BacMap

Definition	Mycobacterium bovis BCG str. Pasteur 1173P2, complete genome.
Accession	NC_008769
Length	4,374,522

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The map label for this gene is hdhA [C]

Identifier: 121636693

GI number: 121636693

Start: 894193

End: 894939

Strand: Direct

Name: hdhA [C]

Synonym: BCG_0821

Alternate gene names: 121636693

Gene position: 894193-894939 (Clockwise)

Preceding gene: 121636692

Following gene: 121636694

Centisome position: 20.44

GC content: 61.58

Gene sequence:

>747_bases
ATGTTCGACAGCAAGGTGGCTATCGTCACCGGGGCTGCCCAGGGTATCGGGCAGGCCTACGCTCAGGCGTTGGCCCGCGA
AGGTGCCTCGGTGGTCGTCGCTGACATCAACGCCGACGGTGCCGCGGCGGTAGCCAAGCAGATTGTCGCCGACGGCGGTA
CTGTGATTCATGTGCCCGTTGACGTGTCCGACGAGGATTCCGCTAAAGCCATGGTCGACCGCGCCGTCGGTGCTTTCGGC
GGCATCGACTATCTGGTGAACAATGCGGCGATCTACGGTGGCATGAAGCTCGATCTGTTGTTGACCGTGCCGTTGGACTA
CTACAAGAAATTCATGAGCGTCAACCACGACGGCGTGCTGGTGTGTACCCGCGCGGTGTACAAGCACATGGCCAAACGGG
GCGGCGGCGCGATTGTCAACCAGTCCTCGACCGCGGCCTGGCTGTATTCCAACTTCTACGGCCTGGCCAAGGTCGGTGTC
AACGGGCTGACGCAGCAGCTGGCCCGCGAGCTGGGCGGAATGAAGATAAGGATCAATGCGATCGCACCCGGACCGATCGA
CACCGAAGCTACCCGCACCGTCACCCCCGCAGAGCTGGTCAAGAACATGGTGCAGACCATCCCGCTGTCGCGGATGGGTA
CACCGGAGGATCTGGTGGGCATGTGCCTGTTCCTGCTGTCGGATTCGGCATCGTGGATCACCGGGCAGATCTTCAATGTC
GATGGCGGACAGATCATCCGGTCATGA

Upstream 100 bases:

>100_bases
ACGCGAGATGGGTCTCCTCGGCTTCGAGGAGTACTTAGAAGCCAAACTCATTGCTACCGCTGCAAATTAGCTAGCGGGTT
GACAGCGCAGAAAGGAAGCC

Downstream 100 bases:

>100_bases
CCGGCGCCGGCGCCGATGCAGAGCGGGGCGATGAGGTGGGGGCACGCCCCCACAAGTGGGAGGTACCCCCATCCGCTGGC
GGGGGAGAGCGGCGCTCATG

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MFDSKVAIVTGAAQGIGQAYAQALAREGASVVVADINADGAAAVAKQIVADGGTVIHVPVDVSDEDSAKAMVDRAVGAFG
GIDYLVNNAAIYGGMKLDLLLTVPLDYYKKFMSVNHDGVLVCTRAVYKHMAKRGGGAIVNQSSTAAWLYSNFYGLAKVGV
NGLTQQLARELGGMKIRINAIAPGPIDTEATRTVTPAELVKNMVQTIPLSRMGTPEDLVGMCLFLLSDSASWITGQIFNV
DGGQIIRS

Sequences:

>Translated_248_residues
MFDSKVAIVTGAAQGIGQAYAQALAREGASVVVADINADGAAAVAKQIVADGGTVIHVPVDVSDEDSAKAMVDRAVGAFG
GIDYLVNNAAIYGGMKLDLLLTVPLDYYKKFMSVNHDGVLVCTRAVYKHMAKRGGGAIVNQSSTAAWLYSNFYGLAKVGV
NGLTQQLARELGGMKIRINAIAPGPIDTEATRTVTPAELVKNMVQTIPLSRMGTPEDLVGMCLFLLSDSASWITGQIFNV
DGGQIIRS
>Mature_248_residues
MFDSKVAIVTGAAQGIGQAYAQALAREGASVVVADINADGAAAVAKQIVADGGTVIHVPVDVSDEDSAKAMVDRAVGAFG
GIDYLVNNAAIYGGMKLDLLLTVPLDYYKKFMSVNHDGVLVCTRAVYKHMAKRGGGAIVNQSSTAAWLYSNFYGLAKVGV
NGLTQQLARELGGMKIRINAIAPGPIDTEATRTVTPAELVKNMVQTIPLSRMGTPEDLVGMCLFLLSDSASWITGQIFNV
DGGQIIRS

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=38.1889763779528, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI19923817, Length=254, Percent_Identity=32.2834645669291, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=32.6693227091633, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI10190704, Length=249, Percent_Identity=31.7269076305221, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI126723750, Length=257, Percent_Identity=33.4630350194552, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=31.7073170731707, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7705925, Length=242, Percent_Identity=34.7107438016529, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4503301, Length=250, Percent_Identity=31.6, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=30.8641975308642, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4504505, Length=257, Percent_Identity=32.295719844358, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI4758504, Length=250, Percent_Identity=30.4, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI31542939, Length=217, Percent_Identity=31.7972350230415, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7706318, Length=244, Percent_Identity=28.2786885245902, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40254986, Length=188, Percent_Identity=32.4468085106383, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI109715829, Length=247, Percent_Identity=28.7449392712551, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI126723191, Length=190, Percent_Identity=36.8421052631579, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=29.4416243654822, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4557649, Length=207, Percent_Identity=28.5024154589372, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI20149619, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI224922801, Length=176, Percent_Identity=31.25, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4503817, Length=198, Percent_Identity=29.7979797979798, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI59889578, Length=230, Percent_Identity=25.2173913043478, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI209862901, Length=240, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI19923983, Length=240, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787905, Length=245, Percent_Identity=37.5510204081633, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=31.8367346938775, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1790717, Length=246, Percent_Identity=30.0813008130081, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=32.8063241106719, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=33.0677290836653, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=31.0204081632653, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082160, Length=247, Percent_Identity=31.1740890688259, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=34.251968503937, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=30.3846153846154, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=28.2868525896414, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=28.0155642023346, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367365, Length=248, Percent_Identity=26.6129032258064, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787526, Length=253, Percent_Identity=24.1106719367589, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787820, Length=182, Percent_Identity=27.4725274725275, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787545, Length=247, Percent_Identity=26.7206477732794, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=36, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=33.8709677419355, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=34.136546184739, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=31.4741035856574, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17562910, Length=259, Percent_Identity=29.7297297297297, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=249, Percent_Identity=31.3253012048193, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=29.0076335877863, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=31.8897637795276, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=259, Percent_Identity=31.2741312741313, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17560332, Length=260, Percent_Identity=32.6923076923077, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=32.183908045977, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531453, Length=259, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562908, Length=262, Percent_Identity=30.1526717557252, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=30.4511278195489, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=31.5789473684211, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=29.6153846153846, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=29.1187739463602, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=250, Percent_Identity=30.4, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=28.4090909090909, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=31.4741035856574, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=253, Percent_Identity=28.8537549407115, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=27.8625954198473, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=261, Percent_Identity=30.2681992337165, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=27.3764258555133, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=28.1368821292776, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=253, Percent_Identity=31.2252964426877, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17568967, Length=257, Percent_Identity=30.7392996108949, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538486, Length=260, Percent_Identity=30.3846153846154, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=266, Percent_Identity=29.3233082706767, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982365, Length=194, Percent_Identity=29.8969072164948, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=201, Percent_Identity=29.8507462686567, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=170, Percent_Identity=28.2352941176471, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17558628, Length=195, Percent_Identity=31.2820512820513, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=251, Percent_Identity=26.6932270916335, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=191, Percent_Identity=29.8429319371728, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=191, Percent_Identity=29.8429319371728, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=30.2788844621514, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322861, Length=255, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=33.596837944664, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24639444, Length=245, Percent_Identity=35.1020408163265, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=30.078125, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=31.8897637795276, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17737361, Length=255, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=31.7460317460317, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI116007236, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007234, Length=202, Percent_Identity=34.6534653465347, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=29.5275590551181, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=28.8537549407115, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357673, Length=247, Percent_Identity=27.5303643724696, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007232, Length=189, Percent_Identity=33.8624338624339, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364494, Length=226, Percent_Identity=29.646017699115, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=29.718875502008, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI116007238, Length=185, Percent_Identity=27.5675675675676, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641232, Length=202, Percent_Identity=28.2178217821782, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 25994; Mature: 25994

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDSKVAIVTGAAQGIGQAYAQALAREGASVVVADINADGAAAVAKQIVADGGTVIHVPV
CCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEE
DVSDEDSAKAMVDRAVGAFGGIDYLVNNAAIYGGMKLDLLLTVPLDYYKKFMSVNHDGVL
CCCCCHHHHHHHHHHHHHHCCHHHHHCCEEEECCEEEEEEEECCHHHHHHHHCCCCCCEE
VCTRAVYKHMAKRGGGAIVNQSSTAAWLYSNFYGLAKVGVNGLTQQLARELGGMKIRINA
EHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
IAPGPIDTEATRTVTPAELVKNMVQTIPLSRMGTPEDLVGMCLFLLSDSASWITGQIFNV
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
DGGQIIRS
CCCCEECC
>Mature Secondary Structure
MFDSKVAIVTGAAQGIGQAYAQALAREGASVVVADINADGAAAVAKQIVADGGTVIHVPV
CCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEE
DVSDEDSAKAMVDRAVGAFGGIDYLVNNAAIYGGMKLDLLLTVPLDYYKKFMSVNHDGVL
CCCCCHHHHHHHHHHHHHHCCHHHHHCCEEEECCEEEEEEEECCHHHHHHHHCCCCCCEE
VCTRAVYKHMAKRGGGAIVNQSSTAAWLYSNFYGLAKVGVNGLTQQLARELGGMKIRINA
EHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
IAPGPIDTEATRTVTPAELVKNMVQTIPLSRMGTPEDLVGMCLFLLSDSASWITGQIFNV
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
DGGQIIRS
CCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]