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The map label for this gene is folP2

Identifier: 121637136

GI number: 121637136

Start: 1380910

End: 1381866

Strand: Direct

Name: folP2

Synonym: BCG_1267

Alternate gene names: 121637136

Gene position: 1380910-1381866 (Clockwise)

Preceding gene: 121637135

Following gene: 121637137

Centisome position: 31.57

GC content: 68.03

Gene sequence:

>957_bases
GTGCGTTCAACACCGCCGGCCTCAGCCGGTCGTTCAACACCGCCGGCGTTAGCCGGCCATTCAACACCGCCGGCGTTAGC
CGGCCATTCAACGCTGTGCGGCCGTCCAGTCGCAGGTGATCGTGCGCTGATCATGGCGATCGTCAACCGCACCCCGGATT
CGTTTTACGACAAGGGTGCGACTTTCAGCGACGCGGCTGCCAGAGACGCGGTCCACCGGGCCGTCGCCGACGGTGCCGAC
GTCATCGACGTCGGCGGTGTCAAAGCCGGCCCGGGTGAACGCGTCGACGTCGACACCGAGATCACGCGGCTGGTGCCGTT
CATCGAATGGCTCCGCGGTGCTTACCCGGACCAGCTGATCAGTGTCGACACCTGGCGCGCGCAGGTGGCGAAGGCGGCCT
GCGCGGCGGGGGCGGACCTGATCAACGACACCTGGGGTGGCGTCGACCCGGCCATGCCCGAGGTGGCCGCCGAGTTCGGC
GCGGGCCTGGTGTGTGCGCACACCGGCGGCGCGCTGCCACGCACGCGACCCTTCCGGGTGAGCTACGGTACGACTACCCG
CGGTGTGGTGGATGCTGTGATTAGCCAGGTCACAGCCGCCGCCGAGCGGGCCGTCGCGGCCGGGGTGGCCCGCGAGAAGG
TGTTGATCGACCCGGCACACGACTTCGGCAAGAACACCTTCCATGGGCTGCTGCTATTGCGACACGTGGCCGATCTTGTT
ATGACCGGGTGGCCCGTGCTGATGGCTTTGAGCAACAAGGACGTTGTCGGGGAGACTCTGGGCGTGGATTTGACCGAACG
GCTTGAGGGAACGCTGGCAGCCACCGCGTTGGCTGCGGCCGCCGGGGCGCGCATGTTTCGGGTGCATGAGGTCGCCGCCA
CCCGGCGGGTGCTGGAAATGGTGGCATCGATTCAGGGGGTCCGGCCGCCGACGCGCACGGTGAGAGGACTCGCATGA

Upstream 100 bases:

>100_bases
AGGAGGTTTCGCTCGGAAGGCGACCGCAGGGCTAGCGGATTCCGGGCGCAGTCTCGATACCCGCACTGGACGCTCGACGG
TGACCAGGCACTATGGATGC

Downstream 100 bases:

>100_bases
CAGCATCGGAGCTGGTCGCCGGCGATCTCGCCGGTGGCAGGGCCCCTGGCGCGCTGCCCTTGGACACTACTTGGCACCGT
CCCGGCTGGACGATCGGGGA

Product: putative dihydropteroate synthase 2 folP2

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRSTPPASAGRSTPPALAGHSTPPALAGHSTLCGRPVAGDRALIMAIVNRTPDSFYDKGATFSDAAARDAVHRAVADGAD
VIDVGGVKAGPGERVDVDTEITRLVPFIEWLRGAYPDQLISVDTWRAQVAKAACAAGADLINDTWGGVDPAMPEVAAEFG
AGLVCAHTGGALPRTRPFRVSYGTTTRGVVDAVISQVTAAAERAVAAGVAREKVLIDPAHDFGKNTFHGLLLLRHVADLV
MTGWPVLMALSNKDVVGETLGVDLTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEMVASIQGVRPPTRTVRGLA

Sequences:

>Translated_318_residues
MRSTPPASAGRSTPPALAGHSTPPALAGHSTLCGRPVAGDRALIMAIVNRTPDSFYDKGATFSDAAARDAVHRAVADGAD
VIDVGGVKAGPGERVDVDTEITRLVPFIEWLRGAYPDQLISVDTWRAQVAKAACAAGADLINDTWGGVDPAMPEVAAEFG
AGLVCAHTGGALPRTRPFRVSYGTTTRGVVDAVISQVTAAAERAVAAGVAREKVLIDPAHDFGKNTFHGLLLLRHVADLV
MTGWPVLMALSNKDVVGETLGVDLTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEMVASIQGVRPPTRTVRGLA
>Mature_318_residues
MRSTPPASAGRSTPPALAGHSTPPALAGHSTLCGRPVAGDRALIMAIVNRTPDSFYDKGATFSDAAARDAVHRAVADGAD
VIDVGGVKAGPGERVDVDTEITRLVPFIEWLRGAYPDQLISVDTWRAQVAKAACAAGADLINDTWGGVDPAMPEVAAEFG
AGLVCAHTGGALPRTRPFRVSYGTTTRGVVDAVISQVTAAAERAVAAGVAREKVLIDPAHDFGKNTFHGLLLLRHVADLV
MTGWPVLMALSNKDVVGETLGVDLTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEMVASIQGVRPPTRTVRGLA

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain

Homologues:

Organism=Escherichia coli, GI87082224, Length=270, Percent_Identity=34.4444444444444, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324073, Length=319, Percent_Identity=29.4670846394984, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHPS2_MYCBO (P64140)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854893.1
- ProteinModelPortal:   P64140
- SMR:   P64140
- EnsemblBacteria:   EBMYCT00000015850
- GeneID:   1090528
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1239
- GeneTree:   EBGT00050000015914
- HOGENOM:   HBG754410
- OMA:   PRTRPHR
- ProtClustDB:   CLSK871906
- BioCyc:   MBOV233413:MB1239-MONOMER
- BRENDA:   2.5.1.15
- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489
- Gene3D:   G3DSA:3.20.20.20
- PANTHER:   PTHR20941:SF1
- TIGRFAMs:   TIGR01496

Pfam domain/function: PF00809 Pterin_bind; SSF51717 DHP_synth_like

EC number: 2.5.1.15

Molecular weight: Translated: 33046; Mature: 33046

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00792 DHPS_1; PS00793 DHPS_2; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTPPASAGRSTPPALAGHSTPPALAGHSTLCGRPVAGDRALIMAIVNRTPDSFYDKGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TFSDAAARDAVHRAVADGADVIDVGGVKAGPGERVDVDTEITRLVPFIEWLRGAYPDQLI
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHE
SVDTWRAQVAKAACAAGADLINDTWGGVDPAMPEVAAEFGAGLVCAHTGGALPRTRPFRV
EHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEE
SYGTTTRGVVDAVISQVTAAAERAVAAGVAREKVLIDPAHDFGKNTFHGLLLLRHVADLV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHCCHHHHHHHHHHHHHHHH
MTGWPVLMALSNKDVVGETLGVDLTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEM
HCCCHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASIQGVRPPTRTVRGLA
HHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MRSTPPASAGRSTPPALAGHSTPPALAGHSTLCGRPVAGDRALIMAIVNRTPDSFYDKGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCC
TFSDAAARDAVHRAVADGADVIDVGGVKAGPGERVDVDTEITRLVPFIEWLRGAYPDQLI
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHE
SVDTWRAQVAKAACAAGADLINDTWGGVDPAMPEVAAEFGAGLVCAHTGGALPRTRPFRV
EHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEE
SYGTTTRGVVDAVISQVTAAAERAVAAGVAREKVLIDPAHDFGKNTFHGLLLLRHVADLV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHCCHHHHHHHHHHHHHHHH
MTGWPVLMALSNKDVVGETLGVDLTERLEGTLAATALAAAAGARMFRVHEVAATRRVLEM
HCCCHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASIQGVRPPTRTVRGLA
HHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972