Click here to switch to the map view.

The map label for this gene is hsdM [H]

Identifier: 121609378

GI number: 121609378

Start: 2736727

End: 2738307

Strand: Direct

Name: hsdM [H]

Synonym: Veis_2421

Alternate gene names: 121609378

Gene position: 2736727-2738307 (Clockwise)

Preceding gene: 121609377

Following gene: 121609379

Centisome position: 49.16

GC content: 57.43

Gene sequence:

>1581_bases
ATGAATAACAGCAAAGAAACAGAGCGAGCAGAACTCCACAAAACTATCTGGCGTATCGCCAATGATCTGCGCGGGAGCGT
GGACGGCTGGGATTTCAAGACCTATGTGCTCGGCATGTTGTTCTACCGCTTTATCTCGGAGAACCTTACCGCCTACCTGA
ACGAGCAAGAACGCAACGCGGGAGATCCCGAATTTGAATACGCCGCGCTCAACGATTCTGGTGCCGAATTCGGTCGAGCC
GAAACTGTGGCAGAAAAGGGTTTCTACATCCTACCCTCACACCTCTTCGACAACGTGCGCAAGCAGGCCCGACTTGACGC
CAACCTCAATGAGACCCTCAGCCGAGTATTTGCCGACATCGAAAGCTCCGCTAACGGTGCGGACAGCGAGGACGACTTCA
AGGGTCTCTTCGACGACCTCGACGTCAACAGCAGCAAGCTCGGGCCTACGGTGGCCAAGCGCAACGAAAAGCTGGTGAAG
CTGCTCGACGCCATCGGTGACTTGCCCCTGACCAGCGCACCCGGCAAGTTTTCGGACAACACCATCGACCTTTTCGGCGA
TGCCTACGAATACCTGATGCAGATGTACGCCTCGACCGCTGGAAAATCCGGTGGAGAGTTCTACACCCCGCAAGAGGTCT
CCGAACTGCTGGCTCGCATCACCGTGGTTGGCAAAACCGAGATCAACAAGGTATACGACCCGGCCTGCGGCTCCGGCTCG
CTGTTGCTCAAGTTCGCCAAGGTACTGGGACACGATGCGGTGCGCCAGGGCTTCTTTGGCCAGGAAATCAACCTCACCAC
CTTCAACCTGTGCCGGATCAACATGTTCCTGCACGACGTGAATTACGAAAAATTCAACGTCGCCCACGGCGACACCCTCA
CCGACCCGGCCCACTGGGACGATGAACCCTTCGAGGCCATCGTCTCCAATCCGCCGTACTCGATCAGATGGGACGGCGAC
GCGAACCCCTTGCTCATCAACGACCCGCGCTTCGCGCCCGCTGGTGTGCTGGCACCCAAGAGCAAGGCCGACCTCGCCTT
CACCATGCACATTCTGAGCTGGCTGGCGGTCAACGGCACGGCGGCGATTGTTGAATTCCCCGGCGTGCTTTATCGCGGCG
GCGCAGAGCAGAAAATCCGGCAGTACCTGATCGATAACAACTACGTCGATACCGTTATCCAGTTGCCGCCTGATCTTTTC
TTTGGCACCACGATCGCGACCTGCATCATCGTGCTGAAAAAATCCAAGCGTGACAATGCCACGCTCTTTGTCGATGCCAG
CGCCGAGTTCATGCGCAGCGGCAACAAGAACAAACTGACAGACGCTCACCAGCAGAAGATTCTGGATGCCTACATCGAGC
GCAGAAACATAGATCACTTCGCCCGGCTGGTGGAAAACGGCGACATTGCAGAAAACGGCTACAACATCGCCGTCTCATCC
TATGTCGCGCAGGCCGATAACAGCGTGGCGGTGGATATCCAGGCGCTCAATGCCAAGATCGCCCGCATCGTGGGGCGGCA
GGCGGAGTTGCGAACGCAGATCGATGCCATCGTCGCGGACCTGGAAGGAGAAACGGCATGA

Upstream 100 bases:

>100_bases
TCAGTCAGCAAAGCGCCTGACACAACAACAGCTTCGCAGGGGCAGGCTTAATGAAACCCGTAAAGCAAAAAGATCAAAGT
AACTTGGAGGGGAATTGCTC

Downstream 100 bases:

>100_bases
GTAAACTGCCCCGCGGTGAAATAATCCTTTATCCGCGCGGCGATGGTTCGCCGTCCATTGAAGTTCATCTTGAAGGAGAC
ACTGTCTGGCTGACCCAAGA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNNSKETERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTAYLNEQERNAGDPEFEYAALNDSGAEFGRA
ETVAEKGFYILPSHLFDNVRKQARLDANLNETLSRVFADIESSANGADSEDDFKGLFDDLDVNSSKLGPTVAKRNEKLVK
LLDAIGDLPLTSAPGKFSDNTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSELLARITVVGKTEINKVYDPACGSGS
LLLKFAKVLGHDAVRQGFFGQEINLTTFNLCRINMFLHDVNYEKFNVAHGDTLTDPAHWDDEPFEAIVSNPPYSIRWDGD
ANPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDTVIQLPPDLF
FGTTIATCIIVLKKSKRDNATLFVDASAEFMRSGNKNKLTDAHQQKILDAYIERRNIDHFARLVENGDIAENGYNIAVSS
YVAQADNSVAVDIQALNAKIARIVGRQAELRTQIDAIVADLEGETA

Sequences:

>Translated_526_residues
MNNSKETERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTAYLNEQERNAGDPEFEYAALNDSGAEFGRA
ETVAEKGFYILPSHLFDNVRKQARLDANLNETLSRVFADIESSANGADSEDDFKGLFDDLDVNSSKLGPTVAKRNEKLVK
LLDAIGDLPLTSAPGKFSDNTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSELLARITVVGKTEINKVYDPACGSGS
LLLKFAKVLGHDAVRQGFFGQEINLTTFNLCRINMFLHDVNYEKFNVAHGDTLTDPAHWDDEPFEAIVSNPPYSIRWDGD
ANPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDTVIQLPPDLF
FGTTIATCIIVLKKSKRDNATLFVDASAEFMRSGNKNKLTDAHQQKILDAYIERRNIDHFARLVENGDIAENGYNIAVSS
YVAQADNSVAVDIQALNAKIARIVGRQAELRTQIDAIVADLEGETA
>Mature_526_residues
MNNSKETERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTAYLNEQERNAGDPEFEYAALNDSGAEFGRA
ETVAEKGFYILPSHLFDNVRKQARLDANLNETLSRVFADIESSANGADSEDDFKGLFDDLDVNSSKLGPTVAKRNEKLVK
LLDAIGDLPLTSAPGKFSDNTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSELLARITVVGKTEINKVYDPACGSGS
LLLKFAKVLGHDAVRQGFFGQEINLTTFNLCRINMFLHDVNYEKFNVAHGDTLTDPAHWDDEPFEAIVSNPPYSIRWDGD
ANPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDTVIQLPPDLF
FGTTIATCIIVLKKSKRDNATLFVDASAEFMRSGNKNKLTDAHQQKILDAYIERRNIDHFARLVENGDIAENGYNIAVSS
YVAQADNSVAVDIQALNAKIARIVGRQAELRTQIDAIVADLEGETA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=299, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58305; Mature: 58305

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSKETERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTAYLNEQERNA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
GDPEFEYAALNDSGAEFGRAETVAEKGFYILPSHLFDNVRKQARLDANLNETLSRVFADI
CCCCEEEEEECCCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ESSANGADSEDDFKGLFDDLDVNSSKLGPTVAKRNEKLVKLLDAIGDLPLTSAPGKFSDN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSELLARITVVGKTEINKVYDPACGSGS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCH
LLLKFAKVLGHDAVRQGFFGQEINLTTFNLCRINMFLHDVNYEKFNVAHGDTLTDPAHWD
HHHHHHHHHCHHHHHCCCCCCCCCCEEEEHEEEEEEHEECCCCEEEECCCCCCCCCCCCC
DEPFEAIVSNPPYSIRWDGDANPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGT
CCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
AAIVEFPGVLYRGGAEQKIRQYLIDNNYVDTVIQLPPDLFFGTTIATCIIVLKKSKRDNA
EEEEECCCCEECCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
TLFVDASAEFMRSGNKNKLTDAHQQKILDAYIERRNIDHFARLVENGDIAENGYNIAVSS
EEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHH
YVAQADNSVAVDIQALNAKIARIVGRQAELRTQIDAIVADLEGETA
HHHHCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNSKETERAELHKTIWRIANDLRGSVDGWDFKTYVLGMLFYRFISENLTAYLNEQERNA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
GDPEFEYAALNDSGAEFGRAETVAEKGFYILPSHLFDNVRKQARLDANLNETLSRVFADI
CCCCEEEEEECCCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ESSANGADSEDDFKGLFDDLDVNSSKLGPTVAKRNEKLVKLLDAIGDLPLTSAPGKFSDN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSELLARITVVGKTEINKVYDPACGSGS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCH
LLLKFAKVLGHDAVRQGFFGQEINLTTFNLCRINMFLHDVNYEKFNVAHGDTLTDPAHWD
HHHHHHHHHCHHHHHCCCCCCCCCCEEEEHEEEEEEHEECCCCEEEECCCCCCCCCCCCC
DEPFEAIVSNPPYSIRWDGDANPLLINDPRFAPAGVLAPKSKADLAFTMHILSWLAVNGT
CCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
AAIVEFPGVLYRGGAEQKIRQYLIDNNYVDTVIQLPPDLFFGTTIATCIIVLKKSKRDNA
EEEEECCCCEECCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
TLFVDASAEFMRSGNKNKLTDAHQQKILDAYIERRNIDHFARLVENGDIAENGYNIAVSS
EEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEHHH
YVAQADNSVAVDIQALNAKIARIVGRQAELRTQIDAIVADLEGETA
HHHHCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]