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The map label for this gene is garR [C]

Identifier: 121636694

GI number: 121636694

Start: 895037

End: 895924

Strand: Direct

Name: garR [C]

Synonym: BCG_0822

Alternate gene names: 121636694

Gene position: 895037-895924 (Clockwise)

Preceding gene: 121636693

Following gene: 121636695

Centisome position: 20.46

GC content: 65.99

Gene sequence:

>888_bases
ATGACCGCTCACCCGGAGACACCACGCCTGGGATATATCGGCTTGGGTAATCAAGGCGCGCCGATGGCTAAGCGTCTGCT
CGATTGGCCTGGCGGACTGACCGTTTTCGATGTGCGGGTCGAGGCCATGGCACCGTTCGTCGAGGGCGGCGCCACCGCAG
CGGCAAGCGTCTCCGACGTCGCCGAAGCCGACATCATCAGCATCACCGTGTTCGACGACGCGCAGGTGAGTTCGGTGATC
ACCGCCGACAACGGACTGGCGACGCACGCCAAGCCCGGCACTATTGTCGCGATTCACTCCACCATCGCCGACACGACAGC
AGTCGATCTGGCCGAAAAGCTCAAGCCGCAGGGGATCCACATCGTGGATGCACCGGTCAGCGGCGGCGCGGCGGCGGCCG
CCAAGGGTGAGTTGGCCGTGATGGTCGGCGCTGACGACGAGGCGTTCCAGCGGATTAAAGAGCCATTTTCGAGGTGGGCT
TCGCTGTTGATTCATGCCGGGGAACCGGGCGCTGGCACCCGGATGAAACTGGCGCGCAACATGTTGACTTTCGTCTCTTA
TGCCGCCGCCGCCGAGGCGCAGCGGCTGGCCGAAGCCTGTGGCTTAGACCTCGTGGCGCTCGGGAAGGTGGTGCGGCACA
GCGACTCATTCACCGGCGGCGCGGGAGCGATCATGTTCCGCAACACCACTGCGCCGATGGAGCCGGCTGACCCGCTGCGG
CCGTTGTTGGAGCACACCCGCGGCCTGGGTGAGAAAGACCTGAGTCTGGCGTTGGCCCTGGGCGAGGTGGTATCGGTCGA
CCTGCCGCTGGCCCAGCTGGCGCTGCAACGGCTGGCCGCCGGCCTCGGGGTACCGCACCCGGACACCGAGCCAGCAAAGG
AGACATGA

Upstream 100 bases:

>100_bases
TGACCGGCGCCGGCGCCGATGCAGAGCGGGGCGATGAGGTGGGGGCACGCCCCCACAAGTGGGAGGTACCCCCATCCGCT
GGCGGGGGAGAGCGGCGCTC

Downstream 100 bases:

>100_bases
TGGACGAGCTGCGCCGCACCGGCCTGGACAAAATGAACGAGGTTTACGCCTGGGACATGCCCGACATGCCAGGTGAGTTT
TTTGCCCTGACCGTCGATCA

Product: putative dehydrogenase/reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTAHPETPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVI
TADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWA
SLLIHAGEPGAGTRMKLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLR
PLLEHTRGLGEKDLSLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET

Sequences:

>Translated_295_residues
MTAHPETPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVI
TADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWA
SLLIHAGEPGAGTRMKLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLR
PLLEHTRGLGEKDLSLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
>Mature_294_residues
TAHPETPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVIT
ADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWAS
LLIHAGEPGAGTRMKLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRP
LLEHTRGLGEKDLSLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=210, Percent_Identity=34.7619047619048, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI40556376, Length=270, Percent_Identity=25.1851851851852, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=33.9285714285714, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790315, Length=262, Percent_Identity=31.2977099236641, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786719, Length=262, Percent_Identity=29.7709923664122, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789092, Length=278, Percent_Identity=33.4532374100719, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=31.1355311355311, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24655230, Length=276, Percent_Identity=28.9855072463768, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922568, Length=276, Percent_Identity=28.9855072463768, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574115, Length=274, Percent_Identity=23.3576642335766, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y770_MYCTU (P71825)

Other databases:

- EMBL:   BX842574
- EMBL:   AE000516
- PIR:   E70707
- RefSeq:   NP_215284.1
- RefSeq:   NP_335222.1
- ProteinModelPortal:   P71825
- SMR:   P71825
- EnsemblBacteria:   EBMYCT00000000782
- EnsemblBacteria:   EBMYCT00000069839
- GeneID:   888868
- GeneID:   926097
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0794
- KEGG:   mtu:Rv0770
- TIGR:   MT0794
- TubercuList:   Rv0770
- GeneTree:   EBGT00050000015861
- HOGENOM:   HBG729179
- OMA:   VVRHTDA
- ProtClustDB:   CLSK790725
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.1.31

Molecular weight: Translated: 30446; Mature: 30315

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 176-176 BINDING 101-101 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHPETPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDV
CCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHH
AEADIISITVFDDAQVSSVITADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIH
CCCCEEEEEEECCCCHHEEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHCCCCCEE
IVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTRMKLARN
EEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
MLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHH
PLLEHTRGLGEKDLSLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAHPETPRLGYIGLGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDV
CCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHH
AEADIISITVFDDAQVSSVITADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIH
CCCCEEEEEEECCCCHHEEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHCCCCCEE
IVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTRMKLARN
EEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
MLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHH
PLLEHTRGLGEKDLSLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036