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The map label for this gene is purB [H]

Identifier: 120613300

GI number: 120613300

Start: 5214186

End: 5215565

Strand: Direct

Name: purB [H]

Synonym: Aave_4669

Alternate gene names: 120613300

Gene position: 5214186-5215565 (Clockwise)

Preceding gene: 120613298

Following gene: 120613301

Centisome position: 97.41

GC content: 66.67

Gene sequence:

>1380_bases
ATGAGCCTGTCCACCATCAACGCCCTCTCTCCGCTCGACGGCCGCTACGCCGCCAAACTGTCCGCCCTGCGCCCCATCAT
GAGCGAGCACGGCTACATGCACCGCCGCGTGCAGGTGGAGGTGGCGTGGTTCATCGCGCTGTCGGACGCAGGCTTCGAGC
AGTTCAAGCCCCTGACCACCGGCGCGCGCGCCTACCTGCTGAGCCTGGTGAAGAATTTCTCCGAGGCCGACTCGCAGGCC
ATCAAGGACATCGAGAAGACCACCAACCACGACGTGAAGGCGGTCGAATACTGGATCAAGTCCAAGTTCGAGGCCCGCCC
GGAGCTCGAGCGCGCCGCCGAGTTCGTGCATTTCGCCTGCACCAGCGAAGACATCAACAACACCAGCCACGCGCTGCAGC
TGCGCTCCGGACGCGACCAGGTCATCCTGCCCGGCCTGGACCGCATCGTCCTGAAACTGCGCGAAATGGCGCACGCCTAC
GCCGAAGTCCCCATGCTGAGCCGCACCCACGGCCAGACCGCCAGCCCCACCACCGTGGGCAAGGAACTGGCCAACGTCGT
CGTGCGCCTGCAGGCCGCGTGCGACCGCATCGCCGGCGTGAAGATGCTGGGCAAGATGAACGGCGCCGTGGGCAACTACA
ACGCCCACCTCTCCGCCTGGCCCGACTTCGACTGGGAAGCCTTCAGCAGGAAGGTCGTCGAGACGCCCGAGCCGCTGGGC
CTGGGCCTCACCTTCCAGCCCTATTCGATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGACGCCATCGCCCG
CGCCAACACCATCCTGGTCGATCTCTCGCGCGACATCTGGGGCTATGTGTCGCTGGGCTACTTCAAGCAGCGCCTGAAGG
CCGGCGAGATCGGCTCGTCCACCATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACGCCGAAGGCAACCTGGGCCTG
GCCAACGCACTGCTGCGCCACCTGTCCGAGAAACTGCCCGTCAGCCGCTGGCAGCGCGACCTCACCGACAGCACGGTGCT
GCGCAACATCGGCGTGGCGATGGGCTACGCCGCGCTGGCCTATACCTCGCTCATGACCGGCCTGAACAAGCTGGAACTGA
ACGAAGAGGCCCTCGCAGCCGACCTGGACGCCAGTTGGGAAGTGCTGGCCGAGCCGATCCAGACCGTCATGCGCCGCTAC
GGCGTGCAGGGCGCCTACGAGAAGCTCAAGGAAGTCACGCGCGGCAAGACCGTGACCGCCGAGGCGCTGCACGCGCTGAT
CGCCGGCCTGGAAATCCCCCAGGCCGACAAGGACCGCCTGCTGGCCATGACGCCGGGCAGCTACGTCGGCAAGGCCGCCG
AGCTGGCGCGCCGGGTCTGA

Upstream 100 bases:

>100_bases
GGGCGGCCGCCGATTCTAGGGGGCCGGGCGCACTCGCGCTGCATCCGGCGGGACCCGGGTGCGGCGCCCGCGCCACGGTC
GGCACCTACAATCCCGGCCC

Downstream 100 bases:

>100_bases
CCATGGCCATCAAGTCCACCATCTTCAAGGCGCACCTCGCGATCGCCGACATCGACCACGGCTACTACGCCGACCACGCC
CTGACCCTGGCGCGCCACCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSLSTINALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWFIALSDAGFEQFKPLTTGARAYLLSLVKNFSEADSQA
IKDIEKTTNHDVKAVEYWIKSKFEARPELERAAEFVHFACTSEDINNTSHALQLRSGRDQVILPGLDRIVLKLREMAHAY
AEVPMLSRTHGQTASPTTVGKELANVVVRLQAACDRIAGVKMLGKMNGAVGNYNAHLSAWPDFDWEAFSRKVVETPEPLG
LGLTFQPYSIQIEPHDYMAELFDAIARANTILVDLSRDIWGYVSLGYFKQRLKAGEIGSSTMPHKVNPIDFENAEGNLGL
ANALLRHLSEKLPVSRWQRDLTDSTVLRNIGVAMGYAALAYTSLMTGLNKLELNEEALAADLDASWEVLAEPIQTVMRRY
GVQGAYEKLKEVTRGKTVTAEALHALIAGLEIPQADKDRLLAMTPGSYVGKAAELARRV

Sequences:

>Translated_459_residues
MSLSTINALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWFIALSDAGFEQFKPLTTGARAYLLSLVKNFSEADSQA
IKDIEKTTNHDVKAVEYWIKSKFEARPELERAAEFVHFACTSEDINNTSHALQLRSGRDQVILPGLDRIVLKLREMAHAY
AEVPMLSRTHGQTASPTTVGKELANVVVRLQAACDRIAGVKMLGKMNGAVGNYNAHLSAWPDFDWEAFSRKVVETPEPLG
LGLTFQPYSIQIEPHDYMAELFDAIARANTILVDLSRDIWGYVSLGYFKQRLKAGEIGSSTMPHKVNPIDFENAEGNLGL
ANALLRHLSEKLPVSRWQRDLTDSTVLRNIGVAMGYAALAYTSLMTGLNKLELNEEALAADLDASWEVLAEPIQTVMRRY
GVQGAYEKLKEVTRGKTVTAEALHALIAGLEIPQADKDRLLAMTPGSYVGKAAELARRV
>Mature_458_residues
SLSTINALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWFIALSDAGFEQFKPLTTGARAYLLSLVKNFSEADSQAI
KDIEKTTNHDVKAVEYWIKSKFEARPELERAAEFVHFACTSEDINNTSHALQLRSGRDQVILPGLDRIVLKLREMAHAYA
EVPMLSRTHGQTASPTTVGKELANVVVRLQAACDRIAGVKMLGKMNGAVGNYNAHLSAWPDFDWEAFSRKVVETPEPLGL
GLTFQPYSIQIEPHDYMAELFDAIARANTILVDLSRDIWGYVSLGYFKQRLKAGEIGSSTMPHKVNPIDFENAEGNLGLA
NALLRHLSEKLPVSRWQRDLTDSTVLRNIGVAMGYAALAYTSLMTGLNKLELNEEALAADLDASWEVLAEPIQTVMRRYG
VQGAYEKLKEVTRGKTVTAEALHALIAGLEIPQADKDRLLAMTPGSYVGKAAELARRV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=453, Percent_Identity=57.8366445916115, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50783; Mature: 50652

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSTINALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWFIALSDAGFEQFKPLTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCHHHCCCCCH
GARAYLLSLVKNFSEADSQAIKDIEKTTNHDVKAVEYWIKSKFEARPELERAAEFVHFAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
TSEDINNTSHALQLRSGRDQVILPGLDRIVLKLREMAHAYAEVPMLSRTHGQTASPTTVG
CCCCCCCCHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHH
KELANVVVRLQAACDRIAGVKMLGKMNGAVGNYNAHLSAWPDFDWEAFSRKVVETPEPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
LGLTFQPYSIQIEPHDYMAELFDAIARANTILVDLSRDIWGYVSLGYFKQRLKAGEIGSS
CCEEECCCEEEECCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
TMPHKVNPIDFENAEGNLGLANALLRHLSEKLPVSRWQRDLTDSTVLRNIGVAMGYAALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTSLMTGLNKLELNEEALAADLDASWEVLAEPIQTVMRRYGVQGAYEKLKEVTRGKTVTA
HHHHHHCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
EALHALIAGLEIPQADKDRLLAMTPGSYVGKAAELARRV
HHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSTINALSPLDGRYAAKLSALRPIMSEHGYMHRRVQVEVAWFIALSDAGFEQFKPLTT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCHHHCCCCCH
GARAYLLSLVKNFSEADSQAIKDIEKTTNHDVKAVEYWIKSKFEARPELERAAEFVHFAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
TSEDINNTSHALQLRSGRDQVILPGLDRIVLKLREMAHAYAEVPMLSRTHGQTASPTTVG
CCCCCCCCHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHH
KELANVVVRLQAACDRIAGVKMLGKMNGAVGNYNAHLSAWPDFDWEAFSRKVVETPEPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
LGLTFQPYSIQIEPHDYMAELFDAIARANTILVDLSRDIWGYVSLGYFKQRLKAGEIGSS
CCEEECCCEEEECCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
TMPHKVNPIDFENAEGNLGLANALLRHLSEKLPVSRWQRDLTDSTVLRNIGVAMGYAALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTSLMTGLNKLELNEEALAADLDASWEVLAEPIQTVMRRYGVQGAYEKLKEVTRGKTVTA
HHHHHHCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
EALHALIAGLEIPQADKDRLLAMTPGSYVGKAAELARRV
HHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]