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The map label for this gene is miaA

Identifier: 121611143

GI number: 121611143

Start: 4658912

End: 4659937

Strand: Direct

Name: miaA

Synonym: Veis_4227

Alternate gene names: 121611143

Gene position: 4658912-4659937 (Clockwise)

Preceding gene: 121611142

Following gene: 121611150

Centisome position: 83.69

GC content: 72.32

Gene sequence:

>1026_bases
GTGGCCGCGCCTGACAGCCGGCTCCCGAACATCGCCCTGGCAGGCCCCACGGCGGCGGGCAAGACGGCGGCGGCGCTGGC
GCTGGCGGCGGCGCTGGGCCGGCGCCAAGCGGTGGAGATCATCAGCGTGGACTCGGCCCTGGTCTACCGGGGCATGGACA
TCGGCAGCGCCAAGCCCACGCCGGCCGAGCGCGCCGCCGTGCCGCACCACCTGATCGACATCCGCGACCCGCTACAGGCT
TACAGCGCCGCCGAGTTCGTGCAAGACGCCCGCCGGCTCATCGGCGAAATCCGTGCCCGTGGCGCGTTGCCGTTGCTGGT
GGGCGGCACCATGCTGTACTTCAAGGCTTTGTTCGACGGCCTGGACGCCATGCCCGCCGCCGACCCCGCCGTGCGCGCGC
GGCTGAATGCCCGGGCCGCCGAGCAGGGCTGGCCCGCGCTGCACACCGAACTGGCCCGCGTCGACCCCGTGACCGCCGCC
CGACTGGCCCCCGGCGACAGCCAGCGCATACAGCGCGCTTTGGAGGTGTGGCAGATATCGGGCCAGCCGCTGTCGAGCTT
TCATGCCATCGAAAAAGGAGCTGCCGGCGCATATGGTGCTTGCGCCTGCGCATTGTTTTCGTTGGAACCGCAAGACCGTG
CCTGGCTGCACGAGCGCATCGCCCGACGCTTTGACGCCATGCTCGCCGCCGGCTTCATCGCCGAAGTGCAGGCCCTGCGC
GCGCGCGGCGACCTGCACCCCGACCTGCCCGCGATGCGCTGCGTGGGCTATCGCCAGGTCTGGGAGGCGCTCGATTGGCA
AGCCCGCCACGCCGGCGGCCCGCCGTTGCATGGGGCCCCGCTGCATGCGCGCGGCATGGACGCGGTGCGCGAGCGCGGCG
TGGCCGCCACACGCCAACTGGCCAAGCGCCAAATCACCTGGCTGCGCAGCATGCCGCAGCGCCACACCACGGCCTGCGAC
CAGCCCCAGGCCGTGGCCCAACTGGTGCAGGCGGTGTTGCAGCGGCTGGAGCAGCACGCTCCCTGA

Upstream 100 bases:

>100_bases
GGCATCTGGTTCTGGTCGGTATTGCTGGCACTGGTCGCGTGGACACTGGTGCAAAAGTTCGGTGACAGCACGGCCGCAGG
CACCTCGGCCGGAGACCGCA

Downstream 100 bases:

>100_bases
GTGCCACTGAATGGCGCTGAATGGCACCGGCGCGCTCAGAGCAGCGCCAGCCGTTCCCACCCCGGCGCCGCAGCACCCGT
CAGCGCCAGCCGCAGCACCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAAPDSRLPNIALAGPTAAGKTAAALALAAALGRRQAVEIISVDSALVYRGMDIGSAKPTPAERAAVPHHLIDIRDPLQA
YSAAEFVQDARRLIGEIRARGALPLLVGGTMLYFKALFDGLDAMPAADPAVRARLNARAAEQGWPALHTELARVDPVTAA
RLAPGDSQRIQRALEVWQISGQPLSSFHAIEKGAAGAYGACACALFSLEPQDRAWLHERIARRFDAMLAAGFIAEVQALR
ARGDLHPDLPAMRCVGYRQVWEALDWQARHAGGPPLHGAPLHARGMDAVRERGVAATRQLAKRQITWLRSMPQRHTTACD
QPQAVAQLVQAVLQRLEQHAP

Sequences:

>Translated_341_residues
MAAPDSRLPNIALAGPTAAGKTAAALALAAALGRRQAVEIISVDSALVYRGMDIGSAKPTPAERAAVPHHLIDIRDPLQA
YSAAEFVQDARRLIGEIRARGALPLLVGGTMLYFKALFDGLDAMPAADPAVRARLNARAAEQGWPALHTELARVDPVTAA
RLAPGDSQRIQRALEVWQISGQPLSSFHAIEKGAAGAYGACACALFSLEPQDRAWLHERIARRFDAMLAAGFIAEVQALR
ARGDLHPDLPAMRCVGYRQVWEALDWQARHAGGPPLHGAPLHARGMDAVRERGVAATRQLAKRQITWLRSMPQRHTTACD
QPQAVAQLVQAVLQRLEQHAP
>Mature_340_residues
AAPDSRLPNIALAGPTAAGKTAAALALAAALGRRQAVEIISVDSALVYRGMDIGSAKPTPAERAAVPHHLIDIRDPLQAY
SAAEFVQDARRLIGEIRARGALPLLVGGTMLYFKALFDGLDAMPAADPAVRARLNARAAEQGWPALHTELARVDPVTAAR
LAPGDSQRIQRALEVWQISGQPLSSFHAIEKGAAGAYGACACALFSLEPQDRAWLHERIARRFDAMLAAGFIAEVQALRA
RGDLHPDLPAMRCVGYRQVWEALDWQARHAGGPPLHGAPLHARGMDAVRERGVAATRQLAKRQITWLRSMPQRHTTACDQ
PQAVAQLVQAVLQRLEQHAP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=322, Percent_Identity=29.1925465838509, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1790613, Length=323, Percent_Identity=52.6315789473684, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25144712, Length=320, Percent_Identity=26.25, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=305, Percent_Identity=27.8688524590164, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24649830, Length=344, Percent_Identity=28.4883720930233, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_VEREI (A1WQM5)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_998950.1
- ProteinModelPortal:   A1WQM5
- SMR:   A1WQM5
- STRING:   A1WQM5
- GeneID:   4694454
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4227
- NMPDR:   fig|391735.5.peg.3876
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- PhylomeDB:   A1WQM5
- ProtClustDB:   CLSK963016
- BioCyc:   VEIS391735:VEIS_4227-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36692; Mature: 36560

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPDSRLPNIALAGPTAAGKTAAALALAAALGRRQAVEIISVDSALVYRGMDIGSAKPT
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
PAERAAVPHHLIDIRDPLQAYSAAEFVQDARRLIGEIRARGALPLLVGGTMLYFKALFDG
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDAMPAADPAVRARLNARAAEQGWPALHTELARVDPVTAARLAPGDSQRIQRALEVWQIS
HCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCC
GQPLSSFHAIEKGAAGAYGACACALFSLEPQDRAWLHERIARRFDAMLAAGFIAEVQALR
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGDLHPDLPAMRCVGYRQVWEALDWQARHAGGPPLHGAPLHARGMDAVRERGVAATRQL
HHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHH
AKRQITWLRSMPQRHTTACDQPQAVAQLVQAVLQRLEQHAP
HHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAPDSRLPNIALAGPTAAGKTAAALALAAALGRRQAVEIISVDSALVYRGMDIGSAKPT
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
PAERAAVPHHLIDIRDPLQAYSAAEFVQDARRLIGEIRARGALPLLVGGTMLYFKALFDG
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDAMPAADPAVRARLNARAAEQGWPALHTELARVDPVTAARLAPGDSQRIQRALEVWQIS
HCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCC
GQPLSSFHAIEKGAAGAYGACACALFSLEPQDRAWLHERIARRFDAMLAAGFIAEVQALR
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGDLHPDLPAMRCVGYRQVWEALDWQARHAGGPPLHGAPLHARGMDAVRERGVAATRQL
HHCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHH
AKRQITWLRSMPQRHTTACDQPQAVAQLVQAVLQRLEQHAP
HHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA