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The map label for this gene is cbs

Identifier: 121637006

GI number: 121637006

Start: 1233016

End: 1234410

Strand: Direct

Name: cbs

Synonym: BCG_1135

Alternate gene names: 121637006

Gene position: 1233016-1234410 (Clockwise)

Preceding gene: 121637005

Following gene: 121637007

Centisome position: 28.19

GC content: 66.38

Gene sequence:

>1395_bases
ATGCGGATCGCGCAACATATCAGTGAACTCATTGGTGGTACCCCACTGGTTCGGCTGAACTCCGTGGTACCCGACGGCGC
CGGAACCGTGGCCGCAAAGGTCGAGTATCTCAACCCTGGCGGCAGCTCCAAGGATCGGATCGCGGTGAAGATGATCGAAG
CCGCCGAGGCCAGCGGTCAGCTGAAGCCGGGTGGCACCATCGTCGAACCCACGTCCGGCAATACCGGCGTTGGTCTGGCG
TTGGTCGCTCAGCGCCGCGGCTACAAGTGCGTGTTCGTCTGCCCGGACAAGGTCAGTGAGGATAAACGCAATGTGTTGAT
CGCCTACGGCGCCGAGGTCGTGGTGTGCCCGACGGCGGTCCCGCCGCACGATCCGGCCAGCTACTACAGTGTGTCGGACC
GGTTGGTCCGTGATATCGACGGTGCCTGGAAGCCCGACCAGTACGCCAACCCGGAGGGACCGGCAAGCCATTATGTGACC
ACCGGCCCGGAAATCTGGGCCGATACCGAGGGCAAGGTCACCCATTTCGTGGCTGGCATCGGCACCGGCGGTACCATCAC
CGGCGCTGGCCGGTACCTCAAAGAGGTGTCCGGGGGCCGAGTACGCATCGTCGGCGCCGACCCGGAGGGATCGGTCTATT
CGGGCGGTGCCGGCCGACCGTATCTGGTCGAGGGGGTCGGCGAGGATTTCTGGCCGGCGGCCTATGACCCGAGCGTGCCC
GACGAGATCATCGCGGTGTCCGACTCCGACTCGTTCGACATGACCAGGCGGCTGGCCCGCGAAGAGGCGATGTTGGTCGG
CGGGTCGTGCGGGATGGCGGTGGTTGCCGCGCTCAAGGTCGCCGAGGAAGCCGGGCCCGACGCGTTGATCGTCGTCCTGT
TGCCCGACGGCGGCCGGGGCTACATGTCGAAAATCTTCAACGACGCGTGGATGTCGTCCTATGGGTTCCTGCGCAGCCGC
CTTGACGGGTCGACCGAGCAATCCACCGTCGGTGATGTGTTGCGCCGCAAGTCCGGCGCGCTGCCCGCCCTGGTGCACAC
CCATCCGTCGGAGACCGTGCGCGACGCCATCGGGATTCTTCGCGAGTACGGGGTGTCGCAGATGCCGGTGGTCGGCGCCG
AGCCGCCGGTGATGGCCGGCGAGGTCGCCGGTAGCGTCTCGGAACGCGAGCTGCTCTCGGCCGTGTTCGAGGGCCGCGCC
AAGTTGGCCGACGCCGTGTCGGCACACATGAGCCCGCCGCTGCGGATGATAGGCGCCGGTGAATTGGTCAGTGCGGCCGG
CAAGGCGTTGCGTGATTGGGATGCGTTGATGGTGGTGGAGGAAGGCAAGCCGGTTGGGGTCATTACCCGGTACGACTTGT
TGGGCTTCTTGTCGGAGGGGGCGGGACGGCGGTAG

Upstream 100 bases:

>100_bases
CGTTGCGTCAGATCGGGGAGTACATCCGCGAGGCCACCGGGTAGCGGGATGCCGACGGAGCGCGTGTGCCTGGCCGGCAG
GCGCCTGAGACGATGAACGC

Downstream 100 bases:

>100_bases
TCGCGCAGGCAGGCGCGCCGCAATTTAGTTCGGCTACAAACAATTACGGCAGGCGGCCAGTGCCGCACAGGTCGTGGGCA
CTGACCCATTGGGCCCCGTG

Product: putative cystathionine beta-synthase CBS

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAVPPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVT
TGPEIWADTEGKVTHFVAGIGTGGTITGAGRYLKEVSGGRVRIVGADPEGSVYSGGAGRPYLVEGVGEDFWPAAYDPSVP
DEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVAEEAGPDALIVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQSTVGDVLRRKSGALPALVHTHPSETVRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAVFEGRA
KLADAVSAHMSPPLRMIGAGELVSAAGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGAGRR

Sequences:

>Translated_464_residues
MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAVPPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVT
TGPEIWADTEGKVTHFVAGIGTGGTITGAGRYLKEVSGGRVRIVGADPEGSVYSGGAGRPYLVEGVGEDFWPAAYDPSVP
DEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVAEEAGPDALIVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQSTVGDVLRRKSGALPALVHTHPSETVRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAVFEGRA
KLADAVSAHMSPPLRMIGAGELVSAAGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGAGRR
>Mature_464_residues
MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAVPPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVT
TGPEIWADTEGKVTHFVAGIGTGGTITGAGRYLKEVSGGRVRIVGADPEGSVYSGGAGRPYLVEGVGEDFWPAAYDPSVP
DEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVAEEAGPDALIVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQSTVGDVLRRKSGALPALVHTHPSETVRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAVFEGRA
KLADAVSAHMSPPLRMIGAGELVSAAGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGAGRR

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=478, Percent_Identity=39.5397489539749, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI295821200, Length=478, Percent_Identity=39.5397489539749, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI4557415, Length=478, Percent_Identity=39.5397489539749, Blast_Score=292, Evalue=6e-79,
Organism=Escherichia coli, GI2367138, Length=309, Percent_Identity=38.5113268608414, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=40.7523510971787, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=44.5482866043614, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI115535073, Length=335, Percent_Identity=41.1940298507463, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=40.3973509933775, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=42.2360248447205, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17562970, Length=314, Percent_Identity=40.1273885350318, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=285, Percent_Identity=43.1578947368421, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=332, Percent_Identity=39.4578313253012, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI32566672, Length=221, Percent_Identity=37.5565610859729, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321594, Length=505, Percent_Identity=34.4554455445545, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=34.0361445783133, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24643623, Length=476, Percent_Identity=35.0840336134454, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI20129101, Length=476, Percent_Identity=35.0840336134454, Blast_Score=244, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1077_MYCTU (Q7D8W0)

Other databases:

- EMBL:   BX842575
- EMBL:   AE000516
- PIR:   C70894
- RefSeq:   NP_335550.1
- RefSeq:   YP_177782.1
- HSSP:   Q9WZD3
- ProteinModelPortal:   Q7D8W0
- SMR:   Q7D8W0
- EnsemblBacteria:   EBMYCT00000001763
- EnsemblBacteria:   EBMYCT00000073085
- GeneID:   887108
- GeneID:   925074
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1108
- KEGG:   mtu:Rv1077
- TIGR:   MT1108
- TubercuList:   Rv1077
- GeneTree:   EBGT00050000015764
- HOGENOM:   HBG748215
- OMA:   KERKWIR
- ProtClustDB:   CLSK881170
- GO:   GO:0005737
- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00116
- TIGRFAMs:   TIGR01137

Pfam domain/function: PF00571 CBS; PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.22

Molecular weight: Translated: 48636; Mature: 48636

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS51371 CBS; PS00901 CYS_SYNTHASE; PS00217 SUGAR_TRANSPORT_2

Important sites: BINDING 74-74 BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQ
CCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEHHHHCCCC
LKPGGTIVEPTSGNTGVGLALVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAV
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCC
PPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVTTGPEIWADTEGKVTHFVAGI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCEEEEEEEC
GTGGTITGAGRYLKEVSGGRVRIVGADPEGSVYSGGAGRPYLVEGVGEDFWPAAYDPSVP
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCC
DEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVAEEAGPDALIVVLLPDGGRG
CCEEEECCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
YMSKIFNDAWMSSYGFLRSRLDGSTEQSTVGDVLRRKSGALPALVHTHPSETVRDAIGIL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
REYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAVFEGRAKLADAVSAHMSPPLRMIGAG
HHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ELVSAAGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGAGRR
HHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQ
CCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEHHHHCCCC
LKPGGTIVEPTSGNTGVGLALVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAV
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCC
PPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVTTGPEIWADTEGKVTHFVAGI
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCEEECCCCCEEEEEEEC
GTGGTITGAGRYLKEVSGGRVRIVGADPEGSVYSGGAGRPYLVEGVGEDFWPAAYDPSVP
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCC
DEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVAEEAGPDALIVVLLPDGGRG
CCEEEECCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCH
YMSKIFNDAWMSSYGFLRSRLDGSTEQSTVGDVLRRKSGALPALVHTHPSETVRDAIGIL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
REYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAVFEGRAKLADAVSAHMSPPLRMIGAG
HHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ELVSAAGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGAGRR
HHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036