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The map label for this gene is pilB [H]

Identifier: 121610837

GI number: 121610837

Start: 4306572

End: 4308239

Strand: Direct

Name: pilB [H]

Synonym: Veis_3919

Alternate gene names: 121610837

Gene position: 4306572-4308239 (Clockwise)

Preceding gene: 121610836

Following gene: 121610838

Centisome position: 77.36

GC content: 57.91

Gene sequence:

>1668_bases
ATGTCCGCAGGCAAGCTGAGTCAGAAATCGGCCGAGGACATTTACAAGAAGTCCCAGGCCAACCGCACCAGTTTCATTGC
CGAATTGACCGGCTCCGGGGCTGTCTCCGCCGCTGACTTGGCGCATACGGTGTCGACCGTCTTTGGCGCGCCCCTTCTCG
ATCTCGATGCCATCGATCCGTCGCGTTTGCCAAAGGATTTGCTGGATAACAAGATATGCAAGACCTGCCGCGTCGTCGTA
CTGAGCAAACGCAGTAATCGCCTGATCGTCGCAACGGCAGACCCTACCGACCAGGAAGCGGCTGAAAAAATCAAGTTTGC
CACCCAGATGGGCGTGGACTGGGTCATCGCGGAATATGACAAACTCAGCCTTCTGGTCGAAGCGATCACCAAATCCGCCA
ACGAAGCAATGGATACGCTGGTCAGTGGCGGTGACTTCGAGTTCGACGATATGGCGGTCGAAACTGCCACCGAAGATGCG
GCTGTGGCAGCCACGGATGTCGAAGATGCTCCGATCGTCAAGTTTCTGCACAAAATGCTGCTGGACGCCTTCAACATGCG
CGCGTCCGACCTGCATTTCGAACCCTACGAGCACCAGTACCGCGTCCGCTTCCGCATCGATGGCGAGTTGCGGGAAATAG
CTTCGCCGCCGATTGCGATCAAGGAAAAGCTGGCTTCCCGCATCAAGGTCATTTCGCGCCTGGATATCTCGGAAAAACGC
ATTCCACAAGATGGCCGCATGAAGCTGAAAGTCGGCCAGAACCGGGTGATCGACTTCCGGGTCAGCACCTTGCCCACCTT
GTTTGGCGAGAAAGTCGTCATCCGTATTCTGGATCCGAGCAGCGCCAAACTGGGTATCGATGCGCTGGGCTATGAACCGG
TGGAGAAAGAGCGGCTACTCCAGGCCATCGGTCGCCCCTACGGCATGATTTTGGTCACAGGCCCTACCGGTTCGGGCAAG
ACCGTGTCACTGTATACCTGTCTGAATCTCCTGAACAAACCGGACGTCAATATCGCGACGGCAGAAGACCCTTGCGAAAT
CAACCTGCCGGGCGTGAATCAGGTAAACGTCAACGAAAAGGCCGGCCTGACATTTGCTGCGGCGCTGCGCTCCTTCCTGC
GCCAGGATCCCGACATCATCATGGTCGGGGAAATACGGGATCTGGAAACTGCCGACATCTCGATCAAGGCGGCACAAACC
GGGCACCTGGTGCTGTCCACCCTGCACACGAATGACGCGCCGACGACCTTGACACGGATGCGCAACATGGGTATTGCGCC
ATTCAATATTGCGTCCAGCGTGATTCTGATCACCGCGCAGCGCCTGGCGCGCAGGCTGTGCCCGATCTGCAAAACCCCGA
CGGACATACCCCAGGAGACCCTGGTCGATGCGGGATACCAAGAGGAGGAGATCGACGGCTCCTGGGTTACTTATGGTCCG
GTCGGCTGCAACGCTTGCAACAATGGCTACAAGGGCCGGGTCGGCATTTATCAGGTGATGCCGATCACAGAGGAAATCCA
GCGCATCATCCTGCGCGATGGCAGCGCCCTGGAGATTGCCGAGCAAGCCCGCATGGAGGGTGTGCGCTCATTGCGCGACG
CGGGCCTGTACAAGGCCAAGTTGGGCCTGACTTCCCTCGAAGAAGTCCTGGCCGTCACCAACGAATAA

Upstream 100 bases:

>100_bases
ATGCCTTTTTCCCCCTCCGCCGAACGGTGTCATGGCCGCTGTCGATACTCCCTCCAAAGAAGCCTCTGCCGTTGCACTTC
CCGGACTGGGCAGGGCGTTG

Downstream 100 bases:

>100_bases
CACATGGCATTTACCGGAGGGCAGCATGGCAACCATCACGGCAGCAAAAAGAGAGATCAAGGAAAAAGTCTTCGAATGGG
AAGGCAAGGACCGCAATGGA

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDAIDPSRLPKDLLDNKICKTCRVVV
LSKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYDKLSLLVEAITKSANEAMDTLVSGGDFEFDDMAVETATEDA
AVAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIAIKEKLASRIKVISRLDISEKR
IPQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKERLLQAIGRPYGMILVTGPTGSGK
TVSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEKAGLTFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQT
GHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIPQETLVDAGYQEEEIDGSWVTYGP
VGCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLYKAKLGLTSLEEVLAVTNE

Sequences:

>Translated_555_residues
MSAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDAIDPSRLPKDLLDNKICKTCRVVV
LSKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYDKLSLLVEAITKSANEAMDTLVSGGDFEFDDMAVETATEDA
AVAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIAIKEKLASRIKVISRLDISEKR
IPQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKERLLQAIGRPYGMILVTGPTGSGK
TVSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEKAGLTFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQT
GHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIPQETLVDAGYQEEEIDGSWVTYGP
VGCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLYKAKLGLTSLEEVLAVTNE
>Mature_554_residues
SAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDAIDPSRLPKDLLDNKICKTCRVVVL
SKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYDKLSLLVEAITKSANEAMDTLVSGGDFEFDDMAVETATEDAA
VAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIAIKEKLASRIKVISRLDISEKRI
PQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKERLLQAIGRPYGMILVTGPTGSGKT
VSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEKAGLTFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQTG
HLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIPQETLVDAGYQEEEIDGSWVTYGPV
GCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLYKAKLGLTSLEEVLAVTNE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=392, Percent_Identity=46.9387755102041, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1786296, Length=406, Percent_Identity=42.3645320197044, Blast_Score=331, Evalue=9e-92,
Organism=Escherichia coli, GI87082188, Length=237, Percent_Identity=32.9113924050633, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 60660; Mature: 60529

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDAIDP
CCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCH
SRLPKDLLDNKICKTCRVVVLSKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYD
HHCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECHH
KLSLLVEAITKSANEAMDTLVSGGDFEFDDMAVETATEDAAVAATDVEDAPIVKFLHKML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHH
LDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIAIKEKLASRIKVISRLDISEKR
HHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
IPQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKERLL
CCCCCCEEEEECCCEEEEEEHHHHHHHCCCEEEEEEECCCCCCCCCHHCCCCCCCHHHHH
QAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEK
HHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCEEECCCCCEECCCCC
AGLTFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRM
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHH
RNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIPQETLVDAGYQEEEIDGSWVTYGP
HHCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCCHHCCCCCEEEECC
VGCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLYKAK
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
LGLTSLEEVLAVTNE
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
SAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDAIDP
CCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCH
SRLPKDLLDNKICKTCRVVVLSKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYD
HHCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECHH
KLSLLVEAITKSANEAMDTLVSGGDFEFDDMAVETATEDAAVAATDVEDAPIVKFLHKML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHH
LDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIAIKEKLASRIKVISRLDISEKR
HHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
IPQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKERLL
CCCCCCEEEEECCCEEEEEEHHHHHHHCCCEEEEEEECCCCCCCCCHHCCCCCCCHHHHH
QAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEK
HHHCCCEEEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCEEECCCCCEECCCCC
AGLTFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRM
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHH
RNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIPQETLVDAGYQEEEIDGSWVTYGP
HHCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCCHHCCCCCEEEECC
VGCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLYKAK
CCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
LGLTSLEEVLAVTNE
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]