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The map label for this gene is alkJ [H]

Identifier: 121611429

GI number: 121611429

Start: 4999702

End: 5001330

Strand: Direct

Name: alkJ [H]

Synonym: Veis_4519

Alternate gene names: 121611429

Gene position: 4999702-5001330 (Clockwise)

Preceding gene: 121611428

Following gene: 121611430

Centisome position: 89.81

GC content: 63.54

Gene sequence:

>1629_bases
ATGCGTTCCGTAGAAACTGATTACGTAGTCATCGGCGCCGGTTCAGCCGGCTGCGTACTTGCCAACCGTTTGTCGGCCGA
CCCGGTCAATCATGTCGTCGTGCTTGAGGCCGGGGGCGTGGACCGCAACATCTGGATCCACATACCCATCGGCTACGGAA
AGACTTTCTTCGACAAAGAGATCAACTGGATGTTCAAGACCGAGCCCGAGCCGGCCTTGGGTGGGCGCGCCATCGATCAG
CCACGCGGCCGTGTGCTTGGTGGATCCAGTTCCATCAACGGCCTGCTCTACGTGCGCGGCCAAGCTCAGGACTACGACGG
ATGGGCCGCGTTGGGCAACTTTGGCTGGGGTTTTCCGGACGTGCTGCCGTTCTTCAAGCGCGCCGAAGATCAACAGCGCG
GTGCCGATGCCTGGCATGGGGTTGGTGGCCCGTTGTCCGTGAGCGATCTGCCCGAGCCTCATCCGATCGCAGATGCCTTC
ATCGCTTCTGCGGAGGCCAACGGCGTGCCACGCAATCCGGATTTCAATGGGAGCAGGCAAGAAGGCGTGGGCTACTTTCA
GGCGACGGCGCGGCGTGGCCTGCGCAGGAGCACGGCGCGAGCGTATCTGCATCCGGTGATGACGCGCTCCAACCTGCAGG
TACAGACCGGCGCGCAGGTCGGCCGCATTCTGCTGGAAGGTGCCGGCGATGCATTGCGTGCCGTCGGCGTGGCCTATGTC
AAGGACGGGCGCGAACAGCGCGTGATGGCAAGGCGGGAAGTAATTCTGTCGGGCGGTGCGATCCAATCACCGCAGATTCT
GCAACTGTCTGGCGTCGGTCCGGCCGCGCTGCTGCGCCAACACCGGATTGCTGTAGTGCGCGACCTGCCCGGCGTCGGCG
CAAACCTGCAAGACCACATGCAGGGACGCTTGATCTATCAGACCCATGCGCCGATCACGCTCAACGACGACATGATGGGC
ATCGCCGGACGCATCCGGATCGGGCTGCGCTACATGTTGCAGCGCAAGGGCCCGTTGGGTTGGTGGGCGGGGGTGGCAGG
CGGCTTTGCGCGCACCCGCCCGGATCTGGACCGTCCCGACATCCAGTTCCATCTCTACCCTTTTAGCACCGACCGCAAAG
ACAAGCCTGCGCTGCACAGGTTCTCGGCCTTCACGCTCACCGTATGCCAACTGCGACCGTACTCGCGCGGCTCAGTTCAT
ATCCAGAGCGCAAATCCGCTACAGGCGCCGGCAATCCGGATGAACTACCTCAGCGACCCGCGCGACATCGAGGTGCTGAC
CAGCGGGCTCGTGCTGGCCCGCCAGATCGCAAGCACCGCACCACTGGCCGGCCTCATCAAGACCGAGCGCAGCCCGGGCA
TTGAGGTGACGTCACGTGCCGGCTTGCACAAGTTTCTTCGGGAAAAGGGGATGTCGGTGTACCACCCGGTCGGGACCTGC
CGCATGGGCGCGTCGGCCGACTGTGTGGTGGACGAGCGGCTGCGCGTGCATGGCATCTCGGGCCTGCGCGTGTCTGATGC
GTCCATCATGCCCACCCTCATTTCCGGCAACACCAATGCACCGGCCATCATGATCGGCGAGAAAGCCGCCGACATGATTT
TGACCGACCAATCCGGCAAGGCTGCTTGA

Upstream 100 bases:

>100_bases
TGTGTTGTTCGGTCAAAAGTACCTCGTGCGTGGCTTGCAGACCAGCGGTCTGGCGAACTGAGCGGCACTGCGGTTTTGCT
GATTGACGCTGGAGATTTTT

Downstream 100 bases:

>100_bases
TATGCACACCTATCCTGAACTGGGCTTGTTCATCGACGGACAGTGGCATCTCGCGCAAGGTCGCAATACCGAACCGGTCA
TGGACCCTGCCACCGGTCGA

Product: glucose-methanol-choline oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MRSVETDYVVIGAGSAGCVLANRLSADPVNHVVVLEAGGVDRNIWIHIPIGYGKTFFDKEINWMFKTEPEPALGGRAIDQ
PRGRVLGGSSSINGLLYVRGQAQDYDGWAALGNFGWGFPDVLPFFKRAEDQQRGADAWHGVGGPLSVSDLPEPHPIADAF
IASAEANGVPRNPDFNGSRQEGVGYFQATARRGLRRSTARAYLHPVMTRSNLQVQTGAQVGRILLEGAGDALRAVGVAYV
KDGREQRVMARREVILSGGAIQSPQILQLSGVGPAALLRQHRIAVVRDLPGVGANLQDHMQGRLIYQTHAPITLNDDMMG
IAGRIRIGLRYMLQRKGPLGWWAGVAGGFARTRPDLDRPDIQFHLYPFSTDRKDKPALHRFSAFTLTVCQLRPYSRGSVH
IQSANPLQAPAIRMNYLSDPRDIEVLTSGLVLARQIASTAPLAGLIKTERSPGIEVTSRAGLHKFLREKGMSVYHPVGTC
RMGASADCVVDERLRVHGISGLRVSDASIMPTLISGNTNAPAIMIGEKAADMILTDQSGKAA

Sequences:

>Translated_542_residues
MRSVETDYVVIGAGSAGCVLANRLSADPVNHVVVLEAGGVDRNIWIHIPIGYGKTFFDKEINWMFKTEPEPALGGRAIDQ
PRGRVLGGSSSINGLLYVRGQAQDYDGWAALGNFGWGFPDVLPFFKRAEDQQRGADAWHGVGGPLSVSDLPEPHPIADAF
IASAEANGVPRNPDFNGSRQEGVGYFQATARRGLRRSTARAYLHPVMTRSNLQVQTGAQVGRILLEGAGDALRAVGVAYV
KDGREQRVMARREVILSGGAIQSPQILQLSGVGPAALLRQHRIAVVRDLPGVGANLQDHMQGRLIYQTHAPITLNDDMMG
IAGRIRIGLRYMLQRKGPLGWWAGVAGGFARTRPDLDRPDIQFHLYPFSTDRKDKPALHRFSAFTLTVCQLRPYSRGSVH
IQSANPLQAPAIRMNYLSDPRDIEVLTSGLVLARQIASTAPLAGLIKTERSPGIEVTSRAGLHKFLREKGMSVYHPVGTC
RMGASADCVVDERLRVHGISGLRVSDASIMPTLISGNTNAPAIMIGEKAADMILTDQSGKAA
>Mature_542_residues
MRSVETDYVVIGAGSAGCVLANRLSADPVNHVVVLEAGGVDRNIWIHIPIGYGKTFFDKEINWMFKTEPEPALGGRAIDQ
PRGRVLGGSSSINGLLYVRGQAQDYDGWAALGNFGWGFPDVLPFFKRAEDQQRGADAWHGVGGPLSVSDLPEPHPIADAF
IASAEANGVPRNPDFNGSRQEGVGYFQATARRGLRRSTARAYLHPVMTRSNLQVQTGAQVGRILLEGAGDALRAVGVAYV
KDGREQRVMARREVILSGGAIQSPQILQLSGVGPAALLRQHRIAVVRDLPGVGANLQDHMQGRLIYQTHAPITLNDDMMG
IAGRIRIGLRYMLQRKGPLGWWAGVAGGFARTRPDLDRPDIQFHLYPFSTDRKDKPALHRFSAFTLTVCQLRPYSRGSVH
IQSANPLQAPAIRMNYLSDPRDIEVLTSGLVLARQIASTAPLAGLIKTERSPGIEVTSRAGLHKFLREKGMSVYHPVGTC
RMGASADCVVDERLRVHGISGLRVSDASIMPTLISGNTNAPAIMIGEKAADMILTDQSGKAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=539, Percent_Identity=43.7847866419295, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI1786503, Length=537, Percent_Identity=40.9683426443203, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17532301, Length=548, Percent_Identity=37.043795620438, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24642042, Length=570, Percent_Identity=40.3508771929825, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24642059, Length=565, Percent_Identity=39.1150442477876, Blast_Score=357, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45551458, Length=575, Percent_Identity=39.6521739130435, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=39.5104895104895, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24642048, Length=566, Percent_Identity=37.8091872791519, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24642039, Length=575, Percent_Identity=37.0434782608696, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24642055, Length=571, Percent_Identity=38.0035026269702, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI18859995, Length=574, Percent_Identity=37.6306620209059, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17137792, Length=552, Percent_Identity=37.1376811594203, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24650267, Length=567, Percent_Identity=36.8606701940035, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24642037, Length=586, Percent_Identity=35.3242320819113, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24642035, Length=577, Percent_Identity=36.5684575389948, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859993, Length=575, Percent_Identity=35.304347826087, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24642051, Length=583, Percent_Identity=33.1046312178388, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24642057, Length=579, Percent_Identity=30.7426597582038, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24645930, Length=584, Percent_Identity=28.4246575342466, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 58619; Mature: 58619

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVETDYVVIGAGSAGCVLANRLSADPVNHVVVLEAGGVDRNIWIHIPIGYGKTFFDKE
CCCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCC
INWMFKTEPEPALGGRAIDQPRGRVLGGSSSINGLLYVRGQAQDYDGWAALGNFGWGFPD
CCEEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHH
VLPFFKRAEDQQRGADAWHGVGGPLSVSDLPEPHPIADAFIASAEANGVPRNPDFNGSRQ
HHHHHHHCCHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHH
EGVGYFQATARRGLRRSTARAYLHPVMTRSNLQVQTGAQVGRILLEGAGDALRAVGVAYV
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHEEECCCHHHHHHHHHHH
KDGREQRVMARREVILSGGAIQSPQILQLSGVGPAALLRQHRIAVVRDLPGVGANLQDHM
HCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCHHHHHHHHCEEEEECCCCCCCCHHHHC
QGRLIYQTHAPITLNDDMMGIAGRIRIGLRYMLQRKGPLGWWAGVAGGFARTRPDLDRPD
CCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCC
IQFHLYPFSTDRKDKPALHRFSAFTLTVCQLRPYSRGSVHIQSANPLQAPAIRMNYLSDP
EEEEEEECCCCCCCCCHHHHHHEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCC
RDIEVLTSGLVLARQIASTAPLAGLIKTERSPGIEVTSRAGLHKFLREKGMSVYHPVGTC
CHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCCCC
RMGASADCVVDERLRVHGISGLRVSDASIMPTLISGNTNAPAIMIGEKAADMILTDQSGK
CCCCCCCEEECCCEEEECCCCCEECCCCCCEEEECCCCCCCEEEECCCCCCEEEECCCCC
AA
CC
>Mature Secondary Structure
MRSVETDYVVIGAGSAGCVLANRLSADPVNHVVVLEAGGVDRNIWIHIPIGYGKTFFDKE
CCCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCC
INWMFKTEPEPALGGRAIDQPRGRVLGGSSSINGLLYVRGQAQDYDGWAALGNFGWGFPD
CCEEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHH
VLPFFKRAEDQQRGADAWHGVGGPLSVSDLPEPHPIADAFIASAEANGVPRNPDFNGSRQ
HHHHHHHCCHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHH
EGVGYFQATARRGLRRSTARAYLHPVMTRSNLQVQTGAQVGRILLEGAGDALRAVGVAYV
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHEEECCCHHHHHHHHHHH
KDGREQRVMARREVILSGGAIQSPQILQLSGVGPAALLRQHRIAVVRDLPGVGANLQDHM
HCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCHHHHHHHHCEEEEECCCCCCCCHHHHC
QGRLIYQTHAPITLNDDMMGIAGRIRIGLRYMLQRKGPLGWWAGVAGGFARTRPDLDRPD
CCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCC
IQFHLYPFSTDRKDKPALHRFSAFTLTVCQLRPYSRGSVHIQSANPLQAPAIRMNYLSDP
EEEEEEECCCCCCCCCHHHHHHEEEEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCC
RDIEVLTSGLVLARQIASTAPLAGLIKTERSPGIEVTSRAGLHKFLREKGMSVYHPVGTC
CHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEHHHHHHHHHHHCCCEEECCCCCC
RMGASADCVVDERLRVHGISGLRVSDASIMPTLISGNTNAPAIMIGEKAADMILTDQSGK
CCCCCCCEEECCCEEEECCCCCEECCCCCCEEEECCCCCCCEEEECCCCCCEEEECCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]