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The map label for this gene is lipU [H]

Identifier: 121637005

GI number: 121637005

Start: 1232066

End: 1232959

Strand: Direct

Name: lipU [H]

Synonym: BCG_1134

Alternate gene names: 121637005

Gene position: 1232066-1232959 (Clockwise)

Preceding gene: 121637002

Following gene: 121637006

Centisome position: 28.16

GC content: 65.21

Gene sequence:

>894_bases
GTGGCGGTCCGGCCGGTGCTAGCGGTCGGTAGCTATCTTCCGCATGCGCCCTGGCCGTGGGGTGTCATCGACCAGGCTGC
CCGGGTTCTGCTCCCAGCGTCAACGACCGTAAGGGCCGCGGTGAGCCTGCCTAATGCGTCCGCCCAACTGGTTCGGGCGT
CGGGTGTGTTGCCGGCGGACGGCACTCGACGCGCCGTCCTGTACCTGCACGGCGGCGCGTTTCTGACGTGTGGAGCAAAC
TCGCATGGACGACTCGTCGAGTTGCTCTCTAAGTTCGCTGACTCGCCTGTTCTGGTGGTCGACTATCGGTTGATTCCCAA
GCACTCGATCGGGATGGCGCTCGACGACTGTCACGACGGCTACCGGTGGCTGAGGCTGTTGGGCTATGAGCCGGAGCAGA
TCGTGCTAGCGGGCGATTCCGCGGGCGGGTATCTTGCGCTCGCTCTCGCGCAGCGGCTACAGGAAGTGGGGGAGGAGCCG
GCGGCTCTAGTCGCGATCTCGCCACTGCTGCAGCTAGCAAAGGAACACAAGCAGGCGCATCCCAACATCAAAACCGATGC
GATGTTCCCGGCAAGGGCGTTCGATGCGCTTGACGCATTGGTTGCTAGCGCAGCAGCGAGGAACCAGGTAGACGGCGAAC
CCGAAGAGCTCTATGAGCCCTTGGAGCACATCACACCGGGGCTGCCGCGGACACTGATTCACGTGTCGGGCTCCGAGGTA
TTGCTGCACGACGCTCAGTTGGCGGCGGCCAAACTGGCGGCGGCCGGGGTGCCGGCCGAGGTCCGGGTATGGCCGGGCCA
GGTCCACGACTTTCAGGTTGCGGCGTCGATGCTGCCCGAGGCGATCCGCTCGTTGCGTCAGATCGGGGAGTACATCCGCG
AGGCCACCGGGTAG

Upstream 100 bases:

>100_bases
ACTCGAGGCACGCAAGTTTGCGATCAGCGACGGCGCCCCGGTGGAGGTCGTCGAGTCTGGTCCAAGTCTTGTTGCGCGAT
TAGCTGCGCTGGCGTCACGA

Downstream 100 bases:

>100_bases
CGGGATGCCGACGGAGCGCGTGTGCCTGGCCGGCAGGCGCCTGAGACGATGAACGCATGCGGATCGCGCAACATATCAGT
GAACTCATTGGTGGTACCCC

Product: putative lipase lipU

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAVRPVLAVGSYLPHAPWPWGVIDQAARVLLPASTTVRAAVSLPNASAQLVRASGVLPADGTRRAVLYLHGGAFLTCGAN
SHGRLVELLSKFADSPVLVVDYRLIPKHSIGMALDDCHDGYRWLRLLGYEPEQIVLAGDSAGGYLALALAQRLQEVGEEP
AALVAISPLLQLAKEHKQAHPNIKTDAMFPARAFDALDALVASAAARNQVDGEPEELYEPLEHITPGLPRTLIHVSGSEV
LLHDAQLAAAKLAAAGVPAEVRVWPGQVHDFQVAASMLPEAIRSLRQIGEYIREATG

Sequences:

>Translated_297_residues
MAVRPVLAVGSYLPHAPWPWGVIDQAARVLLPASTTVRAAVSLPNASAQLVRASGVLPADGTRRAVLYLHGGAFLTCGAN
SHGRLVELLSKFADSPVLVVDYRLIPKHSIGMALDDCHDGYRWLRLLGYEPEQIVLAGDSAGGYLALALAQRLQEVGEEP
AALVAISPLLQLAKEHKQAHPNIKTDAMFPARAFDALDALVASAAARNQVDGEPEELYEPLEHITPGLPRTLIHVSGSEV
LLHDAQLAAAKLAAAGVPAEVRVWPGQVHDFQVAASMLPEAIRSLRQIGEYIREATG
>Mature_296_residues
AVRPVLAVGSYLPHAPWPWGVIDQAARVLLPASTTVRAAVSLPNASAQLVRASGVLPADGTRRAVLYLHGGAFLTCGANS
HGRLVELLSKFADSPVLVVDYRLIPKHSIGMALDDCHDGYRWLRLLGYEPEQIVLAGDSAGGYLALALAQRLQEVGEEPA
ALVAISPLLQLAKEHKQAHPNIKTDAMFPARAFDALDALVASAAARNQVDGEPEELYEPLEHITPGLPRTLIHVSGSEVL
LHDAQLAAAKLAAAGVPAEVRVWPGQVHDFQVAASMLPEAIRSLRQIGEYIREATG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=135, Percent_Identity=32.5925925925926, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=157, Percent_Identity=32.484076433121, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31682; Mature: 31551

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRPVLAVGSYLPHAPWPWGVIDQAARVLLPASTTVRAAVSLPNASAQLVRASGVLPAD
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHEECCCHHHEEEEECCCCHHHHHHHCCCCCCC
GTRRAVLYLHGGAFLTCGANSHGRLVELLSKFADSPVLVVDYRLIPKHSIGMALDDCHDG
CCCEEEEEEECCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHH
YRWLRLLGYEPEQIVLAGDSAGGYLALALAQRLQEVGEEPAALVAISPLLQLAKEHKQAH
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PNIKTDAMFPARAFDALDALVASAAARNQVDGEPEELYEPLEHITPGLPRTLIHVSGSEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEE
LLHDAQLAAAKLAAAGVPAEVRVWPGQVHDFQVAASMLPEAIRSLRQIGEYIREATG
EEEHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVRPVLAVGSYLPHAPWPWGVIDQAARVLLPASTTVRAAVSLPNASAQLVRASGVLPAD
CCCHHHHHHCCCCCCCCCCHHHHHHHHHEECCCHHHEEEEECCCCHHHHHHHCCCCCCC
GTRRAVLYLHGGAFLTCGANSHGRLVELLSKFADSPVLVVDYRLIPKHSIGMALDDCHDG
CCCEEEEEEECCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHH
YRWLRLLGYEPEQIVLAGDSAGGYLALALAQRLQEVGEEPAALVAISPLLQLAKEHKQAH
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PNIKTDAMFPARAFDALDALVASAAARNQVDGEPEELYEPLEHITPGLPRTLIHVSGSEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEE
LLHDAQLAAAKLAAAGVPAEVRVWPGQVHDFQVAASMLPEAIRSLRQIGEYIREATG
EEEHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]