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The map label for this gene is pks6a [H]

Identifier: 121636318

GI number: 121636318

Start: 516545

End: 517927

Strand: Direct

Name: pks6a [H]

Synonym: BCG_0443

Alternate gene names: 121636318

Gene position: 516545-517927 (Clockwise)

Preceding gene: 121636317

Following gene: 121636319

Centisome position: 11.81

GC content: 52.86

Gene sequence:

>1383_bases
ATGACAGACGGTTCGGTCACTGCGGATAAGCTTCAAAAATGGTTTCGAGAGTACTTGTCCACGCATATCGAGTGTCATCC
AAATGAGGTCAGCCTAGACGTTCCGATTAGAGATTTAGGTTTGAAATCGATTGATGTCTTAGCGATTCCCGGCGACCTCG
GTGACAGATTTGGGTTTTGTATTCCCGATTTGGCCGTTTGGGATAATCCTAGCGCTAATGATTTGATTGATAGTCTGTTG
AACCAGCGTAGTGCTGACTCGTTAAGAGAGAGTCATGGACACGCCGACAGGAACACGCAGGGTCGGGGCAGCATAAACGA
GCCGGTTGCGGTCATCGGAGTGGGCTGTCGATTTCCGGGAGATATTGACGGCCCGGAACGGCTATGGGACTTTCTGACCG
AGAAGAAGTGTGCGATAACAGCGTATCCAGATCGTGGGTTCACGAATGCTGGAACTTTCGCGGAGTCCGGAGGCTTTTTA
AAGGATGTCGCGGGTTTCGATAATAGATTTTTTGATATCCCGCCGGACGAGGCTCTGCGAATGGATCCGCAACAACGGTT
GTTACTGGAGGTCTCTTGGGAAGCGTTAGAGCATGCAGGAATTATTCCTGAGTCATTAAGACTTTCACGTACGGGCGTAT
TCGTTGGGGTGTCGTCAACTGACTACGTCCGGCTTGTGTCAGCTAGCGCTCAGCAAAAGTCTACTATTTGGGATAACACC
GGCGGTTCTTCGAGTATTATTGCCAATAGAATCTCATACTTTCTCGATATTCAGGGTCCGTCCATTGTCATTGACACGGC
ATGCTCGTCATCCCTGGTCGCCGTGCATCTAGCCTGTCGAAGTCTCAGTACCTGGGACTGCGATATCGCACTTGTCGGTG
GGACGAATGTTCTTATTTCACCAGAACCATGGGGTGGGTTTAGGGAAGCGGGCATCTTGTCGCAGACAGGCTGCTGTCAC
GCGTTCGATAAATCCGCCGACGGGATGGTACGCGGTGAGGGATGCGGAGTTATCGTGCTGCAGCGCCTCAGTGATGCACG
CCTTGAGGGCCGGCGGATATTAGCGATTCTGACGGGTTCAGCGGTCAATCAGGACGGTAAGTCCAACGGTATTATGGCGC
CAAATCCTAGTGCGCAAATTGGTGTTCTTGAAAATGCATGCAAGAGCGCTCGCGTCGATCCGCTGGAAATCGGCTACGTC
GAGGCCCACGGGACCGGAACGTCGTTAGGGGATAGGATCGAGGCGCACGCCTTAGGCATGGTCTTTGGTCGCAAGAGACC
GGGATCTGGGCCCCTGATGATCGGGAGCATCAAGCCGAATATCGGCCATCTGGAAGGTGCGGCTGGCATCGCCGGATTGA
TCAAGGCGGGTGTTGATGGTTGA

Upstream 100 bases:

>100_bases
CGCCCGGGGCGCTACCGAAGACCACCAGCGGAAAGATTAGCCGGGCGGCATGCGCAAAGCAGTACGGAGCAAATAAGTTG
CAACGAGTAGCAACGTTCCC

Downstream 100 bases:

>100_bases
GCGTGGCTCGCTGCTTCCGAGCGGGGGGTTTACGGAGCCAAATCCAGCTATCCCATTCACGGAATTGGGCCTGAGAGTTG
TAGACGAACTTCAGGAGTGG

Product: putative membrane bound polyketide synthase pks6a

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFCIPDLAVWDNPSANDLIDSLL
NQRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPGDIDGPERLWDFLTEKKCAITAYPDRGFTNAGTFAESGGFL
KDVAGFDNRFFDIPPDEALRMDPQQRLLLEVSWEALEHAGIIPESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNT
GGSSSIIANRISYFLDIQGPSIVIDTACSSSLVAVHLACRSLSTWDCDIALVGGTNVLISPEPWGGFREAGILSQTGCCH
AFDKSADGMVRGEGCGVIVLQRLSDARLEGRRILAILTGSAVNQDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYV
EAHGTGTSLGDRIEAHALGMVFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAGVDG

Sequences:

>Translated_460_residues
MTDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFCIPDLAVWDNPSANDLIDSLL
NQRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPGDIDGPERLWDFLTEKKCAITAYPDRGFTNAGTFAESGGFL
KDVAGFDNRFFDIPPDEALRMDPQQRLLLEVSWEALEHAGIIPESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNT
GGSSSIIANRISYFLDIQGPSIVIDTACSSSLVAVHLACRSLSTWDCDIALVGGTNVLISPEPWGGFREAGILSQTGCCH
AFDKSADGMVRGEGCGVIVLQRLSDARLEGRRILAILTGSAVNQDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYV
EAHGTGTSLGDRIEAHALGMVFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAGVDG
>Mature_459_residues
TDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFCIPDLAVWDNPSANDLIDSLLN
QRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPGDIDGPERLWDFLTEKKCAITAYPDRGFTNAGTFAESGGFLK
DVAGFDNRFFDIPPDEALRMDPQQRLLLEVSWEALEHAGIIPESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNTG
GSSSIIANRISYFLDIQGPSIVIDTACSSSLVAVHLACRSLSTWDCDIALVGGTNVLISPEPWGGFREAGILSQTGCCHA
FDKSADGMVRGEGCGVIVLQRLSDARLEGRRILAILTGSAVNQDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYVE
AHGTGTSLGDRIEAHALGMVFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAGVDG

Specific function: Polyketide synthase involved in phenolpthiocerol synthesis [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=350, Percent_Identity=37.7142857142857, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI8923559, Length=211, Percent_Identity=34.1232227488152, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1787337, Length=210, Percent_Identity=32.8571428571429, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI212642053, Length=350, Percent_Identity=40.2857142857143, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17550940, Length=433, Percent_Identity=30.715935334873, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71983541, Length=280, Percent_Identity=30, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983535, Length=189, Percent_Identity=35.978835978836, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320904, Length=368, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581345, Length=295, Percent_Identity=40.6779661016949, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI221330659, Length=395, Percent_Identity=34.9367088607595, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI19920632, Length=395, Percent_Identity=34.9367088607595, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24644341, Length=283, Percent_Identity=30.7420494699647, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR000794
- InterPro:   IPR002198
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020801
- InterPro:   IPR020841
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF00106 adh_short; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 48982; Mature: 48851

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50075 ACP_DOMAIN ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFC
CCCCCCCHHHHHHHHHHHHHHCEEECCCEEEEECCHHHCCCCEEEEEEECCCCCCCCCCC
IPDLAVWDNPSANDLIDSLLNQRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPG
CCCCEECCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
DIDGPERLWDFLTEKKCAITAYPDRGFTNAGTFAESGGFLKDVAGFDNRFFDIPPDEALR
CCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCHHHC
MDPQQRLLLEVSWEALEHAGIIPESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNT
CCHHHHEEEEEEHHHHHHCCCCCHHHEEEECEEEEEECCCCEEEEEECCCHHCCCCCCCC
GGSSSIIANRISYFLDIQGPSIVIDTACSSSLVAVHLACRSLSTWDCDIALVGGTNVLIS
CCCHHHHHHEEEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEC
PEPWGGFREAGILSQTGCCHAFDKSADGMVRGEGCGVIVLQRLSDARLEGRRILAILTGS
CCCCCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEHHHCCCCCCCEEEEEEECC
AVNQDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYVEAHGTGTSLGDRIEAHALGM
CCCCCCCCCCEECCCCCCEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
VFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAGVDG
HHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFC
CCCCCCHHHHHHHHHHHHHHCEEECCCEEEEECCHHHCCCCEEEEEEECCCCCCCCCCC
IPDLAVWDNPSANDLIDSLLNQRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPG
CCCCEECCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCC
DIDGPERLWDFLTEKKCAITAYPDRGFTNAGTFAESGGFLKDVAGFDNRFFDIPPDEALR
CCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCCCCHHHC
MDPQQRLLLEVSWEALEHAGIIPESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNT
CCHHHHEEEEEEHHHHHHCCCCCHHHEEEECEEEEEECCCCEEEEEECCCHHCCCCCCCC
GGSSSIIANRISYFLDIQGPSIVIDTACSSSLVAVHLACRSLSTWDCDIALVGGTNVLIS
CCCHHHHHHEEEEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEC
PEPWGGFREAGILSQTGCCHAFDKSADGMVRGEGCGVIVLQRLSDARLEGRRILAILTGS
CCCCCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEHHHCCCCCCCEEEEEEECC
AVNQDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYVEAHGTGTSLGDRIEAHALGM
CCCCCCCCCCEECCCCCCEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
VFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAGVDG
HHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]