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The map label for this gene is afr [H]

Identifier: 121610498

GI number: 121610498

Start: 3945612

End: 3946649

Strand: Direct

Name: afr [H]

Synonym: Veis_3569

Alternate gene names: 121610498

Gene position: 3945612-3946649 (Clockwise)

Preceding gene: 121610497

Following gene: 121610499

Centisome position: 70.88

GC content: 66.96

Gene sequence:

>1038_bases
ATGCCCCCGTCCAACCCACCATCCAACCCTCCCCGCCCGGCCATTGGCTGGGGGCTGATAGGGGCCAGCACCATTGCCGC
TGAACATATGCTCGGAGCCATCCGCGCCCAAGCCGGCCACGATGTCGTCGCCGTGATGAGCAGCAACGCCCGGCGCGGCC
GGGAATATGCCGAGAAGAACGCCATCGCGTCGGCTTGCGGGTCGCTGGACGAGTTGTTGCAGAACCCGGCGGTTCAGGCC
GTCTACATCAGCACCACCAATGAGTTGCACAAGGCGCAGGTGCTTGCCGCCGCCGCCGCCGGCAAGCATGTGCTGTGCGA
AAAGCCGTTGGCGCTCACGCTCGCTGATGCGCGCGAGATGGTGGCGGCCTGCGCTGGCGCAGGCGTGGTGATGGCGACCA
ACCACCACCTGCGCAACGCGGCAACGCATCGCAAAATTCGGGAACTGGTGGCCCAAGGGGCCATTGGCAAGCCCTTGTTC
GCCCGCGTGTTCCACGCGGTCTACCTGCCCGAGCATCTGCAGGGCTGGCGCCTGGACAGGCCCGAGGCCGGCGGCGGGGT
GATTCTGGACATCACCGTGCACGACGTGGACACCTTGCGCTTCGTGCTCGGTGCCGAGCCTGTCGATGCGGTCGGCCTGG
CGCAATCAGCGTCGCTGGCCAGGCATGGCCTGGAAGACGGGGTGATGGCCGTTTTGCGTTTCGACAACGGTGTGCTGGCG
CAAATTCATGATGCCTTCACCGTCAAACATGCCGGGACCGGCATCGAAATCCATGGCGCGACCGGCTCGATCATCGGCCG
CAATGTGATGACGCAGCGCCCGGTGGGCGAACTGGTGCTGCGCAATGCCGACGGCGAAACCCCGCTGGTCGTCGAGCACG
AGAACCTGTATGTGCGCGGCGTCGCCGCGTTTTGTGCCGCGCTGCGCGGGCAAGGCCAGCCCGCAGCCAGCGGCGCAGAT
GGTGTCCGTTCCCTGGCGGCGGCGCTGGCCGTGTTGCAGTCCTGCAAGACCGGGCAACGCATCGCCATCGCCCCGTGA

Upstream 100 bases:

>100_bases
GTGTTTACCCTGGTTTTCGGATTTTGCTGTTGACGGCGGCCTATGGGAACGTTCCAATGAATGCTTGCGATGCCGTTAGA
TCGTTCCAACAAGGTAGCAC

Downstream 100 bases:

>100_bases
CCTGCCCGTGGCATTCCTTGGCATTCCTTGACCTCCCTGGCGCCCCCGGGTGGTTGGGTTGATTTGGTTATTTGGCAGAT
TGAATCGCGAGACTGCGCAA

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MPPSNPPSNPPRPAIGWGLIGASTIAAEHMLGAIRAQAGHDVVAVMSSNARRGREYAEKNAIASACGSLDELLQNPAVQA
VYISTTNELHKAQVLAAAAAGKHVLCEKPLALTLADAREMVAACAGAGVVMATNHHLRNAATHRKIRELVAQGAIGKPLF
ARVFHAVYLPEHLQGWRLDRPEAGGGVILDITVHDVDTLRFVLGAEPVDAVGLAQSASLARHGLEDGVMAVLRFDNGVLA
QIHDAFTVKHAGTGIEIHGATGSIIGRNVMTQRPVGELVLRNADGETPLVVEHENLYVRGVAAFCAALRGQGQPAASGAD
GVRSLAAALAVLQSCKTGQRIAIAP

Sequences:

>Translated_345_residues
MPPSNPPSNPPRPAIGWGLIGASTIAAEHMLGAIRAQAGHDVVAVMSSNARRGREYAEKNAIASACGSLDELLQNPAVQA
VYISTTNELHKAQVLAAAAAGKHVLCEKPLALTLADAREMVAACAGAGVVMATNHHLRNAATHRKIRELVAQGAIGKPLF
ARVFHAVYLPEHLQGWRLDRPEAGGGVILDITVHDVDTLRFVLGAEPVDAVGLAQSASLARHGLEDGVMAVLRFDNGVLA
QIHDAFTVKHAGTGIEIHGATGSIIGRNVMTQRPVGELVLRNADGETPLVVEHENLYVRGVAAFCAALRGQGQPAASGAD
GVRSLAAALAVLQSCKTGQRIAIAP
>Mature_344_residues
PPSNPPSNPPRPAIGWGLIGASTIAAEHMLGAIRAQAGHDVVAVMSSNARRGREYAEKNAIASACGSLDELLQNPAVQAV
YISTTNELHKAQVLAAAAAGKHVLCEKPLALTLADAREMVAACAGAGVVMATNHHLRNAATHRKIRELVAQGAIGKPLFA
RVFHAVYLPEHLQGWRLDRPEAGGGVILDITVHDVDTLRFVLGAEPVDAVGLAQSASLARHGLEDGVMAVLRFDNGVLAQ
IHDAFTVKHAGTGIEIHGATGSIIGRNVMTQRPVGELVLRNADGETPLVVEHENLYVRGVAAFCAALRGQGQPAASGADG
VRSLAAALAVLQSCKTGQRIAIAP

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=287, Percent_Identity=29.616724738676, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787574, Length=360, Percent_Identity=26.6666666666667, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87082405, Length=284, Percent_Identity=27.8169014084507, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081947, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581117, Length=251, Percent_Identity=27.8884462151394, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581115, Length=196, Percent_Identity=29.5918367346939, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35926; Mature: 35794

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSNPPSNPPRPAIGWGLIGASTIAAEHMLGAIRAQAGHDVVAVMSSNARRGREYAEKN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
AIASACGSLDELLQNPAVQAVYISTTNELHKAQVLAAAAAGKHVLCEKPLALTLADAREM
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHH
VAACAGAGVVMATNHHLRNAATHRKIRELVAQGAIGKPLFARVFHAVYLPEHLQGWRLDR
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCC
PEAGGGVILDITVHDVDTLRFVLGAEPVDAVGLAQSASLARHGLEDGVMAVLRFDNGVLA
CCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCCCEE
QIHDAFTVKHAGTGIEIHGATGSIIGRNVMTQRPVGELVLRNADGETPLVVEHENLYVRG
EHHHHEEEEECCCCEEEECCCCCHHCCCHHCCCCHHHHEEECCCCCCCEEEECCCEEEHH
VAAFCAALRGQGQPAASGADGVRSLAAALAVLQSCKTGQRIAIAP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
PPSNPPSNPPRPAIGWGLIGASTIAAEHMLGAIRAQAGHDVVAVMSSNARRGREYAEKN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
AIASACGSLDELLQNPAVQAVYISTTNELHKAQVLAAAAAGKHVLCEKPLALTLADAREM
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHH
VAACAGAGVVMATNHHLRNAATHRKIRELVAQGAIGKPLFARVFHAVYLPEHLQGWRLDR
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCC
PEAGGGVILDITVHDVDTLRFVLGAEPVDAVGLAQSASLARHGLEDGVMAVLRFDNGVLA
CCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCCCEE
QIHDAFTVKHAGTGIEIHGATGSIIGRNVMTQRPVGELVLRNADGETPLVVEHENLYVRG
EHHHHEEEEECCCCEEEECCCCCHHCCCHHCCCCHHHHEEECCCCCCCEEEECCCEEEHH
VAAFCAALRGQGQPAASGADGVRSLAAALAVLQSCKTGQRIAIAP
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]