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The map label for this gene is ispF

Identifier: 121611275

GI number: 121611275

Start: 4808067

End: 4808618

Strand: Direct

Name: ispF

Synonym: Veis_4361

Alternate gene names: 121611275

Gene position: 4808067-4808618 (Clockwise)

Preceding gene: 121611274

Following gene: 121611279

Centisome position: 86.37

GC content: 71.56

Gene sequence:

>552_bases
ATGAATCTCAGAATCGGCGAAGGTTGGGATGTGCACGCCCTGGTGCCCGGCCGCAGGTTGGTCATCGGCGGCGTGGAGTT
GGAGCACCCGATGGGGTTGGGGTTGCTCGGCCACTCGGATGCCGATGTGCTGCTGCATGCGATCACCGATGCGCTGCTGG
GCGCCGCCGCGTTGGGCGATATCGGCCGCCATTTCCCCGACACCGATGCCGCGTTTCGGGCTGCGGATTCGCGCCTGCTG
CTGGCCGAGGCGGCGCGGCGCGTGCGCGCTGCCGGCTACGAGATCGGCAACATCGACAGCACCGTGGTGGCGCAGGCGCC
GCGCCTGGCGGCGCATATTCCGGCGATGCGCCTGGCCATCGCCCGCGCGCTGGGCGTGGATCAGGGCCAGGTCAACGTGA
AGGCCAAGACGGCCGAGGGCTTGGGGCCGGTGGGGCAGAATCTGGCGATCGAGGCGCGGGCCGTGGCACTGATCTGCGCC
ACGCCGGGGGCCGGCGCAATCGGGCCGGCGGCTCAGCGCGTGTCGAGCGCCTGGTCGGGCTCGGGCGCCTGA

Upstream 100 bases:

>100_bases
ATCCCGATGACTTCGCGCTGGCGGCGGCGGTGCTGGCACAGCGCGTGCCGGGCGGGCAGCGGGCAGAGTCGCTCGTGCGA
TGACGACACGGGAGCAGATG

Downstream 100 bases:

>100_bases
AGCGGCGGCAGTTGGCGCTTGATCGCAGGCCGCAGCAGGCGCTGCTTGAGGCCCGTCGCCTTGGGCGGCACGATGGCGCG
CTGCAATCGCAAAAGGACCA

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNLRIGEGWDVHALVPGRRLVIGGVELEHPMGLGLLGHSDADVLLHAITDALLGAAALGDIGRHFPDTDAAFRAADSRLL
LAEAARRVRAAGYEIGNIDSTVVAQAPRLAAHIPAMRLAIARALGVDQGQVNVKAKTAEGLGPVGQNLAIEARAVALICA
TPGAGAIGPAAQRVSSAWSGSGA

Sequences:

>Translated_183_residues
MNLRIGEGWDVHALVPGRRLVIGGVELEHPMGLGLLGHSDADVLLHAITDALLGAAALGDIGRHFPDTDAAFRAADSRLL
LAEAARRVRAAGYEIGNIDSTVVAQAPRLAAHIPAMRLAIARALGVDQGQVNVKAKTAEGLGPVGQNLAIEARAVALICA
TPGAGAIGPAAQRVSSAWSGSGA
>Mature_183_residues
MNLRIGEGWDVHALVPGRRLVIGGVELEHPMGLGLLGHSDADVLLHAITDALLGAAALGDIGRHFPDTDAAFRAADSRLL
LAEAARRVRAAGYEIGNIDSTVVAQAPRLAAHIPAMRLAIARALGVDQGQVNVKAKTAEGLGPVGQNLAIEARAVALICA
TPGAGAIGPAAQRVSSAWSGSGA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=57.6923076923077, Blast_Score=162, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_VEREI (A1WR07)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999082.1
- ProteinModelPortal:   A1WR07
- SMR:   A1WR07
- STRING:   A1WR07
- GeneID:   4694030
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4361
- NMPDR:   fig|391735.5.peg.3993
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A1WR07
- BioCyc:   VEIS391735:VEIS_4361-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 18606; Mature: 18606

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRIGEGWDVHALVPGRRLVIGGVELEHPMGLGLLGHSDADVLLHAITDALLGAAALGD
CCCCCCCCCCEEEECCCCEEEEECEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IGRHFPDTDAAFRAADSRLLLAEAARRVRAAGYEIGNIDSTVVAQAPRLAAHIPAMRLAI
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ARALGVDQGQVNVKAKTAEGLGPVGQNLAIEARAVALICATPGAGAIGPAAQRVSSAWSG
HHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCC
SGA
CCC
>Mature Secondary Structure
MNLRIGEGWDVHALVPGRRLVIGGVELEHPMGLGLLGHSDADVLLHAITDALLGAAALGD
CCCCCCCCCCEEEECCCCEEEEECEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IGRHFPDTDAAFRAADSRLLLAEAARRVRAAGYEIGNIDSTVVAQAPRLAAHIPAMRLAI
HHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ARALGVDQGQVNVKAKTAEGLGPVGQNLAIEARAVALICATPGAGAIGPAAQRVSSAWSG
HHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCC
SGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA