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The map label for this gene is gcvA [H]

Identifier: 120613355

GI number: 120613355

Start: 5271071

End: 5271607

Strand: Direct

Name: gcvA [H]

Synonym: Aave_4727

Alternate gene names: 120613355

Gene position: 5271071-5271607 (Clockwise)

Preceding gene: 120613349

Following gene: 120613356

Centisome position: 98.47

GC content: 73.18

Gene sequence:

>537_bases
TTGCGCCTGGCGCCGGACGTCGATGTGCACGTGAGCACCAGCGACCGCGTGGTCGATCTGGCATCCGAAGACATCCATTT
CGCGGTACGCCATGGCCTGGGCGTGGCACCCGGGCTGCTGGCCGAGGTGCTGGTGGCCGACGACCTGGTGCCGGTCTGCA
GCCCGCGCCTGATCGCGCCCCGGCGCGTGCCCCGCGTCACCGACATCACCGGCGCCCGGCTGCTGCACGACGGGCACCGT
GGCGACTGGCGGCTCTGGTGCGAGGCGAACGGGGCCTTGGGCGTGGACTGCAACCAGGGCGTGGTGTTCACGCATTCCAA
CGGCTCGATCGAGGCTGCCCTGGCAGGCCGGGGTTTCGCCCTGGCGCGGCAGGGCTTCGTGGCGCAGGAGATCGCGGCGC
GCGCGCTCGTGGCCGTGCGGTCGGCCGCGCTGGCGACGCCGCTGGCCTACCGGCTGGTGCACCGGCCCGAAGTGCTGGTG
GACCCGGCGCTGCGCGGCTTTCGCGACTGGATCACCGCACAGGCCACCGCCTCCTGA

Upstream 100 bases:

>100_bases
GGAAGCCCGCGAGGCCGTAGCAGGCCGTGGCCGCCAGGCACGCCGCGATGCCCGCCGCGACCGGCATGGTGAGGGCGACC
GGCCCGGCCTGGGGGATTTC

Downstream 100 bases:

>100_bases
GCCGGGCGCACCGGCATGCCGGCCCGCGGCCCGCCGCCACCGGCTTCGTGCCTGCGGGCACGTATTCGCGCGCATGCTTA
CAGAAGATCTCGCGCCCTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MRLAPDVDVHVSTSDRVVDLASEDIHFAVRHGLGVAPGLLAEVLVADDLVPVCSPRLIAPRRVPRVTDITGARLLHDGHR
GDWRLWCEANGALGVDCNQGVVFTHSNGSIEAALAGRGFALARQGFVAQEIAARALVAVRSAALATPLAYRLVHRPEVLV
DPALRGFRDWITAQATAS

Sequences:

>Translated_178_residues
MRLAPDVDVHVSTSDRVVDLASEDIHFAVRHGLGVAPGLLAEVLVADDLVPVCSPRLIAPRRVPRVTDITGARLLHDGHR
GDWRLWCEANGALGVDCNQGVVFTHSNGSIEAALAGRGFALARQGFVAQEIAARALVAVRSAALATPLAYRLVHRPEVLV
DPALRGFRDWITAQATAS
>Mature_178_residues
MRLAPDVDVHVSTSDRVVDLASEDIHFAVRHGLGVAPGLLAEVLVADDLVPVCSPRLIAPRRVPRVTDITGARLLHDGHR
GDWRLWCEANGALGVDCNQGVVFTHSNGSIEAALAGRGFALARQGFVAQEIAARALVAVRSAALATPLAYRLVHRPEVLV
DPALRGFRDWITAQATAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=177, Percent_Identity=35.0282485875706, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 19004; Mature: 19004

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAPDVDVHVSTSDRVVDLASEDIHFAVRHGLGVAPGLLAEVLVADDLVPVCSPRLIAP
CCCCCCCEEEECCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEECC
RRVPRVTDITGARLLHDGHRGDWRLWCEANGALGVDCNQGVVFTHSNGSIEAALAGRGFA
CCCCCCCCCCCHHEEECCCCCCEEEEEECCCEEECCCCCCEEEEECCCCEEEEECCCCEE
LARQGFVAQEIAARALVAVRSAALATPLAYRLVHRPEVLVDPALRGFRDWITAQATAS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLAPDVDVHVSTSDRVVDLASEDIHFAVRHGLGVAPGLLAEVLVADDLVPVCSPRLIAP
CCCCCCCEEEECCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEECC
RRVPRVTDITGARLLHDGHRGDWRLWCEANGALGVDCNQGVVFTHSNGSIEAALAGRGFA
CCCCCCCCCCCHHEEECCCCCCEEEEEECCCEEECCCCCCEEEEECCCCEEEEECCCCEE
LARQGFVAQEIAARALVAVRSAALATPLAYRLVHRPEVLVDPALRGFRDWITAQATAS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]