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The map label for this gene is ispDF [H]

Identifier: 121611274

GI number: 121611274

Start: 4807306

End: 4808049

Strand: Direct

Name: ispDF [H]

Synonym: Veis_4360

Alternate gene names: 121611274

Gene position: 4807306-4808049 (Clockwise)

Preceding gene: 121611272

Following gene: 121611275

Centisome position: 86.36

GC content: 73.25

Gene sequence:

>744_bases
GTGGGCACGCGGGCTGCGGCGGCGGGCGGCGCTGCCAGTTCCTGCCCCGTGTCGGCCCCCTGCCCTGCCCTCGCGCCTGC
GCTGCCCAAGCAGTACCAGTTGCTCGCCGGGCGCCCGATGGTGCTGCACACGCTGGCCGCTTTTGCCGGGGTGGGTCGGC
TGCTGGGCACGCTGGTGGCGGTGGCGCCCGGGGATCATTTTCTGGCGCAGCATGCGCAGCCGGCTTTCTTTGTCGTGCCA
TGCGGCGGCCCCCGGCGTGCCGACACGGTGCTGGGCGGTCTGCGGGCCTTGCTCGCGCGCGGCGCGCAGCCCGATGACTG
GGTGCTGGTGCACGATGCCGCGCGCTGTCTGGTGACCTCCGGGCAGATCGAGGCGCTGATGGAGCAATGCGCCAACGACA
GCGTCGGCGGCTTGCTGGCGCTGCGGCTGACCGATACCCTGAAGGCCGCCAGCGACGGCCCGGGCGGCCTGCGCGTGGCC
GGCACGCTCGAGCGCAGCGACAAATGGCTGGCGCAGACGCCGCAGATGTTTCGCATCGGCCCGCTGCTGGCGGCCATCGA
GCAAGCGGGCGGCGACATTACCGACGAGGCCAGCGCGATGGAGGCGCAGGGCCTGCGGCCGCGCCTGGTGCCCGGCGGGG
CGCAGAACTTCAAGGTGACTTATCCCGATGACTTCGCGCTGGCGGCGGCGGTGCTGGCACAGCGCGTGCCGGGCGGGCAG
CGGGCAGAGTCGCTCGTGCGATGA

Upstream 100 bases:

>100_bases
CGATCCCTTGCCGTCATCTTCCCCGCTCGCCCCTTCTTTGCCGCCGTCTCCATCCCGGTCTGCCCCGGCGCGCTTTTGGG
CGCTGCTGCCCTGCGCCGGG

Downstream 100 bases:

>100_bases
CGACACGGGAGCAGATGATGAATCTCAGAATCGGCGAAGGTTGGGATGTGCACGCCCTGGTGCCCGGCCGCAGGTTGGTC
ATCGGCGGCGTGGAGTTGGA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MGTRAAAAGGAASSCPVSAPCPALAPALPKQYQLLAGRPMVLHTLAAFAGVGRLLGTLVAVAPGDHFLAQHAQPAFFVVP
CGGPRRADTVLGGLRALLARGAQPDDWVLVHDAARCLVTSGQIEALMEQCANDSVGGLLALRLTDTLKAASDGPGGLRVA
GTLERSDKWLAQTPQMFRIGPLLAAIEQAGGDITDEASAMEAQGLRPRLVPGGAQNFKVTYPDDFALAAAVLAQRVPGGQ
RAESLVR

Sequences:

>Translated_247_residues
MGTRAAAAGGAASSCPVSAPCPALAPALPKQYQLLAGRPMVLHTLAAFAGVGRLLGTLVAVAPGDHFLAQHAQPAFFVVP
CGGPRRADTVLGGLRALLARGAQPDDWVLVHDAARCLVTSGQIEALMEQCANDSVGGLLALRLTDTLKAASDGPGGLRVA
GTLERSDKWLAQTPQMFRIGPLLAAIEQAGGDITDEASAMEAQGLRPRLVPGGAQNFKVTYPDDFALAAAVLAQRVPGGQ
RAESLVR
>Mature_246_residues
GTRAAAAGGAASSCPVSAPCPALAPALPKQYQLLAGRPMVLHTLAAFAGVGRLLGTLVAVAPGDHFLAQHAQPAFFVVPC
GGPRRADTVLGGLRALLARGAQPDDWVLVHDAARCLVTSGQIEALMEQCANDSVGGLLALRLTDTLKAASDGPGGLRVAG
TLERSDKWLAQTPQMFRIGPLLAAIEQAGGDITDEASAMEAQGLRPRLVPGGAQNFKVTYPDDFALAAAVLAQRVPGGQR
AESLVR

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=214, Percent_Identity=39.7196261682243, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 25355; Mature: 25224

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRAAAAGGAASSCPVSAPCPALAPALPKQYQLLAGRPMVLHTLAAFAGVGRLLGTLVA
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VAPGDHFLAQHAQPAFFVVPCGGPRRADTVLGGLRALLARGAQPDDWVLVHDAARCLVTS
HCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHCC
GQIEALMEQCANDSVGGLLALRLTDTLKAASDGPGGLRVAGTLERSDKWLAQTPQMFRIG
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCCCEEEEEEHHHCCHHHHCCCCHHHHH
PLLAAIEQAGGDITDEASAMEAQGLRPRLVPGGAQNFKVTYPDDFALAAAVLAQRVPGGQ
HHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCH
RAESLVR
HHHHHCC
>Mature Secondary Structure 
GTRAAAAGGAASSCPVSAPCPALAPALPKQYQLLAGRPMVLHTLAAFAGVGRLLGTLVA
CCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VAPGDHFLAQHAQPAFFVVPCGGPRRADTVLGGLRALLARGAQPDDWVLVHDAARCLVTS
HCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHCC
GQIEALMEQCANDSVGGLLALRLTDTLKAASDGPGGLRVAGTLERSDKWLAQTPQMFRIG
HHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCCCEEEEEEHHHCCHHHHCCCCHHHHH
PLLAAIEQAGGDITDEASAMEAQGLRPRLVPGGAQNFKVTYPDDFALAAAVLAQRVPGGQ
HHHHHHHHHCCCCCCHHHHHHHCCCCCEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCH
RAESLVR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA