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The map label for this gene is ispH

Identifier: 121637041

GI number: 121637041

Start: 1267600

End: 1268607

Strand: Direct

Name: ispH

Synonym: BCG_1170

Alternate gene names: 121637041

Gene position: 1267600-1268607 (Clockwise)

Preceding gene: 121637036

Following gene: 121637043

Centisome position: 28.98

GC content: 65.87

Gene sequence:

>1008_bases
ATGGTTCCGACGGTCGACATGGGGATTCCCGGGGCTTCGGTATCGTCGCGATCGGTGGCCGACCGTCCCAACCGTAAGCG
GGTGCTGCTGGCCGAGCCGCGTGGCTACTGCGCTGGCGTGGATCGGGCCGTCGAAACGGTCGAACGCGCGCTTCAAAAAC
ACGGCCCGCCTGTCTACGTGCGTCACGAGATCGTGCATAACCGCCACGTGGTTGACACCCTGGCTAAGGCCGGTGCGGTT
TTCGTCGAAGAGACCGAGCAGGTTCCCGAGGGAGCGATTGTGGTGTTCTCCGCGCACGGGGTCGCGCCTACGGTGCACGT
CAGCGCCAGCGAGCGCAACCTGCAGGTCATTGACGCCACCTGCCCGCTGGTCACCAAGGTGCACAACGAGGCCAGGCGGT
TCGCCCGGGACGACTACGACATCTTGCTGATCGGTCATGAGGGCCACGAGGAAGTCGTCGGTACTGCTGGGGAAGCTCCC
GATCATGTGCAGCTGGTCGACGGGGTGGACGCCGTCGACCAGGTGACCGTCCGTGACGAGGACAAAGTGGTTTGGCTGTC
GCAGACCACCCTGTCCGTCGATGAGACCATGGAGATTGTCGGGCGGTTGCGTCGGCGTTTCCCCAAGCTGCAGGATCCGC
CCAGCGACGACATCTGCTATGCGACCCAGAATCGGCAGGTCGCGGTCAAGGCGATGGCGCCCGAGTGCGAGCTGGTCATC
GTGGTCGGCTCGCGCAATTCGTCGAATTCGGTTCGGCTGGTCGAGGTGGCGCTGGGTGCCGGGGCGCGGGCCGCCCACCT
GGTGGACTGGGCCGACGATATCGACTCGGCCTGGCTGGACGGCGTTACCACGGTCGGCGTTACGTCGGGGGCATCGGTCC
CCGAGGTGCTGGTGCGCGGTGTGCTGGAGCGGCTGGCCGAATGCGGCTACGACATCGTGCAACCGGTGACAACGGCCAAC
GAGACGTTGGTGTTCGCATTGCCCCGGGAGCTCCGCTCACCTCGCTGA

Upstream 100 bases:

>100_bases
GCAGTAGCCATGGATCCAAGACTGCCTCAAGCAGCGGCTAACTCCAAGCCGGTGGCCGTGAGCTGGCGGGTTCGTGTCGG
CCCAAAGTACCCTGAATGCC

Downstream 100 bases:

>100_bases
GCACATCCGCTCACGGTTAGACGTCGTATTCCCAGGATTCAGCCGGTGGTCTGCGCGGTGCCCGCGAACGATCCCGCCGA
TCGAACCGCTGCTCCTCGCG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYVRHEIVHNRHVVDTLAKAGAV
FVEETEQVPEGAIVVFSAHGVAPTVHVSASERNLQVIDATCPLVTKVHNEARRFARDDYDILLIGHEGHEEVVGTAGEAP
DHVQLVDGVDAVDQVTVRDEDKVVWLSQTTLSVDETMEIVGRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVALGAGARAAHLVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRGVLERLAECGYDIVQPVTTAN
ETLVFALPRELRSPR

Sequences:

>Translated_335_residues
MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYVRHEIVHNRHVVDTLAKAGAV
FVEETEQVPEGAIVVFSAHGVAPTVHVSASERNLQVIDATCPLVTKVHNEARRFARDDYDILLIGHEGHEEVVGTAGEAP
DHVQLVDGVDAVDQVTVRDEDKVVWLSQTTLSVDETMEIVGRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVALGAGARAAHLVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRGVLERLAECGYDIVQPVTTAN
ETLVFALPRELRSPR
>Mature_335_residues
MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYVRHEIVHNRHVVDTLAKAGAV
FVEETEQVPEGAIVVFSAHGVAPTVHVSASERNLQVIDATCPLVTKVHNEARRFARDDYDILLIGHEGHEEVVGTAGEAP
DHVQLVDGVDAVDQVTVRDEDKVVWLSQTTLSVDETMEIVGRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVALGAGARAAHLVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRGVLERLAECGYDIVQPVTTAN
ETLVFALPRELRSPR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=52.1035598705502, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH1_MYCBO (P0A5I1)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854796.1
- EnsemblBacteria:   EBMYCT00000017881
- GeneID:   1090413
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1140
- GeneTree:   EBGT00050000016858
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   MBOV233413:MB1140-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36297; Mature: 36297

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RHEIVHNRHVVDTLAKAGAVFVEETEQVPEGAIVVFSAHGVAPTVHVSASERNLQVIDAT
EHHHHHCCHHHHHHHHCCEEEEECHHCCCCCEEEEEEECCCCEEEEEECCCCCEEEEECC
CPLVTKVHNEARRFARDDYDILLIGHEGHEEVVGTAGEAPDHVQLVDGVDAVDQVTVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCEEEECCCCCHHEEEECCC
DKVVWLSQTTLSVDETMEIVGRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
CCEEEEEECEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEE
VVGSRNSSNSVRLVEVALGAGARAAHLVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRG
EECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
VLERLAECGYDIVQPVTTANETLVFALPRELRSPR
HHHHHHHCCHHHHCCCCCCCCEEEEEECHHHCCCC
>Mature Secondary Structure
MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RHEIVHNRHVVDTLAKAGAVFVEETEQVPEGAIVVFSAHGVAPTVHVSASERNLQVIDAT
EHHHHHCCHHHHHHHHCCEEEEECHHCCCCCEEEEEEECCCCEEEEEECCCCCEEEEECC
CPLVTKVHNEARRFARDDYDILLIGHEGHEEVVGTAGEAPDHVQLVDGVDAVDQVTVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCEEEECCCCCHHEEEECCC
DKVVWLSQTTLSVDETMEIVGRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
CCEEEEEECEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEE
VVGSRNSSNSVRLVEVALGAGARAAHLVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRG
EECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
VLERLAECGYDIVQPVTTANETLVFALPRELRSPR
HHHHHHHCCHHHHCCCCCCCCEEEEEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972