMAIFSRSFGLALAVVVITLGVGFPLALHIALQEESKRNHMLLWITIPFWVNLLVRTYAWILLLRNGGVIEQSLNL
VGFQTGGLNILYTPLATGIGLVYAYLPFMVLPIYTSLEKMDWRLFEAALDLGATRWMALRRIVIPLAMPGIVAGCLLVFI
PALGAYYIPELLGGGKQMMIGSLIQSQFGVARNWPFGSALAFALLSIVLAGMLLYTRRFKKALDT

Sequences:

>Translated_305_residues
MQMPRIAPPALRRFSQRWGQWLLLAPTLATLGLFMVLPMLWVAVMSLLEAAPYGGVRWGHWTTDAYLRFFMELDLDGHWV
LNTDYMAIFSRSFGLALAVVVITLGVGFPLALHIALQEESKRNHMLLWITIPFWVNLLVRTYAWILLLRNGGVIEQSLNL
VGFQTGGLNILYTPLATGIGLVYAYLPFMVLPIYTSLEKMDWRLFEAALDLGATRWMALRRIVIPLAMPGIVAGCLLVFI
PALGAYYIPELLGGGKQMMIGSLIQSQFGVARNWPFGSALAFALLSIVLAGMLLYTRRFKKALDT
>Mature_305_residues
MQMPRIAPPALRRFSQRWGQWLLLAPTLATLGLFMVLPMLWVAVMSLLEAAPYGGVRWGHWTTDAYLRFFMELDLDGHWV
LNTDYMAIFSRSFGLALAVVVITLGVGFPLALHIALQEESKRNHMLLWITIPFWVNLLVRTYAWILLLRNGGVIEQSLNL
VGFQTGGLNILYTPLATGIGLVYAYLPFMVLPIYTSLEKMDWRLFEAALDLGATRWMALRRIVIPLAMPGIVAGCLLVFI
PALGAYYIPELLGGGKQMMIGSLIQSQFGVARNWPFGSALAFALLSIVLAGMLLYTRRFKKALDT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=274, Percent_Identity=38.6861313868613, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=40.4255319148936, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1787713, Length=237, Percent_Identity=31.2236286919831, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787368, Length=69, Percent_Identity=40.5797101449275, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34167; Mature: 34167

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMPRIAPPALRRFSQRWGQWLLLAPTLATLGLFMVLPMLWVAVMSLLEAAPYGGVRWGH
CCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WTTDAYLRFFMELDLDGHWVLNTDYMAIFSRSFGLALAVVVITLGVGFPLALHIALQEES
CCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCC
KRNHMLLWITIPFWVNLLVRTYAWILLLRNGGVIEQSLNLVGFQTGGLNILYTPLATGIG
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEEEEHHHHHHHH
LVYAYLPFMVLPIYTSLEKMDWRLFEAALDLGATRWMALRRIVIPLAMPGIVAGCLLVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PALGAYYIPELLGGGKQMMIGSLIQSQFGVARNWPFGSALAFALLSIVLAGMLLYTRRFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALDT
HHHCC
>Mature Secondary Structure
MQMPRIAPPALRRFSQRWGQWLLLAPTLATLGLFMVLPMLWVAVMSLLEAAPYGGVRWGH
CCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WTTDAYLRFFMELDLDGHWVLNTDYMAIFSRSFGLALAVVVITLGVGFPLALHIALQEES
CCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCC
KRNHMLLWITIPFWVNLLVRTYAWILLLRNGGVIEQSLNLVGFQTGGLNILYTPLATGIG
CCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEEEEHHHHHHHH
LVYAYLPFMVLPIYTSLEKMDWRLFEAALDLGATRWMALRRIVIPLAMPGIVAGCLLVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PALGAYYIPELLGGGKQMMIGSLIQSQFGVARNWPFGSALAFALLSIVLAGMLLYTRRFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALDT
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]