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The map label for this gene is ispB [H]

Identifier: 121610836

GI number: 121610836

Start: 4305211

End: 4306239

Strand: Direct

Name: ispB [H]

Synonym: Veis_3918

Alternate gene names: 121610836

Gene position: 4305211-4306239 (Clockwise)

Preceding gene: 121610821

Following gene: 121610837

Centisome position: 77.34

GC content: 66.18

Gene sequence:

>1029_bases
ATGATCCGTGCACCTTCCGTGACCACCCTCTCGACCGCCCCCCCCATCGCCCCCGCTGCCCTCACCGCAGCATCCCCGTT
GGCACTGGTGGCCGACGACATGCGCGAGGTCGACAAAGTCATCGGCCAGCGCCTGCTATCGAACGTCCCGCTGGTGGATT
GGGTGGCGCGACACATCATCGCCGCCGGAGGCAAGCGCTTGCGCCCTGCGCTGCTGCTGATGGTGTGCGGGGCGCTGGGC
TATGGCGGCGCGCACCGCTTGAGCCTGGCGGCCGTGGTCGAACTGATCCACACTGCCACATTGCTGCACGACGATGTCGT
CGACGCCTCCACCTTGCGCCGTGGTCGCCCCACAGCCAACGAAATCTTCGGTGCTCCGGCCTGTGTGCTGGTGGGCGACT
TCTTGCACACCCGCTCGTTCCAGACCATGGTGGAAACGGGGAACATGCGCGTCATGGAAATCCTCGCCGAAGCCACGAAC
GTGATCGCCGAGGGCGAGGTGCTGCAACTGATGAACATGCATGACGCAACGCTCGATGAAGCCGGCTATCTGCGTGTGAT
CCGTTCCAAAACCGCCAAGCTGTTCGAGGCCAGCGCCCGGCTCGGCGCCGTGCTGGCCCGCAGCAGCCCCGACATCGAGC
AAGCCTGCGCCACCTACGGGCAGGCCCTGGGCACCGCCTTTCAGATCATCGACGATGTCCTGGACTACGACGGCGACAGC
GCAGAGATGGGCAAGAACCTGGGCGACGACCTGCGCGAAGGCAAGGCCACGCTGCCACTGATCGCCGCGATGCAACGAGG
CAGCCCCGGGCAGGCGCTGATCGTGCGCCAAGCCATCAAGGAAGGCTCGACGGAGCAACTGGCCGAGATCGTCGGCATCG
TGCGCGCGACCGGCGCGCTCGAAGTCACCCGCGCCGCCGCCTGCACCGAAGCCCGCCGGGCAATGTCCGCTGCGCAGCAA
CTGCCATCGAATGCCTATTCGAAGGGTTTGCTAGAATTGGCGGCGCAGTTGCTGGAGCGACGCCACTGA

Upstream 100 bases:

>100_bases
CATGTTGACCCCTTCGAGTAAATTTCTGCGAAACGGATTTCCGCAGAACCGGCAAGTCTAACGCATCGCGCAACCGCAGC
CGTAGCCGCACCCTCTTCCC

Downstream 100 bases:

>100_bases
GCCTGGGGTATGGCATCGATGCCGGCCGGGGGGCATGGCGCGCAGGTGCCACAGCGATTTGCGGGGTGTAGCTTAGCCTG
GTAGAGCGCTACGTTCGGGA

Product: dimethylallyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIRAPSVTTLSTAPPIAPAALTAASPLALVADDMREVDKVIGQRLLSNVPLVDWVARHIIAAGGKRLRPALLLMVCGALG
YGGAHRLSLAAVVELIHTATLLHDDVVDASTLRRGRPTANEIFGAPACVLVGDFLHTRSFQTMVETGNMRVMEILAEATN
VIAEGEVLQLMNMHDATLDEAGYLRVIRSKTAKLFEASARLGAVLARSSPDIEQACATYGQALGTAFQIIDDVLDYDGDS
AEMGKNLGDDLREGKATLPLIAAMQRGSPGQALIVRQAIKEGSTEQLAEIVGIVRATGALEVTRAAACTEARRAMSAAQQ
LPSNAYSKGLLELAAQLLERRH

Sequences:

>Translated_342_residues
MIRAPSVTTLSTAPPIAPAALTAASPLALVADDMREVDKVIGQRLLSNVPLVDWVARHIIAAGGKRLRPALLLMVCGALG
YGGAHRLSLAAVVELIHTATLLHDDVVDASTLRRGRPTANEIFGAPACVLVGDFLHTRSFQTMVETGNMRVMEILAEATN
VIAEGEVLQLMNMHDATLDEAGYLRVIRSKTAKLFEASARLGAVLARSSPDIEQACATYGQALGTAFQIIDDVLDYDGDS
AEMGKNLGDDLREGKATLPLIAAMQRGSPGQALIVRQAIKEGSTEQLAEIVGIVRATGALEVTRAAACTEARRAMSAAQQ
LPSNAYSKGLLELAAQLLERRH
>Mature_342_residues
MIRAPSVTTLSTAPPIAPAALTAASPLALVADDMREVDKVIGQRLLSNVPLVDWVARHIIAAGGKRLRPALLLMVCGALG
YGGAHRLSLAAVVELIHTATLLHDDVVDASTLRRGRPTANEIFGAPACVLVGDFLHTRSFQTMVETGNMRVMEILAEATN
VIAEGEVLQLMNMHDATLDEAGYLRVIRSKTAKLFEASARLGAVLARSSPDIEQACATYGQALGTAFQIIDDVLDYDGDS
AEMGKNLGDDLREGKATLPLIAAMQRGSPGQALIVRQAIKEGSTEQLAEIVGIVRATGALEVTRAAACTEARRAMSAAQQ
LPSNAYSKGLLELAAQLLERRH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=351, Percent_Identity=29.9145299145299, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4758430, Length=251, Percent_Identity=24.7011952191235, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI83700220, Length=251, Percent_Identity=24.7011952191235, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1789578, Length=313, Percent_Identity=49.2012779552716, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786623, Length=262, Percent_Identity=31.6793893129771, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=318, Percent_Identity=29.874213836478, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=30.5647840531561, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=261, Percent_Identity=26.4367816091954, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651612, Length=343, Percent_Identity=27.4052478134111, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=277, Percent_Identity=24.5487364620939, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24660002, Length=277, Percent_Identity=24.5487364620939, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36260; Mature: 36260

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAPSVTTLSTAPPIAPAALTAASPLALVADDMREVDKVIGQRLLSNVPLVDWVARHII
CCCCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAGGKRLRPALLLMVCGALGYGGAHRLSLAAVVELIHTATLLHDDVVDASTLRRGRPTAN
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHH
EIFGAPACVLVGDFLHTRSFQTMVETGNMRVMEILAEATNVIAEGEVLQLMNMHDATLDE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
AGYLRVIRSKTAKLFEASARLGAVLARSSPDIEQACATYGQALGTAFQIIDDVLDYDGDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AEMGKNLGDDLREGKATLPLIAAMQRGSPGQALIVRQAIKEGSTEQLAEIVGIVRATGAL
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHH
EVTRAAACTEARRAMSAAQQLPSNAYSKGLLELAAQLLERRH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRAPSVTTLSTAPPIAPAALTAASPLALVADDMREVDKVIGQRLLSNVPLVDWVARHII
CCCCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAGGKRLRPALLLMVCGALGYGGAHRLSLAAVVELIHTATLLHDDVVDASTLRRGRPTAN
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHH
EIFGAPACVLVGDFLHTRSFQTMVETGNMRVMEILAEATNVIAEGEVLQLMNMHDATLDE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
AGYLRVIRSKTAKLFEASARLGAVLARSSPDIEQACATYGQALGTAFQIIDDVLDYDGDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AEMGKNLGDDLREGKATLPLIAAMQRGSPGQALIVRQAIKEGSTEQLAEIVGIVRATGAL
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHH
EVTRAAACTEARRAMSAAQQLPSNAYSKGLLELAAQLLERRH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]