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The map label for this gene is pflA

Identifier: 121639021

GI number: 121639021

Start: 3457242

End: 3458330

Strand: Direct

Name: pflA

Synonym: BCG_3161

Alternate gene names: NA

Gene position: 3457242-3458330 (Clockwise)

Preceding gene: 121639020

Following gene: 121639022

Centisome position: 79.03

GC content: 61.89

Gene sequence:

>1089_bases
ATGAGCGACCCGTTCACCATCGCAACCAAACACTGGCACCGACTGCACGACAGCCGGATCCAGTGCGATGTATGTCCACG
CGCATGCAAACTTCACGAGGGACAGCGTGGCCTGTGTTTCGTCCGCGGCCGATTTGACGATCAAGTGAAGCTCACCAGCT
ACGGACGCTCTAGCGGATTCTGTGTCGATCCGATCGAGAAAAAGCCGCTCAACCACTTCTTGCCAGGTTCGGCGACGCTG
TCTTTCGGCACCGCCGGGTGCAACCTGGCGTGCAAGTTCTGCCAGAACTGGGATATCTCCAAGTCCCGCGAGATCGACGT
CCTGGCCAATCGGGCGGCCCCGGCCGACATCGCCCGGACCGCACACGAATTGGGTTGCCGCAGCGTGGCATTCACCTACA
ACGACCCAACGATCTTCTGGGAGTATGCCGCCGATGTAGCCGACGCCTGCCACGACCAGGGAATCAAAGCCGTCGCGGTG
ACGGCCGGGTACATGTGTCCTGAGCCCCGCGCGGAATTCTACCGGCGTGTCGACGCCGCCAACGTCGACCTAAAGGCATT
CACCGAAGACTTTTATCGCAAGGTTTGCGTCAGTCACCTGCGCAACGTCCTGGACACCCTGGCCTACCTGCGGCACCAGA
CGAATGTGTGGTTGGAGATCACCACCCTGCTGATTCCCGGACGTAACGACAGCGACGCGGAAGTCGCTGCCGAATGCAGA
TGGATCCGCGAAAACCTGGGCGTCGACGTGCCGGTGCATTTCACCGCGTTCCATCCCGACTACAAGATGATGGACACCCC
GGCTACACCACCCGCCACATTGACCCGAGCCCGCGAGATCGGCATTGGCGAAGGCCTGCGCTTCGTCTACACCGGAAACG
TTCACGATGCCGTGGGTGGCAGCACCTCGTGCCCAGGCTGCCGGGCAACGGTGATCGTTCGCGACTGGTATTCGATACGA
CATTACGCCCTCACCGAGGACGGCCGCTGCCAAGCATGCGGCTATCAGATGCCTGGCGTGTACGACGGACCGGCCGGACA
CTGGGGCCAGCGCCGGCTGCCCTTGCTGACCAGCTTGTCCCGGATGTGA

Upstream 100 bases:

>100_bases
GCCGGCCCACACGGGGGCAGCGCCGTTGCAACCAACGGTCTGTTGCACGACGAGGTGCTGACACGGCTCAACGCCGGGTA
ACCTGGCGCTCGAGAGCGCC

Downstream 100 bases:

>100_bases
ACAACTTAACAAGCACCCCTATCTTACTCCGGAGTAAGATAGGGTGGTCCGCTATCACCCCGATGACCGAGGCTGCCGTA
TGACCAACACCACCTCTGCT

Product: putative pyruvate formate lyase activating protein pflA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGFCVDPIEKKPLNHFLPGSATL
SFGTAGCNLACKFCQNWDISKSREIDVLANRAAPADIARTAHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAV
TAGYMCPEPRAEFYRRVDAANVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECR
WIRENLGVDVPVHFTAFHPDYKMMDTPATPPATLTRAREIGIGEGLRFVYTGNVHDAVGGSTSCPGCRATVIVRDWYSIR
HYALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLSRM

Sequences:

>Translated_362_residues
MSDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGFCVDPIEKKPLNHFLPGSATL
SFGTAGCNLACKFCQNWDISKSREIDVLANRAAPADIARTAHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAV
TAGYMCPEPRAEFYRRVDAANVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECR
WIRENLGVDVPVHFTAFHPDYKMMDTPATPPATLTRAREIGIGEGLRFVYTGNVHDAVGGSTSCPGCRATVIVRDWYSIR
HYALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLSRM
>Mature_361_residues
SDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGFCVDPIEKKPLNHFLPGSATLS
FGTAGCNLACKFCQNWDISKSREIDVLANRAAPADIARTAHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAVT
AGYMCPEPRAEFYRRVDAANVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECRW
IRENLGVDVPVHFTAFHPDYKMMDTPATPPATLTRAREIGIGEGLRFVYTGNVHDAVGGSTSCPGCRATVIVRDWYSIRH
YALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLSRM

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR016431
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40456; Mature: 40325

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGF
CCCCEEEHHHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCC
CVDPIEKKPLNHFLPGSATLSFGTAGCNLACKFCQNWDISKSREIDVLANRAAPADIART
EECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
AHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAVTAGYMCPEPRAEFYRRVDAA
HHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCC
NVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHH
WIRENLGVDVPVHFTAFHPDYKMMDTPATPPATLTRAREIGIGEGLRFVYTGNVHDAVGG
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHCCCC
STSCPGCRATVIVRDWYSIRHYALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLS
CCCCCCCEEEEEEECCHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
RM
CC
>Mature Secondary Structure 
SDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGF
CCCEEEHHHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCC
CVDPIEKKPLNHFLPGSATLSFGTAGCNLACKFCQNWDISKSREIDVLANRAAPADIART
EECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
AHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAVTAGYMCPEPRAEFYRRVDAA
HHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCC
NVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHH
WIRENLGVDVPVHFTAFHPDYKMMDTPATPPATLTRAREIGIGEGLRFVYTGNVHDAVGG
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHCCCC
STSCPGCRATVIVRDWYSIRHYALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLS
CCCCCCCEEEEEEECCHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
RM
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]