Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 121611596

GI number: 121611596

Start: 5176329

End: 5177168

Strand: Direct

Name: potB [H]

Synonym: Veis_4688

Alternate gene names: 121611596

Gene position: 5176329-5177168 (Clockwise)

Preceding gene: 121611595

Following gene: 121611597

Centisome position: 92.99

GC content: 64.4

Gene sequence:

>840_bases
ATGAACGCGCGCAAAGAGGGCTGGATCTACGTCGGTCCCGCCACCCTGTTCCTCTTCCTGGTGATGCTGGTCCCGCTCGC
GTACGTCGCGTACACGAGCGTGACCAACGCCGGCGGAGACTTCAGCCTGGCTTCCTACCATCGGTTGTTCTCCAGTGACC
TCTTCAAGCGGACACTGGGAACCACCTTCCAGATCAGCATCCTCGCATCGCTGTGCAGCCTCGCGCTGGGCTATCCGATC
GCGCTGCATCTGGCACGGCAGACGCCCCGCATGCGCGCGATCTACATGGTCCTGGTGCTGGTGCCCTTCTGGACCAGCAT
CCTCGTCAAGAGCTACGCCTTCACCGTGCTGCTGGGGCGCGCGGGGCTGGTCAACCAGCTCCTGTCTTTCGTGTCGGGCA
GCACGGTGCAGCTGCCGATGCTCTTCAACCGATTCGGCGTGATGGTCGGCATGACGAACTACCTGATTCCCTTCATCGTC
TTCCCCGTGCTCGCCAGCCTGCTCGCGATCGACAAGTCGCTGTACCGCGCCTCGGAGATCATGGGCGCGAAGCCGCCCCG
CATCTTCTTTCGGATCACCCTGCCGCTGAGCCTGCCAGGCGTCGCAGCGGGCGTGCTAAGCACCACCGTCATGTCGATGG
GCTTCTTCGTCATTCCCGCGCTGCTCGGCGGACGCCAGGACGTGATGATGTCGAACCTCGTCGACTTCTACACCCGCGAG
ACGATGGACTGGAACATGGCGTCGGCCATCGGCGTCATCCTGCTGGGCATCGTCACGCTGGCCGCCATCCTCGCCGACCA
GGCGAAGCGGTTCGGCAGCCGCCCCGGGGTGCCGGCATGA

Upstream 100 bases:

>100_bases
CAGAACAAGAACAACCTGCTGAGCAACGACGCCTGGTGGGCCGACCACTACGACGAACTCACGCTGAAGTTCAAAGAGTG
GGTCCTGAGCTGATCGACCG

Downstream 100 bases:

>100_bases
GCACATCGGCATCGCGCGGCGTGATCGATCGCCAGGGCAACTTCTACGGCGGCGTGCGCAACCTGCTGGCCGTCTTCGTC
TACGCGTTCATCTTGGTACC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNARKEGWIYVGPATLFLFLVMLVPLAYVAYTSVTNAGGDFSLASYHRLFSSDLFKRTLGTTFQISILASLCSLALGYPI
ALHLARQTPRMRAIYMVLVLVPFWTSILVKSYAFTVLLGRAGLVNQLLSFVSGSTVQLPMLFNRFGVMVGMTNYLIPFIV
FPVLASLLAIDKSLYRASEIMGAKPPRIFFRITLPLSLPGVAAGVLSTTVMSMGFFVIPALLGGRQDVMMSNLVDFYTRE
TMDWNMASAIGVILLGIVTLAAILADQAKRFGSRPGVPA

Sequences:

>Translated_279_residues
MNARKEGWIYVGPATLFLFLVMLVPLAYVAYTSVTNAGGDFSLASYHRLFSSDLFKRTLGTTFQISILASLCSLALGYPI
ALHLARQTPRMRAIYMVLVLVPFWTSILVKSYAFTVLLGRAGLVNQLLSFVSGSTVQLPMLFNRFGVMVGMTNYLIPFIV
FPVLASLLAIDKSLYRASEIMGAKPPRIFFRITLPLSLPGVAAGVLSTTVMSMGFFVIPALLGGRQDVMMSNLVDFYTRE
TMDWNMASAIGVILLGIVTLAAILADQAKRFGSRPGVPA
>Mature_279_residues
MNARKEGWIYVGPATLFLFLVMLVPLAYVAYTSVTNAGGDFSLASYHRLFSSDLFKRTLGTTFQISILASLCSLALGYPI
ALHLARQTPRMRAIYMVLVLVPFWTSILVKSYAFTVLLGRAGLVNQLLSFVSGSTVQLPMLFNRFGVMVGMTNYLIPFIV
FPVLASLLAIDKSLYRASEIMGAKPPRIFFRITLPLSLPGVAAGVLSTTVMSMGFFVIPALLGGRQDVMMSNLVDFYTRE
TMDWNMASAIGVILLGIVTLAAILADQAKRFGSRPGVPA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=31.5126050420168, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787713, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30441; Mature: 30441

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARKEGWIYVGPATLFLFLVMLVPLAYVAYTSVTNAGGDFSLASYHRLFSSDLFKRTLG
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
TTFQISILASLCSLALGYPIALHLARQTPRMRAIYMVLVLVPFWTSILVKSYAFTVLLGR
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGLVNQLLSFVSGSTVQLPMLFNRFGVMVGMTNYLIPFIVFPVLASLLAIDKSLYRASEI
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGAKPPRIFFRITLPLSLPGVAAGVLSTTVMSMGFFVIPALLGGRQDVMMSNLVDFYTRE
HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TMDWNMASAIGVILLGIVTLAAILADQAKRFGSRPGVPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNARKEGWIYVGPATLFLFLVMLVPLAYVAYTSVTNAGGDFSLASYHRLFSSDLFKRTLG
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
TTFQISILASLCSLALGYPIALHLARQTPRMRAIYMVLVLVPFWTSILVKSYAFTVLLGR
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGLVNQLLSFVSGSTVQLPMLFNRFGVMVGMTNYLIPFIVFPVLASLLAIDKSLYRASEI
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGAKPPRIFFRITLPLSLPGVAAGVLSTTVMSMGFFVIPALLGGRQDVMMSNLVDFYTRE
HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TMDWNMASAIGVILLGIVTLAAILADQAKRFGSRPGVPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]