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The map label for this gene is fadD30 [H]

Identifier: 121636317

GI number: 121636317

Start: 514791

End: 516548

Strand: Direct

Name: fadD30 [H]

Synonym: BCG_0442

Alternate gene names: 121636317

Gene position: 514791-516548 (Clockwise)

Preceding gene: 121636313

Following gene: 121636318

Centisome position: 11.77

GC content: 53.87

Gene sequence:

>1758_bases
ATGTCTGTTATATCCACGTTGCGCGACCGTGCGACCACGACTCCAAGCGACGAAGCCTTTGTGTTCATGGATTACGACAC
AAAAACCGGCGACCAAATTGACCGAATGACGTGGAGTCAATTATATTCTCGCGTCACCGCCGTGTCTGCGTATCTAATAA
GTTATGGCCGGCATGCTGACCGACGAAGGACCGCAGCGATATCAGCTCCGCAAGGTCTGGACTATGTTGCAGGATTTCTA
GGAGCACTGTGCGCCGGATGGGCGCCGGTTCCGTTACCAGAACCGCTGGGCAGCCTACGCGATAAGCGGACTGGACTGGC
TGTACTCGACTGTGCCGCCGACGTCGTGCTGACGACGTCGCAAGCCGAAACGCGGGTCAGGGCCACGATAGCTACACATG
GGGCGTCTGTAACTACGCCGGTCATAGCGTTGGATACATTGGACGAGCCATCCGGAGATAACTGTGATCTCGATTCTCAA
CTATCAGACTGGAGTTCGTATTTGCAGTATACTTCGGGTTCAACGGCCAACCCCCGTGGTGTGGTTTTATCCATGCGTAA
CGTTACGGAAAATGTCGACCAAATTATCCGTAACTATTTTCGCCATGAGGGCGGCGCGCCGAGGTTGCCCAGCTCGGTCG
TTTCGTGGTTGCCGCTTTACCATGACATGGGTTTAATGGTTGGCCTCTTTATTCCGTTGTTTGTCGGATGTCCGGTTATC
CTGACGAGCCCAGAGGCATTTATCCGTAAGCCTGCCAGATGGATGCAACTGCTTGCTAAACACCAGGCGCCATTTTCGGC
CGCGCCGAACTTCGCATTCGATTTGGCCGTCGCTAAAACTTCCGAAGAGGACATGGCGGGGCTGGATTTAGGCCACGTAA
ATACAATAATCAACGGCTCGGAGCAGGTACAGCCAAATACAATAACCAAATTCCTCCGCCGGTTCCGTCCCTACAATTTG
ATGCCCGCAGCGGTCAAGCCATCATACGGGATGGCTGAAGCGGTGGTTTACCTGGCGACGACGAAGGCGGGATCACCTCC
AACGTCAACCGAGTTCGATGCTGATAGCTTGGCTCGAGGCCACGCGGAGCTAAGTACTTTCGAAACTGAGCGTGCAACGC
GTTTAATACGCTACCACAGCGACGACAAGGAACCGTTGCTTCGGATTGTCGATCCGGACTCGAATATCGAGCTCGGACCG
GGACGTATCGGCGAGATTTGGATTCACGGTAAGAATGTGTCTACCGGATATCACAATGCAGACGACGCGCTCAATCGAGA
TAAGTTCCAGGCCAGCATCCGGGAGGCCTCTGCGGGAACGCCAAGGTCGCCGTGGCTTCGCACGGGAGACTTGGGATTCA
TAGTAGGAGATGAGTTCTACATCGTCGGCCGTATGAAAGATCTCATTATCCAAGACGGTGTAAACCATTATCCCGATGAT
ATCGAAACTACGGTCAAGGAGTTTACCGGTGGCCGGGTCGCGGCATTTTCAGTATCCGACGACGGGGTGGAGCATTTGGT
CATTGCGGCCGAGGTAAGGACTGAGCATGGGCCCGATAAAGTGACTATTATGGATTTCTCGACGATCAAAAGGCTGGTCG
TATCGGCGTTGTCGAAATTACATGGCCTGCATGTAACAGATTTTCTTCTGGTACCGCCCGGGGCGCTACCGAAGACCACC
AGCGGAAAGATTAGCCGGGCGGCATGCGCAAAGCAGTACGGAGCAAATAAGTTGCAACGAGTAGCAACGTTCCCATGA

Upstream 100 bases:

>100_bases
AGGAGTGGAAACATGAGCGGCCCGACGGGAAATTCGATGCCCAGACAGCTCGGCGGCCGGGTGGCCAGGATCGTTACCGG
GTAAGGGATCGCCACTCCCA

Downstream 100 bases:

>100_bases
CAGACGGTTCGGTCACTGCGGATAAGCTTCAAAAATGGTTTCGAGAGTACTTGTCCACGCATATCGAGTGTCATCCAAAT
GAGGTCAGCCTAGACGTTCC

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFL
GALCAGWAPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQ
LSDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGSEQVQPNTITKFLRRFRPYNL
MPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGP
GRIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTT
SGKISRAACAKQYGANKLQRVATFP

Sequences:

>Translated_585_residues
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFL
GALCAGWAPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQ
LSDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGSEQVQPNTITKFLRRFRPYNL
MPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGP
GRIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTT
SGKISRAACAKQYGANKLQRVATFP
>Mature_584_residues
SVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFLG
ALCAGWAPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQL
SDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVIL
TSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGSEQVQPNTITKFLRRFRPYNLM
PAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPG
RIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDDI
ETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTTS
GKISRAACAKQYGANKLQRVATFP

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=504, Percent_Identity=24.8015873015873, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI225735629, Length=536, Percent_Identity=24.6268656716418, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI45827692, Length=536, Percent_Identity=24.6268656716418, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI55749758, Length=599, Percent_Identity=24.2070116861436, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI225735625, Length=612, Percent_Identity=22.7124183006536, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI45827694, Length=581, Percent_Identity=21.8588640275387, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI156151445, Length=421, Percent_Identity=23.5154394299287, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=577, Percent_Identity=25.1299826689775, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193202679, Length=628, Percent_Identity=21.9745222929936, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=550, Percent_Identity=23.4545454545455, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=22.3175965665236, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63830; Mature: 63699

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHAD
CCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRRTAAISAPQGLDYVAGFLGALCAGWAPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTS
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEECC
QAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQLSDWSSYLQYTSGSTANPRG
CHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
VVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGS
EECCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHCCCCCCHHHHHCCCC
EQVQPNTITKFLRRFRPYNLMPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCHHHHHHC
HAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPGRIGEIWIHGKNVSTGYHNA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCC
DDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
HHHCCHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCEEEEEHHHHHHHHCCCCCCCCH
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKL
HHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
HGLHVTDFLLVPPGALPKTTSGKISRAACAKQYGANKLQRVATFP
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure 
SVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHAD
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRRTAAISAPQGLDYVAGFLGALCAGWAPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTS
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEECC
QAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQLSDWSSYLQYTSGSTANPRG
CHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
VVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGS
EECCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHCCCCCCHHHHHCCCC
EQVQPNTITKFLRRFRPYNLMPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCHHHHHHC
HAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPGRIGEIWIHGKNVSTGYHNA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCC
DDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
HHHCCHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCEEEEEHHHHHHHHCCCCCCCCH
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKL
HHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
HGLHVTDFLLVPPGALPKTTSGKISRAACAKQYGANKLQRVATFP
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]