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The map label for this gene is livM [H]

Identifier: 121611012

GI number: 121611012

Start: 4493133

End: 4494209

Strand: Direct

Name: livM [H]

Synonym: Veis_4096

Alternate gene names: 121611012

Gene position: 4493133-4494209 (Clockwise)

Preceding gene: 121611011

Following gene: 121611013

Centisome position: 80.71

GC content: 66.3

Gene sequence:

>1077_bases
ATGCGCTTTCTGTTCAAGACCTCCTACGCGCAGGATCTGCGGCTGGCCCGGCATGGCGGGCATGTCTTTTGGTACGGGCT
GCTGTGCCTGGCGCTGGTGCTGGCGCCCTGGGTGGTGCAGGAATACTGGCTGGCGCAGCTCAGCTTCGTGCTGATCTATG
CGATCGTGGGCCTGGGGCTGATGCTGCTGGCCGGCTTTACCGGCCTGTTTTCGCTCGGTCATGCGGCCTTTCTGGGCGTG
GGGGCGTACACGCAGGCCGGGCTGACCGGCATCGGCTGGCCGTTTGCGCTGGCGCTGGTCTGCGCCGCCGCGCTGTCCGC
TGCGGCGGGCTGGGTGGTGGGCTTGCCCGCGCTGCGCGTCAAGGGCATCTACCTGGGCATGGCCACGCTGTCGTTCGGGT
TCATCGTCGAAGAGCTGTTTGCCCGCTGGGAGTCGGTCACCGGCGGCAATGCCGGCAAGCACCTGGCGCCGCCGCAGGTC
TGGGGCCTGAGCCTGGAGTCCGGCACCGCTTTCCATTTTCTGTGCCTGGGTCTGACCGTGGTCTGTACGCTGGCCGTCCT
GAACCTGCTGCGCTCGCCCACGGGCCGGGCCTTCGTCGCGATCCGCGATTCGGAGATCTCGGCGCAGAGCATGGGCATTC
ACCTGGCGCGCTACAAGACGCTGTCGTTTTCGCTGTCGGCGGCGCTGGCGGGCATCGGCGGCGCGCTGTATGCGCACAAG
CTGCAATTCATCTCGCCGGACCAATTCGGCATCATGCAGTCGATCGACCTGCTGCTGATGGTCGTGGTCGGCGGGCTGGG
CTCGGTGCACGGCGCTTTTCTGGGGGCCCTGTTCCTGATTGCGATGCCGCAACTGATCGCGCTGGGCAAGGACTGGCTGC
CCGCCACCATCGGCCAGGCGCCCGGGTTGCAGGCCGTGGTCTATGGCGCGGTGCTGATCGCTTTCGTGCTGTTCGAGCCT
ATGGGTCTGTATGGCCGCTGGCTCAAGCTGCGTACCTGGCTGCAGATGTTTCCGTTCTACCGCCGGGGCATGTTCAAGCG
GCAGCGGTCGTTCCAGAAATCCGAGCGTTTGCGATGA

Upstream 100 bases:

>100_bases
AGGGCTTCAAGGACATTGCCGCTTTCATCGTGGTGCTCGTCATGCTGATGCTGCGCCCCAATGGTTTGTTTGGCGAGCCG
TTGCGCAAGAAAGTCTGACG

Downstream 100 bases:

>100_bases
CCACGCCGGCAGCCCCCCAGGACGACAGCGGCGTGCTGCTCAGCGTGCGCAACCTCAGCCTGCGCTTTGGCGGTGTGCTG
GCGGTCAACGACGTGAGCTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRFLFKTSYAQDLRLARHGGHVFWYGLLCLALVLAPWVVQEYWLAQLSFVLIYAIVGLGLMLLAGFTGLFSLGHAAFLGV
GAYTQAGLTGIGWPFALALVCAAALSAAAGWVVGLPALRVKGIYLGMATLSFGFIVEELFARWESVTGGNAGKHLAPPQV
WGLSLESGTAFHFLCLGLTVVCTLAVLNLLRSPTGRAFVAIRDSEISAQSMGIHLARYKTLSFSLSAALAGIGGALYAHK
LQFISPDQFGIMQSIDLLLMVVVGGLGSVHGAFLGALFLIAMPQLIALGKDWLPATIGQAPGLQAVVYGAVLIAFVLFEP
MGLYGRWLKLRTWLQMFPFYRRGMFKRQRSFQKSERLR

Sequences:

>Translated_358_residues
MRFLFKTSYAQDLRLARHGGHVFWYGLLCLALVLAPWVVQEYWLAQLSFVLIYAIVGLGLMLLAGFTGLFSLGHAAFLGV
GAYTQAGLTGIGWPFALALVCAAALSAAAGWVVGLPALRVKGIYLGMATLSFGFIVEELFARWESVTGGNAGKHLAPPQV
WGLSLESGTAFHFLCLGLTVVCTLAVLNLLRSPTGRAFVAIRDSEISAQSMGIHLARYKTLSFSLSAALAGIGGALYAHK
LQFISPDQFGIMQSIDLLLMVVVGGLGSVHGAFLGALFLIAMPQLIALGKDWLPATIGQAPGLQAVVYGAVLIAFVLFEP
MGLYGRWLKLRTWLQMFPFYRRGMFKRQRSFQKSERLR
>Mature_358_residues
MRFLFKTSYAQDLRLARHGGHVFWYGLLCLALVLAPWVVQEYWLAQLSFVLIYAIVGLGLMLLAGFTGLFSLGHAAFLGV
GAYTQAGLTGIGWPFALALVCAAALSAAAGWVVGLPALRVKGIYLGMATLSFGFIVEELFARWESVTGGNAGKHLAPPQV
WGLSLESGTAFHFLCLGLTVVCTLAVLNLLRSPTGRAFVAIRDSEISAQSMGIHLARYKTLSFSLSAALAGIGGALYAHK
LQFISPDQFGIMQSIDLLLMVVVGGLGSVHGAFLGALFLIAMPQLIALGKDWLPATIGQAPGLQAVVYGAVLIAFVLFEP
MGLYGRWLKLRTWLQMFPFYRRGMFKRQRSFQKSERLR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=278, Percent_Identity=34.8920863309352, Blast_Score=130, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38780; Mature: 38780

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLFKTSYAQDLRLARHGGHVFWYGLLCLALVLAPWVVQEYWLAQLSFVLIYAIVGLGL
CCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLAGFTGLFSLGHAAFLGVGAYTQAGLTGIGWPFALALVCAAALSAAAGWVVGLPALRV
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KGIYLGMATLSFGFIVEELFARWESVTGGNAGKHLAPPQVWGLSLESGTAFHFLCLGLTV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHH
VCTLAVLNLLRSPTGRAFVAIRDSEISAQSMGIHLARYKTLSFSLSAALAGIGGALYAHK
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQFISPDQFGIMQSIDLLLMVVVGGLGSVHGAFLGALFLIAMPQLIALGKDWLPATIGQA
HHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
PGLQAVVYGAVLIAFVLFEPMGLYGRWLKLRTWLQMFPFYRRGMFKRQRSFQKSERLR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFLFKTSYAQDLRLARHGGHVFWYGLLCLALVLAPWVVQEYWLAQLSFVLIYAIVGLGL
CCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLAGFTGLFSLGHAAFLGVGAYTQAGLTGIGWPFALALVCAAALSAAAGWVVGLPALRV
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KGIYLGMATLSFGFIVEELFARWESVTGGNAGKHLAPPQVWGLSLESGTAFHFLCLGLTV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHH
VCTLAVLNLLRSPTGRAFVAIRDSEISAQSMGIHLARYKTLSFSLSAALAGIGGALYAHK
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQFISPDQFGIMQSIDLLLMVVVGGLGSVHGAFLGALFLIAMPQLIALGKDWLPATIGQA
HHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
PGLQAVVYGAVLIAFVLFEPMGLYGRWLKLRTWLQMFPFYRRGMFKRQRSFQKSERLR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]