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The map label for this gene is lipI [H]

Identifier: 121637330

GI number: 121637330

Start: 1600622

End: 1601584

Strand: Reverse

Name: lipI [H]

Synonym: BCG_1461c

Alternate gene names: 121637330

Gene position: 1601584-1600622 (Counterclockwise)

Preceding gene: 121637333

Following gene: 121637329

Centisome position: 36.61

GC content: 67.39

Gene sequence:

>963_bases
ATGCCCAGTTTGGACAACACCGCCGACGAGAAACCCGCGATCGACCCCATCCTGCTGAAGGTACTGGATGCGGTTCCGTT
TCGGCTATCGATCGACGATGGGATCGAGGCCGTGCGCCAGCGGTTGCGCGATCTACCGCGCCAGCCGGTGCACCCCGAGC
TGCGGGTCGTTGACCTTGCGATCGATGGACCGGCGGGGCCGATCGGGACCCGGATCTATTGGCCGCCAACGTGTCCCGAT
CAAGCCGAGGCGCCCGTTGTGCTGTATTTCCATGGCGGCGGATTCGTCATGGGCGACCTCGACACGCACGATGGCACCTG
CCGCCAGCACGCCGTCGGCGCTGACGCGATTGTCGTGTCCGTCGACTACCGGCTGGCGCCTGAGCACCCCTACCCCGCTG
CCATCGAAGATGCTTGGGCCGCAACGCGGTGGGTCGCCGAGCATGGCCGCCAGGTCGGTGCTGACCTCGGCCGCATCGCC
GTCGCCGGAGATTCCGCCGGCGGCACCATCGCCGCGGTGATCGCGCAGCGAGCCCGCGATATGGGCGGCCCGCCCATTGT
GTTTCAGCTGTTGTGGTATCCCTCCACTCTGTGGGACCAATCGCTGCCGTCGTTGGCCGAGAATGCCGACGCACCGATCC
TCGACGTCAAGGCAATTGCCGCGTTCTCCCGTTGGTACGCAGGCGAAATCGACTTGCACAACCCGCCAGCGCCAATGGCG
CCCGGCCGAGCGGAGAACCTGGCCGACCTGCCGCCGGCCTACATCGCTGTCGCCGGCTACGACCCTTTGCGCGACGACGG
GATTCGGTACGGCGAGCTGCTGGCCGCCGCCGGTGTTCCCGTCGAGGTGCACAACGCCCAGACGCTGGTGCACGGCTACG
TTGGCTATGCCGGTGTGGTGCCCGCCGCCACCGAGGCCACCAACCGTGGGCTGGTGGCGCTACGGGTTGTGCTACACGGA
TAG

Upstream 100 bases:

>100_bases
GCGCAATGCGATCACGGTCAAGTACACGCCGTAGGCGAGGCCCGCCCAGCCGGCGACCACCCAGCCGCCGGCCAGCAAGC
GAGGTGCGTACGGTACCGGT

Downstream 100 bases:

>100_bases
GCCTGGCGGTCGTACGGTAGGCCCATGACAGAGCCGACCGTCGCCCGGCCTGATATCGATCCCGTGCTGAAGATGCTGCT
CGATACCTTTCCGGTGACCT

Product: putative lipase lipH

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRDLPRQPVHPELRVVDLAIDGPAGPIGTRIYWPPTCPD
QAEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIA
VAGDSAGGTIAAVIAQRARDMGGPPIVFQLLWYPSTLWDQSLPSLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMA
PGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVVPAATEATNRGLVALRVVLHG

Sequences:

>Translated_320_residues
MPSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRDLPRQPVHPELRVVDLAIDGPAGPIGTRIYWPPTCPD
QAEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIA
VAGDSAGGTIAAVIAQRARDMGGPPIVFQLLWYPSTLWDQSLPSLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMA
PGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVVPAATEATNRGLVALRVVLHG
>Mature_319_residues
PSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRDLPRQPVHPELRVVDLAIDGPAGPIGTRIYWPPTCPDQ
AEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAV
AGDSAGGTIAAVIAQRARDMGGPPIVFQLLWYPSTLWDQSLPSLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMAP
GRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVVPAATEATNRGLVALRVVLHG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=311, Percent_Identity=27.9742765273312, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI206597554, Length=310, Percent_Identity=29.3548387096774, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI68299767, Length=296, Percent_Identity=29.0540540540541, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI68051721, Length=305, Percent_Identity=25.5737704918033, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI226423947, Length=313, Percent_Identity=24.9201277955272, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI61966717, Length=317, Percent_Identity=26.1829652996845, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI21328446, Length=158, Percent_Identity=33.5443037974684, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI157041237, Length=251, Percent_Identity=26.2948207171315, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1786682, Length=262, Percent_Identity=28.6259541984733, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=263, Percent_Identity=29.277566539924, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=124, Percent_Identity=37.9032258064516, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=124, Percent_Identity=37.0967741935484, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=143, Percent_Identity=33.5664335664336, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115533412, Length=123, Percent_Identity=32.520325203252, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=123, Percent_Identity=32.520325203252, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24656084, Length=97, Percent_Identity=35.0515463917526, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24656076, Length=97, Percent_Identity=35.0515463917526, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130169, Length=97, Percent_Identity=35.0515463917526, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34053; Mature: 33922

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRDLPRQPVHPELRVVDLA
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
IDGPAGPIGTRIYWPPTCPDQAEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVS
ECCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHCCCCCEEEEE
VDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAVAGDSAGGTIAAVIAQRARD
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
MGGPPIVFQLLWYPSTLWDQSLPSLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMA
CCCCCEEEHEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCEEECCCCCCCCC
PGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHCCC
PAATEATNRGLVALRVVLHG
CCCHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
PSLDNTADEKPAIDPILLKVLDAVPFRLSIDDGIEAVRQRLRDLPRQPVHPELRVVDLA
CCCCCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
IDGPAGPIGTRIYWPPTCPDQAEAPVVLYFHGGGFVMGDLDTHDGTCRQHAVGADAIVVS
ECCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHCCCCCEEEEE
VDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAVAGDSAGGTIAAVIAQRARD
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
MGGPPIVFQLLWYPSTLWDQSLPSLAENADAPILDVKAIAAFSRWYAGEIDLHNPPAPMA
CCCCCEEEHEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCEEECCCCCCCCC
PGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQTLVHGYVGYAGVV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHCCC
PAATEATNRGLVALRVVLHG
CCCHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]