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The map label for this gene is pnbA [H]

Identifier: 121637036

GI number: 121637036

Start: 1263726

End: 1264241

Strand: Direct

Name: pnbA [H]

Synonym: BCG_1165

Alternate gene names: 121637036

Gene position: 1263726-1264241 (Clockwise)

Preceding gene: 121637035

Following gene: 121637041

Centisome position: 28.89

GC content: 63.76

Gene sequence:

>516_bases
ATGTTCACCCAGATTGCCGCCGAACAGCCCGATCTGCAAGTGCCAACCGAGGAGCAGATCGGCTCCGCGTACTCGCGATG
GCGGCGCAAAGCACGCTCATTGAGTATGGCTACCGACGTCGGCTTCCGGATGCCGTCGGTGTGGCTCGCTGAAGGGCACA
GCGGGGTGGCGCCGGTGTATCTGTATCGGTTTGACTACTCGACTCCGCTGCTGAAGCTGCTGCTGGTCCGGGCCGCCCAT
GCCACCGAATTGCCTTACGTCTGGGGCAATCTCGGAGGATCCCAGGACCCTGCATTGAAGTTGGGCGACGCCAAAGCCGC
CATAGCGGTGTCCCGGAGGGTACGGACGCGGTGGATCAATTTCGCGACGCGGGGCAAACCCACGGGTCCCGATGGCGAGC
CAGACTGGCCATGTTACGAGGAGGCCCATCGTGCCTGCCTGATTATCGGCAGGCGAGACGCCGTCGTGCACGACGTCGAC
GCACACATCCGAGCGACCTGGGGCAGCAAGTGGTGA

Upstream 100 bases:

>100_bases
CACCCGGTTCCCTTGATCATCGGCACCAACAAGCACGAGTCGGCGCTCTTTCGGTTGATGCGCTCGCCGCTGATGCCGAT
CACCCCGCGCGATCACGTCG

Downstream 100 bases:

>100_bases
GTTTCAGATAATTCTGGCTACGGCTTGACTGTGGCGGCCGTTTTTTCCGCCCGGGCCTCGTTCTTCATCTGCTCAAACAG
ACTCACGTAGTACGGCAGGC

Product: putative para-nitrobenzyl esterase

Products: NA

Alternate protein names: Intracellular esterase B; PNB carboxy-esterase; PNBCE [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MFTQIAAEQPDLQVPTEEQIGSAYSRWRRKARSLSMATDVGFRMPSVWLAEGHSGVAPVYLYRFDYSTPLLKLLLVRAAH
ATELPYVWGNLGGSQDPALKLGDAKAAIAVSRRVRTRWINFATRGKPTGPDGEPDWPCYEEAHRACLIIGRRDAVVHDVD
AHIRATWGSKW

Sequences:

>Translated_171_residues
MFTQIAAEQPDLQVPTEEQIGSAYSRWRRKARSLSMATDVGFRMPSVWLAEGHSGVAPVYLYRFDYSTPLLKLLLVRAAH
ATELPYVWGNLGGSQDPALKLGDAKAAIAVSRRVRTRWINFATRGKPTGPDGEPDWPCYEEAHRACLIIGRRDAVVHDVD
AHIRATWGSKW
>Mature_171_residues
MFTQIAAEQPDLQVPTEEQIGSAYSRWRRKARSLSMATDVGFRMPSVWLAEGHSGVAPVYLYRFDYSTPLLKLLLVRAAH
ATELPYVWGNLGGSQDPALKLGDAKAAIAVSRRVRTRWINFATRGKPTGPDGEPDWPCYEEAHRACLIIGRRDAVVHDVD
AHIRATWGSKW

Specific function: Catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol [H]

COG id: COG2272

COG function: function code I; Carboxylesterase type B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-B carboxylesterase/lipase family [H]

Homologues:

Organism=Homo sapiens, GI68508967, Length=130, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI68508965, Length=130, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI68508957, Length=130, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002018
- InterPro:   IPR019826
- InterPro:   IPR019819
- InterPro:   IPR000997 [H]

Pfam domain/function: PF00135 COesterase [H]

EC number: NA

Molecular weight: Translated: 19145; Mature: 19145

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTQIAAEQPDLQVPTEEQIGSAYSRWRRKARSLSMATDVGFRMPSVWLAEGHSGVAPVY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEE
LYRFDYSTPLLKLLLVRAAHATELPYVWGNLGGSQDPALKLGDAKAAIAVSRRVRTRWIN
EEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FATRGKPTGPDGEPDWPCYEEAHRACLIIGRRDAVVHDVDAHIRATWGSKW
HHCCCCCCCCCCCCCCCHHHHCCCEEEEECCCCCEEEECCCEEEECCCCCC
>Mature Secondary Structure
MFTQIAAEQPDLQVPTEEQIGSAYSRWRRKARSLSMATDVGFRMPSVWLAEGHSGVAPVY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEE
LYRFDYSTPLLKLLLVRAAHATELPYVWGNLGGSQDPALKLGDAKAAIAVSRRVRTRWIN
EEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
FATRGKPTGPDGEPDWPCYEEAHRACLIIGRRDAVVHDVDAHIRATWGSKW
HHCCCCCCCCCCCCCCCHHHHCCCEEEEECCCCCEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7828905; 8969506; 9384377; 10535917 [H]