Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 121611595

GI number: 121611595

Start: 5175251

End: 5176321

Strand: Direct

Name: potD-A [H]

Synonym: Veis_4687

Alternate gene names: 121611595

Gene position: 5175251-5176321 (Clockwise)

Preceding gene: 121611594

Following gene: 121611596

Centisome position: 92.97

GC content: 64.33

Gene sequence:

>1071_bases
ATGTCTTCTCCTATTCCTACGAATCACGGTCGTCGCAAGTTCCTGCAGACCACGGGCGCAGCGGCCGCATCGGCAGCGAT
CGGTCTGCCGATGCTGGCCACGCCGCGCCGTGCATCCGCCGCCGAGAAGCTGGTCGTCGTCAGCTGGGGCGGCAACTACC
GCACCGCCGTGGAAGAGGCGCTCGTGCGCCCCTTCGAGAAGGAATTCGGCGTCGCCGTTACGCTGGTCGACACGCCGGAC
CTCGCTAAAGTCAAGGCGCAGGTCATGACCAAGAACGTCGAATGGGACGTCTTCGACGCGGTCGGCCCGATGGCCATGAC
CGGATCGAAGGCCGGCTACTGGGAGACGCTGGACCCGGCGCTCTTCGACCGCAACGATCTGGTCGCACCGATGACCAAGG
AAGCCGTGCCCTTCTACGGCTTCACCGGCGGCATCTTCTGGGATGCCAAGAAGTTCCCGGAAGGCAAGGCACCGCAAACC
TTCGCCGACTACTTTGACGTGACGAAATTCCCCGGCAAGCGCACGCTGCGCAACCGCGCGAGCGAGACGCTCGAGATCGC
GCTGTTGGCCGACGGCGTGGCGCCGGACAAGATGTACCCGCTCGACGTTCCGCGTGCCTTCAAGGCCCTTGAGCGCATCA
AGCCGCACATCGCGAAGTGGGTCGACCAGACGCCGCAGACCACGTCGCTGGTCGAAACCGGCGAAGTGGACTACAGCTAC
ACCTACGCGACCCGCGCCAAGGCATCGCAGGAGTCGGGCAAGCCGACGCAGTTCTCCTTCAAGCAGAACCTGATCGGCCT
CGAGTACCTGGTCGTGCTCAAAGGCGCGCCGAACAAGGCGAACGCGATGAAGTACGTCCAGTTCGCGCTGCGTCCCGACC
GCCAGGCCGCGCTGATGGACCTGCACGGCAATACGCCGGCGAGCAAGAAGGCGCGCGCGCTGATGAAGCCCGGGGTGAGC
AAGTGGCTGTCCGACCCGCAGAACAAGAACAACCTGCTGAGCAACGACGCCTGGTGGGCCGACCACTACGACGAACTCAC
GCTGAAGTTCAAAGAGTGGGTCCTGAGCTGA

Upstream 100 bases:

>100_bases
GCAGGCCAGCGCGAAGATCAAGGAGCGCATCGCCCAGGTGCTCCCGCCGCTGCGCGATCGCGAATAACCCGTTTCCGTCA
ACCCTCTCAAGGTGGTCATG

Downstream 100 bases:

>100_bases
TCGACCGATGAACGCGCGCAAAGAGGGCTGGATCTACGTCGGTCCCGCCACCCTGTTCCTCTTCCTGGTGATGCTGGTCC
CGCTCGCGTACGTCGCGTAC

Product: spermidine/putrescine transporter ABC periplasmic ligand-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSPIPTNHGRRKFLQTTGAAAASAAIGLPMLATPRRASAAEKLVVVSWGGNYRTAVEEALVRPFEKEFGVAVTLVDTPD
LAKVKAQVMTKNVEWDVFDAVGPMAMTGSKAGYWETLDPALFDRNDLVAPMTKEAVPFYGFTGGIFWDAKKFPEGKAPQT
FADYFDVTKFPGKRTLRNRASETLEIALLADGVAPDKMYPLDVPRAFKALERIKPHIAKWVDQTPQTTSLVETGEVDYSY
TYATRAKASQESGKPTQFSFKQNLIGLEYLVVLKGAPNKANAMKYVQFALRPDRQAALMDLHGNTPASKKARALMKPGVS
KWLSDPQNKNNLLSNDAWWADHYDELTLKFKEWVLS

Sequences:

>Translated_356_residues
MSSPIPTNHGRRKFLQTTGAAAASAAIGLPMLATPRRASAAEKLVVVSWGGNYRTAVEEALVRPFEKEFGVAVTLVDTPD
LAKVKAQVMTKNVEWDVFDAVGPMAMTGSKAGYWETLDPALFDRNDLVAPMTKEAVPFYGFTGGIFWDAKKFPEGKAPQT
FADYFDVTKFPGKRTLRNRASETLEIALLADGVAPDKMYPLDVPRAFKALERIKPHIAKWVDQTPQTTSLVETGEVDYSY
TYATRAKASQESGKPTQFSFKQNLIGLEYLVVLKGAPNKANAMKYVQFALRPDRQAALMDLHGNTPASKKARALMKPGVS
KWLSDPQNKNNLLSNDAWWADHYDELTLKFKEWVLS
>Mature_355_residues
SSPIPTNHGRRKFLQTTGAAAASAAIGLPMLATPRRASAAEKLVVVSWGGNYRTAVEEALVRPFEKEFGVAVTLVDTPDL
AKVKAQVMTKNVEWDVFDAVGPMAMTGSKAGYWETLDPALFDRNDLVAPMTKEAVPFYGFTGGIFWDAKKFPEGKAPQTF
ADYFDVTKFPGKRTLRNRASETLEIALLADGVAPDKMYPLDVPRAFKALERIKPHIAKWVDQTPQTTSLVETGEVDYSYT
YATRAKASQESGKPTQFSFKQNLIGLEYLVVLKGAPNKANAMKYVQFALRPDRQAALMDLHGNTPASKKARALMKPGVSK
WLSDPQNKNNLLSNDAWWADHYDELTLKFKEWVLS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39442; Mature: 39311

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPIPTNHGRRKFLQTTGAAAASAAIGLPMLATPRRASAAEKLVVVSWGGNYRTAVEEA
CCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
LVRPFEKEFGVAVTLVDTPDLAKVKAQVMTKNVEWDVFDAVGPMAMTGSKAGYWETLDPA
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHCCCCEEECCCCCCCCCCCCHH
LFDRNDLVAPMTKEAVPFYGFTGGIFWDAKKFPEGKAPQTFADYFDVTKFPGKRTLRNRA
HHCCCCCCCCCHHCCCCCCCCCCCEEECHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHC
SETLEIALLADGVAPDKMYPLDVPRAFKALERIKPHIAKWVDQTPQTTSLVETGEVDYSY
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCEEE
TYATRAKASQESGKPTQFSFKQNLIGLEYLVVLKGAPNKANAMKYVQFALRPDRQAALMD
EEHHHHHHHHCCCCCCEEHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
LHGNTPASKKARALMKPGVSKWLSDPQNKNNLLSNDAWWADHYDELTLKFKEWVLS
ECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPIPTNHGRRKFLQTTGAAAASAAIGLPMLATPRRASAAEKLVVVSWGGNYRTAVEEA
CCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
LVRPFEKEFGVAVTLVDTPDLAKVKAQVMTKNVEWDVFDAVGPMAMTGSKAGYWETLDPA
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCEEHHHCCCCEEECCCCCCCCCCCCHH
LFDRNDLVAPMTKEAVPFYGFTGGIFWDAKKFPEGKAPQTFADYFDVTKFPGKRTLRNRA
HHCCCCCCCCCHHCCCCCCCCCCCEEECHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHC
SETLEIALLADGVAPDKMYPLDVPRAFKALERIKPHIAKWVDQTPQTTSLVETGEVDYSY
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCEEE
TYATRAKASQESGKPTQFSFKQNLIGLEYLVVLKGAPNKANAMKYVQFALRPDRQAALMD
EEHHHHHHHHCCCCCCEEHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
LHGNTPASKKARALMKPGVSKWLSDPQNKNNLLSNDAWWADHYDELTLKFKEWVLS
ECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]