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The map label for this gene is mdeA [H]

Identifier: 121610703

GI number: 121610703

Start: 4171150

End: 4172460

Strand: Direct

Name: mdeA [H]

Synonym: Veis_3780

Alternate gene names: 121610703

Gene position: 4171150-4172460 (Clockwise)

Preceding gene: 121610689

Following gene: 121610704

Centisome position: 74.93

GC content: 68.88

Gene sequence:

>1311_bases
ATGCCCGGCTACTTCGACCCCGGTTTTGACACGCTGGCGCTGCACGCTGGCGCCAGCCCCGACCCGGCCACCGGCGCGCG
CGCCGTACCGCTTCACCTGAGCACCGCGTTCGTCTTCGCGTCGAGCGACCATGCCGCCGCGCTGTTCAACCTCGAACGCG
GCGGGCATGTGTACAGCCGCATCAGCAACCCGACCAACGCGGTGCTCGAGCAGCGCGTCGCCGCGCTCGAAGGCGGCGCC
GGCGCGATCGCCACCGCCAGCGGCCAGGCGGCGCTGCATCTGACGCTGGCCACGCTGATGGGCGCTGGCAGCCATATCGT
GGCCAGCAGCGCGCTGTATGGCGGCTCGCAGAACCTGCTGCACTACACGCTGCGCCGCTTTGGCATAGCGACCACGTTCG
TCAAGCCGGGCGACATCGACGCCTGGCGCGCGGCCGTGCGCCCCGACACCCGGCTGTTCTTTGGCGAGACGGTGGGCAAC
CCCGGTCTGGCCGTGCTCGACATCCCCACCGTGAGCGCCATCGCGCATGAGGCGGGCGTGCCGCTGCTGGTGGACTCCAC
CCTGACCTCGCCCTGGCTGATGCAGCCGTTCGAGCATGGCGCGGATCTGATCTGCCACTCGGCCACCAAATTCCTCTCGG
GCCATGGCACGGTGGTCGGCGGCATCGTGGTCGATGGCGGCGGCTTTGACTGGGACGGGCCCCGCTCGGCCGGCAAGTTT
GCCGAACTGACCCAGCCCTACGATGGCTTTCACGGCATGGTGTTTGGCGAAGAAAGCAGCGTCGGGGCCTTCCTGTTGCG
CGCGCGCCGCGAGGGCCTGCGCGACTTCGGCGCGTGCATGAGCCCGCATACCGCCTGGCTGATTCTGCAAGGCATCGAAA
CCCTGCCGCTGCGCATGGAGCGCCATATGCGCAACACCGCGAAGGTGGTGGAGTTTCTGGCCGCGCAGCCTTTCGTCGCG
CGCGTGGGCCACCCGATGCTGGCATCGCACCCCGACCATGCGCTGGCGCAAAAGCTGCTGCCGCGCGGCGCGGGGTCGGT
GTTCAGCTTTGACCTGAAGGGCAGCCGCGAGCAGGGCAAGAAATTCGTCGAAACTCTGAAGGTCTTCAGCCACCTGGCCA
ATGTCGGCGACTGCCGCAGCCTGGTGATCCACCCGGCCAGCACCACCCATTCGCGCATGGGTGCCCAGGCGCTGCAAGAC
GCCGGCATCACCCAGGGCCAGATCCGCCTGTCGATCGGGCTGGAAGACGCCGACGACCTGATCGACGACCTGCAGCGCGC
CCTCAGGGCCGCCGAGAAAGCAGGGGCCTGA

Upstream 100 bases:

>100_bases
AACGCCGCGATGCGCTTCGATGGCCAGCGCAGACCGACAGATGATCGGTGACGCGACACTAGTGCATTGTGGGCGATTAT
TGGCCCGCTCGGAGACAAAA

Downstream 100 bases:

>100_bases
CCATGCACATCGATGTCGACGGCCACCCGATCTATGGCTACACCGGCGGCAAGGCCCTGGTTGCGGCCCAGCCCACCGTG
GTCTTCATCCACGGCGTTCT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPGYFDPGFDTLALHAGASPDPATGARAVPLHLSTAFVFASSDHAAALFNLERGGHVYSRISNPTNAVLEQRVAALEGGA
GAIATASGQAALHLTLATLMGAGSHIVASSALYGGSQNLLHYTLRRFGIATTFVKPGDIDAWRAAVRPDTRLFFGETVGN
PGLAVLDIPTVSAIAHEAGVPLLVDSTLTSPWLMQPFEHGADLICHSATKFLSGHGTVVGGIVVDGGGFDWDGPRSAGKF
AELTQPYDGFHGMVFGEESSVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTAKVVEFLAAQPFVA
RVGHPMLASHPDHALAQKLLPRGAGSVFSFDLKGSREQGKKFVETLKVFSHLANVGDCRSLVIHPASTTHSRMGAQALQD
AGITQGQIRLSIGLEDADDLIDDLQRALRAAEKAGA

Sequences:

>Translated_436_residues
MPGYFDPGFDTLALHAGASPDPATGARAVPLHLSTAFVFASSDHAAALFNLERGGHVYSRISNPTNAVLEQRVAALEGGA
GAIATASGQAALHLTLATLMGAGSHIVASSALYGGSQNLLHYTLRRFGIATTFVKPGDIDAWRAAVRPDTRLFFGETVGN
PGLAVLDIPTVSAIAHEAGVPLLVDSTLTSPWLMQPFEHGADLICHSATKFLSGHGTVVGGIVVDGGGFDWDGPRSAGKF
AELTQPYDGFHGMVFGEESSVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTAKVVEFLAAQPFVA
RVGHPMLASHPDHALAQKLLPRGAGSVFSFDLKGSREQGKKFVETLKVFSHLANVGDCRSLVIHPASTTHSRMGAQALQD
AGITQGQIRLSIGLEDADDLIDDLQRALRAAEKAGA
>Mature_435_residues
PGYFDPGFDTLALHAGASPDPATGARAVPLHLSTAFVFASSDHAAALFNLERGGHVYSRISNPTNAVLEQRVAALEGGAG
AIATASGQAALHLTLATLMGAGSHIVASSALYGGSQNLLHYTLRRFGIATTFVKPGDIDAWRAAVRPDTRLFFGETVGNP
GLAVLDIPTVSAIAHEAGVPLLVDSTLTSPWLMQPFEHGADLICHSATKFLSGHGTVVGGIVVDGGGFDWDGPRSAGKFA
ELTQPYDGFHGMVFGEESSVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRMERHMRNTAKVVEFLAAQPFVAR
VGHPMLASHPDHALAQKLLPRGAGSVFSFDLKGSREQGKKFVETLKVFSHLANVGDCRSLVIHPASTTHSRMGAQALQDA
GITQGQIRLSIGLEDADDLIDDLQRALRAAEKAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=34.4262295081967, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=31.615925058548, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=30.5164319248826, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=33.806146572104, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=27.5700934579439, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=31.6176470588235, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=429, Percent_Identity=32.1678321678322, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=429, Percent_Identity=31.2354312354312, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=42.9885057471264, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=32.4825986078886, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=26, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=358, Percent_Identity=27.0949720670391, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=25.635103926097, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922554, Length=431, Percent_Identity=34.8027842227378, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46073; Mature: 45942

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGYFDPGFDTLALHAGASPDPATGARAVPLHLSTAFVFASSDHAAALFNLERGGHVYSR
CCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCHHHHH
ISNPTNAVLEQRVAALEGGAGAIATASGQAALHLTLATLMGAGSHIVASSALYGGSQNLL
CCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
HYTLRRFGIATTFVKPGDIDAWRAAVRPDTRLFFGETVGNPGLAVLDIPTVSAIAHEAGV
HHHHHHHCHHEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHCCC
PLLVDSTLTSPWLMQPFEHGADLICHSATKFLSGHGTVVGGIVVDGGGFDWDGPRSAGKF
CEEEECCCCCCHHHCHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCH
AELTQPYDGFHGMVFGEESSVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRME
HHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
RHMRNTAKVVEFLAAQPFVARVGHPMLASHPDHALAQKLLPRGAGSVFSFDLKGSREQGK
HHHHHHHHHHHHHHCCCHHHHCCCCHHCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHH
KFVETLKVFSHLANVGDCRSLVIHPASTTHSRMGAQALQDAGITQGQIRLSIGLEDADDL
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHH
IDDLQRALRAAEKAGA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGYFDPGFDTLALHAGASPDPATGARAVPLHLSTAFVFASSDHAAALFNLERGGHVYSR
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCHHHHH
ISNPTNAVLEQRVAALEGGAGAIATASGQAALHLTLATLMGAGSHIVASSALYGGSQNLL
CCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
HYTLRRFGIATTFVKPGDIDAWRAAVRPDTRLFFGETVGNPGLAVLDIPTVSAIAHEAGV
HHHHHHHCHHEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHCCC
PLLVDSTLTSPWLMQPFEHGADLICHSATKFLSGHGTVVGGIVVDGGGFDWDGPRSAGKF
CEEEECCCCCCHHHCHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCH
AELTQPYDGFHGMVFGEESSVGAFLLRARREGLRDFGACMSPHTAWLILQGIETLPLRME
HHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
RHMRNTAKVVEFLAAQPFVARVGHPMLASHPDHALAQKLLPRGAGSVFSFDLKGSREQGK
HHHHHHHHHHHHHHCCCHHHHCCCCHHCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHH
KFVETLKVFSHLANVGDCRSLVIHPASTTHSRMGAQALQDAGITQGQIRLSIGLEDADDL
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHH
IDDLQRALRAAEKAGA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]