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The map label for this gene is mlaE [H]

Identifier: 121609355

GI number: 121609355

Start: 2716432

End: 2717214

Strand: Direct

Name: mlaE [H]

Synonym: Veis_2398

Alternate gene names: 121609355

Gene position: 2716432-2717214 (Clockwise)

Preceding gene: 121609354

Following gene: 121609356

Centisome position: 48.8

GC content: 67.82

Gene sequence:

>783_bases
ATGAACCGGCAGCCCATCGCCGACCTCGGCTTTGCCGTGCGCAGCAAACTGGCCGACATCGGCATGGCCGCGCGCTTGCT
GGCGCGCTTGCTGCTGCTGGCCGGAGCGGTTTGCCGGCGGCCCGCGCTGGTGCGCGACCAACTGCATTTCCTGGGCAACT
ACTCGCTGGCCATCATTGTGGTCTCCGGCCTGTTCGTCGGCTTTGTGCTCGGCCTGCAGGGCTACTACACCTTGCAGCGC
TATGGCGCGTCAGAGGCGCTCGGGCTGCTGGTGGCGCTGTCGCTGGTGCGCGAACTCGGGCCGGTGGTCACGGCGCTGCT
GTTTGCCGGCCGCGCAGGCACCTCGCTGACGGCCGAGATCGGCCTGATGCGCTCGGGCGAGCAATTGAGCGCCATGGAAA
TGATGGCCATCGACCCGGTGCGCCGCATCCTGGCGCCCCGATTCTGGGCCGGGGTCATCGCCATGCCGCTGCTGGCAGCG
ATGTTCAGCGCCGTCGGCGTGATCGGCGGCGCCGTGGTGGGCGTGCTGATGATAGGCATAGACCCGGGGGCCTTCTGGAG
CCAGATGCAAGGCGGCGTGGATATCTGGCGCGACCTGGGCAATGGGGTGCTCAAGAGCCTGGTCTTCGGCTTTACCGTAA
GCTTTGTGGCGCTGCTGCAAGGCTATGTGGCCAGGCCCACGCCCGAAGGCGTGGCGCGCGCCACCACGCGCACGGTGGTG
CTGGCCTCGCTGGCCGTGCTGGCGCTGGACTTCATCCTCACGGCCCTGATGTTCAGTTGGTGA

Upstream 100 bases:

>100_bases
TGCAGCAATTCGTCAACGCCTTGCCGACCGGGCCGGTGCCGTTCCACTACCCGGGCCCGGGGTTGGCCGAGGATTTCGGC
CCCGTCGCAGGGGGCGCGCG

Downstream 100 bases:

>100_bases
GACACTGGCAAGTGCAGAAAGCAACGACAAATGCAACGCTCGAAAAATGATCTGTGGGTAGGCCTGTTCGTACTTCTGGG
GGTGCTGGCGCTGGTATTTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNRQPIADLGFAVRSKLADIGMAARLLARLLLLAGAVCRRPALVRDQLHFLGNYSLAIIVVSGLFVGFVLGLQGYYTLQR
YGASEALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMRSGEQLSAMEMMAIDPVRRILAPRFWAGVIAMPLLAA
MFSAVGVIGGAVVGVLMIGIDPGAFWSQMQGGVDIWRDLGNGVLKSLVFGFTVSFVALLQGYVARPTPEGVARATTRTVV
LASLAVLALDFILTALMFSW

Sequences:

>Translated_260_residues
MNRQPIADLGFAVRSKLADIGMAARLLARLLLLAGAVCRRPALVRDQLHFLGNYSLAIIVVSGLFVGFVLGLQGYYTLQR
YGASEALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMRSGEQLSAMEMMAIDPVRRILAPRFWAGVIAMPLLAA
MFSAVGVIGGAVVGVLMIGIDPGAFWSQMQGGVDIWRDLGNGVLKSLVFGFTVSFVALLQGYVARPTPEGVARATTRTVV
LASLAVLALDFILTALMFSW
>Mature_260_residues
MNRQPIADLGFAVRSKLADIGMAARLLARLLLLAGAVCRRPALVRDQLHFLGNYSLAIIVVSGLFVGFVLGLQGYYTLQR
YGASEALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMRSGEQLSAMEMMAIDPVRRILAPRFWAGVIAMPLLAA
MFSAVGVIGGAVVGVLMIGIDPGAFWSQMQGGVDIWRDLGNGVLKSLVFGFTVSFVALLQGYVARPTPEGVARATTRTVV
LASLAVLALDFILTALMFSW

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=219, Percent_Identity=65.296803652968, Blast_Score=270, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27552; Mature: 27552

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]