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The map label for this gene is hisH [H]

Identifier: 121611290

GI number: 121611290

Start: 4826951

End: 4827619

Strand: Direct

Name: hisH [H]

Synonym: Veis_4376

Alternate gene names: 121611290

Gene position: 4826951-4827619 (Clockwise)

Preceding gene: 121611289

Following gene: 121611291

Centisome position: 86.71

GC content: 66.82

Gene sequence:

>669_bases
ATGAATACGCAAGCAAAAACCGTCGCCGTGGTGGACTACGGCATGGGCAACCTGCGCTCGGTCGCGCAGGCCGTGCAGGC
CGCCGCCCAGGGCACGGGCTGGACCGTCGTGGTCACGCAGCGCCCGGAGGTCGTGCGCGCCGCGCAGCGCGTCGTGCTGC
CCGGCCAGGGCGCCATGCCCGACTGCATGCATGAATTGCGTGCCTCGGGCCTGCAACCGGCGGTGCTCGAAGCCGCCGCC
AGCAAGCCCCTGTTCGGCATCTGCGTGGGCATGCAGATGCTGCTCGAGCACAGTGCCGAGGGCGACACGCCCGGCCTCGG
GTTGATCCGCGGCGTGGTGCGCCGATTCGATCTGAGCGGCCAAACCCAGCCTGACGGCAGCCGCTACAAGGTGCCCCAGA
TGGGTTGGAACCAGGTGCGCCAGATGGCGCACGGCGGCGCCGTCCACCCCTTGTGGGCGGCGGTGCCCGACGACAGCCAT
TTCTACTTCGTGCACAGCTTGTATGCCATCCCGCAATCTGCGGCCCATTGCGCCGGGCAGGCCGACTATGGCGGCTGGTT
TGCCGCCGCGATTGCACGCGATAATATTTTTGCCACGCAATTTCACCCGGAAAAGAGTGCCGAGCACGGCCTGACTCTGT
ACCGCAATTTTCTGCACTGGAACCCCTGA

Upstream 100 bases:

>100_bases
GCATCGCCTCGACCAAAGGCTCTTTGTAAAGTGCTGTGAACCGTGTTGATTTCCGTGATTGAATCCGGGCACTGAGCGCT
TTCGACACACGCTGGCTGCC

Downstream 100 bases:

>100_bases
CCCTTTCAACCTCGTTCGGGCCGAGTTCCGTCTCGCCCTTTTCCACCCGAACCCACCATGCTGCTCATCCCCGCCATCGA
TCTCAAGGACGGTCATTGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNTQAKTVAVVDYGMGNLRSVAQAVQAAAQGTGWTVVVTQRPEVVRAAQRVVLPGQGAMPDCMHELRASGLQPAVLEAAA
SKPLFGICVGMQMLLEHSAEGDTPGLGLIRGVVRRFDLSGQTQPDGSRYKVPQMGWNQVRQMAHGGAVHPLWAAVPDDSH
FYFVHSLYAIPQSAAHCAGQADYGGWFAAAIARDNIFATQFHPEKSAEHGLTLYRNFLHWNP

Sequences:

>Translated_222_residues
MNTQAKTVAVVDYGMGNLRSVAQAVQAAAQGTGWTVVVTQRPEVVRAAQRVVLPGQGAMPDCMHELRASGLQPAVLEAAA
SKPLFGICVGMQMLLEHSAEGDTPGLGLIRGVVRRFDLSGQTQPDGSRYKVPQMGWNQVRQMAHGGAVHPLWAAVPDDSH
FYFVHSLYAIPQSAAHCAGQADYGGWFAAAIARDNIFATQFHPEKSAEHGLTLYRNFLHWNP
>Mature_222_residues
MNTQAKTVAVVDYGMGNLRSVAQAVQAAAQGTGWTVVVTQRPEVVRAAQRVVLPGQGAMPDCMHELRASGLQPAVLEAAA
SKPLFGICVGMQMLLEHSAEGDTPGLGLIRGVVRRFDLSGQTQPDGSRYKVPQMGWNQVRQMAHGGAVHPLWAAVPDDSH
FYFVHSLYAIPQSAAHCAGQADYGGWFAAAIARDNIFATQFHPEKSAEHGLTLYRNFLHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=214, Percent_Identity=35.0467289719626, Blast_Score=111, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319725, Length=227, Percent_Identity=33.0396475770925, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23986; Mature: 23986

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQAKTVAVVDYGMGNLRSVAQAVQAAAQGTGWTVVVTQRPEVVRAAQRVVLPGQGAMP
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEECCCCCCCH
DCMHELRASGLQPAVLEAAASKPLFGICVGMQMLLEHSAEGDTPGLGLIRGVVRRFDLSG
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
QTQPDGSRYKVPQMGWNQVRQMAHGGAVHPLWAAVPDDSHFYFVHSLYAIPQSAAHCAGQ
CCCCCCCEEECCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEHHHHHHHCCHHHHHHCCC
ADYGGWFAAAIARDNIFATQFHPEKSAEHGLTLYRNFLHWNP
CCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTQAKTVAVVDYGMGNLRSVAQAVQAAAQGTGWTVVVTQRPEVVRAAQRVVLPGQGAMP
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEECCCCCCCH
DCMHELRASGLQPAVLEAAASKPLFGICVGMQMLLEHSAEGDTPGLGLIRGVVRRFDLSG
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
QTQPDGSRYKVPQMGWNQVRQMAHGGAVHPLWAAVPDDSHFYFVHSLYAIPQSAAHCAGQ
CCCCCCCEEECCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEHHHHHHHCCHHHHHHCCC
ADYGGWFAAAIARDNIFATQFHPEKSAEHGLTLYRNFLHWNP
CCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA