Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 121610821

GI number: 121610821

Start: 4292313

End: 4292786

Strand: Direct

Name: smpB

Synonym: Veis_3903

Alternate gene names: 121610821

Gene position: 4292313-4292786 (Clockwise)

Preceding gene: 121610818

Following gene: 121610836

Centisome position: 77.11

GC content: 61.18

Gene sequence:

>474_bases
ATGACCAAAAAACCCGATCCTGCGTCCCGCATTGCCGACAACAAGAAAGCGGCCCACGACTATTTCCTGGAAGAACGCTA
CGAAGCAGGCATGGTGCTGCATGGCTGGGAGGTCAAGGCGTTGCGCGCGGGCAAGGCACAGCTGACCGACGGCTATGTGC
TGATTCGCGACGGCGAGCTGTTTTTGCTCGGCTGCCAGCTCCAAGCGCTCAAGACCGCGTCCACCCATGTCAGCCCCGAG
GCGGCGCGCACGAAGAAATTGCTGCTCAAGAAGGACGACATCCGGCGCCTGATCGGCAAGGTCGAACAAAAGGGCTACAC
CCTGGTGCCGCTGAACCTGCACTGGAAAAATGGCGTCGTGAAATGCGAGATCGCGCTGGCCAGGGGCAAGGCCCAGCACG
ACAAGCGCAACTCCATCAAGGAGCGTGAAGGCAAGCGCGAGGTCGAGCGCGCGCTGAAAAGCCGCAGCCGTTGA

Upstream 100 bases:

>100_bases
CCGGCCGGGGGAAATTACAATCGCGCTGGCGCGGCGCTGAGCATTGTAGGCAGGCCGTCGATCTGCCCTGAACTGCCGGT
TTGCGCTGCATCCGAACCCC

Downstream 100 bases:

>100_bases
CCCTTGCGTCGAGCCTCGATCCGGCCGTCACTGCACCGGGCCGAAGGCACGGGTTCATCATGCGTCGGCGGCGAGCGCAT
CAGGAGCCGTCGGCGTCCGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTKKPDPASRIADNKKAAHDYFLEERYEAGMVLHGWEVKALRAGKAQLTDGYVLIRDGELFLLGCQLQALKTASTHVSPE
AARTKKLLLKKDDIRRLIGKVEQKGYTLVPLNLHWKNGVVKCEIALARGKAQHDKRNSIKEREGKREVERALKSRSR

Sequences:

>Translated_157_residues
MTKKPDPASRIADNKKAAHDYFLEERYEAGMVLHGWEVKALRAGKAQLTDGYVLIRDGELFLLGCQLQALKTASTHVSPE
AARTKKLLLKKDDIRRLIGKVEQKGYTLVPLNLHWKNGVVKCEIALARGKAQHDKRNSIKEREGKREVERALKSRSR
>Mature_156_residues
TKKPDPASRIADNKKAAHDYFLEERYEAGMVLHGWEVKALRAGKAQLTDGYVLIRDGELFLLGCQLQALKTASTHVSPEA
ARTKKLLLKKDDIRRLIGKVEQKGYTLVPLNLHWKNGVVKCEIALARGKAQHDKRNSIKEREGKREVERALKSRSR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=51.5527950310559, Blast_Score=165, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_VEREI (A1WPQ3)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_998628.1
- ProteinModelPortal:   A1WPQ3
- SMR:   A1WPQ3
- STRING:   A1WPQ3
- GeneID:   4695259
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_3903
- NMPDR:   fig|391735.5.peg.3576
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A1WPQ3
- ProtClustDB:   PRK05422
- BioCyc:   VEIS391735:VEIS_3903-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17813; Mature: 17682

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKPDPASRIADNKKAAHDYFLEERYEAGMVLHGWEVKALRAGKAQLTDGYVLIRDGEL
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCEEEEECCCE
FLLGCQLQALKTASTHVSPEAARTKKLLLKKDDIRRLIGKVEQKGYTLVPLNLHWKNGVV
EEECCCHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEECCCCEE
KCEIALARGKAQHDKRNSIKEREGKREVERALKSRSR
EEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKPDPASRIADNKKAAHDYFLEERYEAGMVLHGWEVKALRAGKAQLTDGYVLIRDGEL
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCEEEEECCCE
FLLGCQLQALKTASTHVSPEAARTKKLLLKKDDIRRLIGKVEQKGYTLVPLNLHWKNGVV
EEECCCHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEECCCCEE
KCEIALARGKAQHDKRNSIKEREGKREVERALKSRSR
EEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA