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The map label for this gene is acnB [H]

Identifier: 121611272

GI number: 121611272

Start: 4800792

End: 4803383

Strand: Direct

Name: acnB [H]

Synonym: Veis_4358

Alternate gene names: 121611272

Gene position: 4800792-4803383 (Clockwise)

Preceding gene: 121611271

Following gene: 121611274

Centisome position: 86.24

GC content: 66.63

Gene sequence:

>2592_bases
ATGTTGCAAGCCTACAGAGACCATGCCGCCGAGCGCGCTGCCCTGGGCATTCCCCCGCTGCCCCTGAATGCCGGGCAGGT
TGCCGCATTGGTCGATCTGATCCGGAATCCGCCGGCTGGCGAAGCCGCTTTCTTGCTCGATCTGCTGGCCCGGCGCGTGC
CGCCGGGGGTGGACGACGCGGCCAAGGTCAAGGCCAGTTTTCTGGCTGCGGTCGCGCATGGCGAGTTGGCGGTCGAGTCG
ATCTCCAAGGCCCGGGCGACCGAGTTGTTGGGCACCATGGTCGGCGGCTACAACGTGCAGCCGTTGATCGAGTTGCTCGA
TGACGCGCCAGTGGCTGACGTGGCCGCCGAGGGCCTGAAGAAAACCCTGCTGATGTTCGACTTCTTCAACGATGTCGCGG
CCAAGGCCCGGGCCGGCCACGCCAAGGCGCTGGAGGTGATGCACGGCTGGGCCGATGCCCAGTGGTTCACCTGCCGGCCC
GAGGTCGCCCAAAAGATCACCGTCACCGTGTTCAAGGTGCCCGGCGAGACCAATACCGATGACCTGTCGCCGGCGCCTGA
CGCCTGGAGCCGCCCGGACATTCCGCTGCACGCGCTGGCGATGCTCAAGAACCGCCGCGAGGGCGCGGCCTTCACGCCCG
AAAAAGACGGCCAGCGCGGCCCGATGCAGTTCATTGCAGACCTCAAGAGCCGGGGCCATCTGGTGGCCTATGTGGGCGAT
GTGGTGGGCACGGGGTCGAGCCGCAAGTCCGCCTCCAACAGTCTGATCTGGGCCACGGGCCAGGACATTCCGTTCGTGCC
GAACAAGCGCTTTGGCGGGGTGACTTTGGGCGGCAAGATCGCGCCGATCTTTTTCAACACCCAGGAAGATTCCGGCGCGC
TGCCGATCGAGGTCGATGTGTCGCGGCTGGAAATGGGCGATGTGCTCGACATCCTGCCCTACGAGGGACTGATCCTGCGC
GGTGGCCAGACCGTGGCCGAGTTCCGGCTCAAGAGCCAGGTGCTGTTGGACGAAGTACGCGCCGGCGGCCGCATCAACCT
GATCATTGGCCGCTCGCTGACCGCCAAGGCGCGCGAATTCCTGGGCCTGGCGGCCGCCACCTGCTTTCGTCTGCCGGCCG
CCCCGGTGGCCACCCAGGCCGGTTTCACGCTGGCGCAAAAGATGGTCGGCCGCGCCGTCGGCCTGCCCGAAGGCCAGGGC
GTGCGCCCCGGCACCTACTGCGAGCCCCGGATGACGACCGTAGGCTCGCAAGACACCACCGGCCCGATGACCCGCGATGA
GTTGAAAGACCTGGCCTGCCTGGGTTTTTCGGCCGATCTGGTGATGCAGTCGTTTTGCCACACCGCCGCTTACCCCAAGC
CGGTGGACGTGAAGACGCACCGCGAACTGCCGGCCTTCATCAGCCACCGTGGCGGCGTGGCGCTGCGCCCCGGCGACGGC
GTGATCCACAGTTGGCTCAACCGCTTGTTGCTGCCCGACACCGTGGGCACCGGCGCCGACTCGCACACGCGCTTTCCGAT
CGGCATTTCATTCCCCGCAGGCTCGGGCCTGGTGGCCTTCGGCGCGGCCACCGGCGTGATGCCGCTGGACATGCCCGAAT
CGGTGCTGGTGCGCTTCAAAGGCCGGATGCAGCCGGGCGTGACGCTGCGCGACCTGGTGCACGCGATTGCGCTGTACGCG
ATCCGCGCCGGTCTGCTGACCGTGGCCAAGGCCGGCAAGAAGAACATCTTCTCGGGCCGCATTCTGGAAATCGAGGGTCT
GCCGGACTTGAAGGTCGAGCAGGCTTTCGAGCTGTCGGATGCGTCGGCAGAGCGCTCTGCCGCAGGCTGCACGATTGCGC
TGAACCCGGCCCCGGTCCAAGAGTATCTGCGCAGCAACCTGGTGCTGATGAAGCGCATGATTGCCGACGGTTATGCCGAC
GCCCGCACGCTGCAACGGCGCATCGACAAGGTTCAGGCCTGGCTGGACCAGCCCAAGTTGCTCCAAGCCGACCGGGACGC
CGAATACGCGGCCGTGATCGACATCGATCTGGCCGACATCGAGGAGCCTGTCTTGTGCTGCCCGAACGACCCGGACGACG
CCAAGACCCTGTCCGAGGTGGCCGGCACGAAGATCGATGAGGCGTTCATCGGTTCCTGCATGACCAATATCGGCCATTTC
CGTGCCGCCGCGAAGCTGCTCGGCGGCAGCCGCGACATTCCCGTCAAGCTGTGGGTGGCGCCGCCGACCCGGATGGACCA
GAGCGAGTTGATCAAGGAAGGCCACTATGCGAGCTTCGGCGCCGCCGGCGCCCGCACCGAAATGCCCGGCTGCTCGCTGT
GCATGGGCAATCAGGCCCAGGTTCGGGAAGGCGCCACTGTGGTCTCGACCTCCACGCGCAACTTCCCGAACCGCCTGGGC
AAGAACACCCAGGTGTTCCTGGCCTCGGCGGAACTGGCTGCGGTGGCGTCCAAGCTGGGCCGGCTGCCGACGCTGGCCGA
GTACCAGCAGGCCATGGGCATCATCGCCCAGGATGCGGCCGACACCTACCGCTATCTGAACTTCGACCAGATCGAGGAAT
ACGCCAGCGCGGCCAACGGCGTTGCGGCCTAG

Upstream 100 bases:

>100_bases
ATACCCGACTGACGCCCAAGTGGCCCCCCAGTGACCCGGCAAGCCACGCGGCGCGCCCCCTCCCCCCGATTCGAATGACT
CCCCTCACTGGAGAAAAAAG

Downstream 100 bases:

>100_bases
GCCGCAACGCCGTTGGCCATATGGGCCACATGGGCCACCTCACGGCGGCGCGACCTCGGCGAGCGGCGCGCCGAACTGGC
GCAGCACCTCGCGCACCAGC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MLQAYRDHAAERAALGIPPLPLNAGQVAALVDLIRNPPAGEAAFLLDLLARRVPPGVDDAAKVKASFLAAVAHGELAVES
ISKARATELLGTMVGGYNVQPLIELLDDAPVADVAAEGLKKTLLMFDFFNDVAAKARAGHAKALEVMHGWADAQWFTCRP
EVAQKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHALAMLKNRREGAAFTPEKDGQRGPMQFIADLKSRGHLVAYVGD
VVGTGSSRKSASNSLIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIEVDVSRLEMGDVLDILPYEGLILR
GGQTVAEFRLKSQVLLDEVRAGGRINLIIGRSLTAKAREFLGLAAATCFRLPAAPVATQAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHRELPAFISHRGGVALRPGDG
VIHSWLNRLLLPDTVGTGADSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGRMQPGVTLRDLVHAIALYA
IRAGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIALNPAPVQEYLRSNLVLMKRMIADGYAD
ARTLQRRIDKVQAWLDQPKLLQADRDAEYAAVIDIDLADIEEPVLCCPNDPDDAKTLSEVAGTKIDEAFIGSCMTNIGHF
RAAAKLLGGSRDIPVKLWVAPPTRMDQSELIKEGHYASFGAAGARTEMPGCSLCMGNQAQVREGATVVSTSTRNFPNRLG
KNTQVFLASAELAAVASKLGRLPTLAEYQQAMGIIAQDAADTYRYLNFDQIEEYASAANGVAA

Sequences:

>Translated_863_residues
MLQAYRDHAAERAALGIPPLPLNAGQVAALVDLIRNPPAGEAAFLLDLLARRVPPGVDDAAKVKASFLAAVAHGELAVES
ISKARATELLGTMVGGYNVQPLIELLDDAPVADVAAEGLKKTLLMFDFFNDVAAKARAGHAKALEVMHGWADAQWFTCRP
EVAQKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHALAMLKNRREGAAFTPEKDGQRGPMQFIADLKSRGHLVAYVGD
VVGTGSSRKSASNSLIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIEVDVSRLEMGDVLDILPYEGLILR
GGQTVAEFRLKSQVLLDEVRAGGRINLIIGRSLTAKAREFLGLAAATCFRLPAAPVATQAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHRELPAFISHRGGVALRPGDG
VIHSWLNRLLLPDTVGTGADSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGRMQPGVTLRDLVHAIALYA
IRAGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIALNPAPVQEYLRSNLVLMKRMIADGYAD
ARTLQRRIDKVQAWLDQPKLLQADRDAEYAAVIDIDLADIEEPVLCCPNDPDDAKTLSEVAGTKIDEAFIGSCMTNIGHF
RAAAKLLGGSRDIPVKLWVAPPTRMDQSELIKEGHYASFGAAGARTEMPGCSLCMGNQAQVREGATVVSTSTRNFPNRLG
KNTQVFLASAELAAVASKLGRLPTLAEYQQAMGIIAQDAADTYRYLNFDQIEEYASAANGVAA
>Mature_863_residues
MLQAYRDHAAERAALGIPPLPLNAGQVAALVDLIRNPPAGEAAFLLDLLARRVPPGVDDAAKVKASFLAAVAHGELAVES
ISKARATELLGTMVGGYNVQPLIELLDDAPVADVAAEGLKKTLLMFDFFNDVAAKARAGHAKALEVMHGWADAQWFTCRP
EVAQKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHALAMLKNRREGAAFTPEKDGQRGPMQFIADLKSRGHLVAYVGD
VVGTGSSRKSASNSLIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIEVDVSRLEMGDVLDILPYEGLILR
GGQTVAEFRLKSQVLLDEVRAGGRINLIIGRSLTAKAREFLGLAAATCFRLPAAPVATQAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHRELPAFISHRGGVALRPGDG
VIHSWLNRLLLPDTVGTGADSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGRMQPGVTLRDLVHAIALYA
IRAGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIALNPAPVQEYLRSNLVLMKRMIADGYAD
ARTLQRRIDKVQAWLDQPKLLQADRDAEYAAVIDIDLADIEEPVLCCPNDPDDAKTLSEVAGTKIDEAFIGSCMTNIGHF
RAAAKLLGGSRDIPVKLWVAPPTRMDQSELIKEGHYASFGAAGARTEMPGCSLCMGNQAQVREGATVVSTSTRNFPNRLG
KNTQVFLASAELAAVASKLGRLPTLAEYQQAMGIIAQDAADTYRYLNFDQIEEYASAANGVAA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=864, Percent_Identity=70.7175925925926, Blast_Score=1206, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=377, Percent_Identity=25.9946949602122, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=495, Percent_Identity=23.2323232323232, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92139; Mature: 92139

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQAYRDHAAERAALGIPPLPLNAGQVAALVDLIRNPPAGEAAFLLDLLARRVPPGVDDA
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKASFLAAVAHGELAVESISKARATELLGTMVGGYNVQPLIELLDDAPVADVAAEGLK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDFFNDVAAKARAGHAKALEVMHGWADAQWFTCRPEVAQKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECHHHHCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNRREGAAFTPEKDGQRGPMQFIADLKSRGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECH
VVGTGSSRKSASNSLIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEEEH
SRLEMGDVLDILPYEGLILRGGQTVAEFRLKSQVLLDEVRAGGRINLIIGRSLTAKAREF
HHHCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LGLAAATCFRLPAAPVATQAGFTLAQKMVGRAVGLPEGQGVRPGTYCEPRMTTVGSQDTT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHRELPAFISHRGGVALRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCC
VIHSWLNRLLLPDTVGTGADSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHH
GRMQPGVTLRDLVHAIALYAIRAGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTIALNPAPVQEYLRSNLVLMKRMIADGYADARTLQRRIDKVQAWLDQPKL
CCCHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
LQADRDAEYAAVIDIDLADIEEPVLCCPNDPDDAKTLSEVAGTKIDEAFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAAAKLLGGSRDIPVKLWVAPPTRMDQSELIKEGHYASFGAAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCHHH
VREGATVVSTSTRNFPNRLGKNTQVFLASAELAAVASKLGRLPTLAEYQQAMGIIAQDAA
HHCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DTYRYLNFDQIEEYASAANGVAA
HHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLQAYRDHAAERAALGIPPLPLNAGQVAALVDLIRNPPAGEAAFLLDLLARRVPPGVDDA
CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKASFLAAVAHGELAVESISKARATELLGTMVGGYNVQPLIELLDDAPVADVAAEGLK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDFFNDVAAKARAGHAKALEVMHGWADAQWFTCRPEVAQKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEECHHHHCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNRREGAAFTPEKDGQRGPMQFIADLKSRGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECH
VVGTGSSRKSASNSLIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGALPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEEEH
SRLEMGDVLDILPYEGLILRGGQTVAEFRLKSQVLLDEVRAGGRINLIIGRSLTAKAREF
HHHCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LGLAAATCFRLPAAPVATQAGFTLAQKMVGRAVGLPEGQGVRPGTYCEPRMTTVGSQDTT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHRELPAFISHRGGVALRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCC
VIHSWLNRLLLPDTVGTGADSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHH
GRMQPGVTLRDLVHAIALYAIRAGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTIALNPAPVQEYLRSNLVLMKRMIADGYADARTLQRRIDKVQAWLDQPKL
CCCHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
LQADRDAEYAAVIDIDLADIEEPVLCCPNDPDDAKTLSEVAGTKIDEAFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAAAKLLGGSRDIPVKLWVAPPTRMDQSELIKEGHYASFGAAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCHHH
VREGATVVSTSTRNFPNRLGKNTQVFLASAELAAVASKLGRLPTLAEYQQAMGIIAQDAA
HHCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DTYRYLNFDQIEEYASAANGVAA
HHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]